Fabrication of reduced graphene oxide and sliver nanoparticle hybrids for Raman detection of absorbed folic acid: a potential cancer diagnostic probe.

Reduced graphene oxide (RGO) and silver nanoparticle (AgNP) hybrids (RGO-AgNP) were prepared by a facile one-pot method using Poly (N-vinyl-2-pyrrolidone) as reductant and stabilizer. Folic acid (FA) molecules were attached to the RGO-AgNP by physisorption for targeting specific cancer cells with folate receptors (FRs) and using as Raman reporter molecules. The internalization of the FA loaded RGO-AgNP (RGO-AgNP-FA) inside the FRs-positive cancer cell was confirmed by confocal laser scanning and transmission electron microscopy. The Raman signals of the FA in live cancer cells were detected by confocal Raman spectroscope at 514 nm excitation, indicating that the RGO-AgNP-FA material has great potential as a Raman probe for cancer diagnosis in vitro.

[1]  K. Kneipp,et al.  Surface-enhanced Raman scattering in local optical fields of silver and gold nanoaggregates-from single-molecule Raman spectroscopy to ultrasensitive probing in live cells. , 2006, Accounts of chemical research.

[2]  Xin Huang,et al.  Multi-functionalized graphene oxide based anticancer drug-carrier with dual-targeting function and pH-sensitivity , 2011 .

[3]  Chi-Ming Che,et al.  Proteomic analysis of the mode of antibacterial action of silver nanoparticles. , 2006, Journal of proteome research.

[4]  Can Xue,et al.  In Situ Synthesis of Metal Nanoparticles on Single-Layer Graphene Oxide and Reduced Graphene Oxide Surfaces , 2009 .

[5]  Lufeng Yang,et al.  Facile fabrication of carbonaceous nanospheres loaded with silver nanoparticles as antibacterial materials , 2012 .

[6]  L. Novotný,et al.  Subsurface Raman imaging with nanoscale resolution. , 2006, Nano letters.

[7]  Zhouyi Guo,et al.  Graphene oxide based surface-enhanced Raman scattering probes for cancer cell imaging. , 2013, Physical chemistry chemical physics : PCCP.

[8]  Jie Huang,et al.  Nanocomposites of size-controlled gold nanoparticles and graphene oxide: formation and applications in SERS and catalysis. , 2010, Nanoscale.

[9]  P. J. Ollivier,et al.  Layer-by-Layer Assembly of Ultrathin Composite Films from Micron-Sized Graphite Oxide Sheets and Polycations , 1999 .

[10]  Kai Yang,et al.  Graphene in mice: ultrahigh in vivo tumor uptake and efficient photothermal therapy. , 2010, Nano letters.

[11]  Xin Wang,et al.  Fabrication of flexible metal-nanoparticle films using graphene oxide sheets as substrates. , 2009, Small.

[12]  M. Otyepka,et al.  Interaction of Graphene and Arenes with Noble Metals , 2012 .

[13]  Paolo Navaretti,et al.  High Power and Stable High Coupling Efficiency (66%) Superluminescent Light Emitting Diodes by Using Active Multi-Mode Interferometer , 2011, IEICE Trans. Electron..

[14]  M. Otyepka,et al.  Functionalization of graphene: covalent and non-covalent approaches, derivatives and applications. , 2012, Chemical reviews.

[15]  Zhouyi Guo,et al.  Synergistic effect of chemo-photothermal therapy using PEGylated graphene oxide. , 2011, Biomaterials.

[16]  S. Nie,et al.  Single-molecule and single-nanoparticle SERS: from fundamental mechanisms to biomedical applications. , 2008, Chemical Society reviews.

[17]  R. J. Lee,et al.  Targeted drug delivery via the folate receptor. , 2000, Advanced drug delivery reviews.

[18]  Janina Kneipp,et al.  Surface-enhanced Raman scattering hybrid nanoprobe multiplexing and imaging in biological systems. , 2010, ACS nano.

[19]  A. Boisen,et al.  Non-covalent conjugates of single-walled carbon nanotubes and folic acid for interaction with cells over-expressing folate receptors. , 2013, Journal of materials chemistry. B.

[20]  A. G. S. Filho,et al.  Unveiling the Role of Oxidation Debris on the Surface Chemistry of Graphene through the Anchoring of Ag Nanoparticles , 2012 .

[21]  S. O’Brien,et al.  Excitation profile of surface-enhanced Raman scattering in graphene-metal nanoparticle based derivatives. , 2010, Nanoscale.

[22]  Olaf Hollricher,et al.  High-resolution, high-speed confocal Raman imaging , 2008 .

[23]  Anant Kumar Singh,et al.  Effect of Surface Coating on the Toxicity of Silver Nanomaterials on Human Skin Keratinocytes. , 2010, Chemical physics letters.

[24]  H. Dai,et al.  Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy. , 2011, Journal of the American Chemical Society.

[25]  Zhijun Zhang,et al.  Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs. , 2010, Small.

[26]  Rong Zhou,et al.  Iron oxide nanoparticles as magnetic resonance contrast agent for tumor imaging via folate receptor-targeted delivery. , 2004, Academic radiology.

[27]  Daxiang Cui,et al.  Folic acid-conjugated silica-modified gold nanorods for X-ray/CT imaging-guided dual-mode radiation and photo-thermal therapy. , 2011, Biomaterials.

[28]  Jürgen Popp,et al.  SERS: a versatile tool in chemical and biochemical diagnostics , 2008, Analytical and bioanalytical chemistry.

[29]  A. Srivastava,et al.  A facile and novel synthesis of Ag-graphene-based nanocomposites. , 2009, Small.

[30]  Zhe Zhang,et al.  A facile one-pot method to high-quality Ag-graphene composite nanosheets for efficient surface-enhanced Raman scattering. , 2011, Chemical communications.

[31]  Rizlan Bernier-Latmani,et al.  Binding of silver nanoparticles to bacterial proteins depends on surface modifications and inhibits enzymatic activity. , 2010, Environmental science & technology.

[32]  Zhuang Liu,et al.  PEGylated nanographene oxide for delivery of water-insoluble cancer drugs. , 2008, Journal of the American Chemical Society.

[33]  Yuehe Lin,et al.  Graphene and graphene oxide: biofunctionalization and applications in biotechnology , 2011, Trends in Biotechnology.

[34]  Yin Zhang,et al.  Molecular imaging with SERS-active nanoparticles. , 2011, Small.

[35]  Hua Zhang,et al.  Surface enhanced Raman scattering of Ag or Au nanoparticle-decorated reduced graphene oxide for detection of aromatic molecules , 2011 .

[36]  Ioan Notingher,et al.  Raman Spectroscopy Cell-based Biosensors , 2007, Italian National Conference on Sensors.

[37]  Zhuyuan Wang,et al.  Dual-mode probe based on mesoporous silica coated gold nanorods for targeting cancer cells. , 2011, Biosensors & bioelectronics.

[38]  W. Hu,et al.  Sulfonated Graphene for Persistent Aromatic Pollutant Management , 2011, Advanced materials.

[39]  S. Mhaisalkar,et al.  Hybrid graphene–metal nanoparticle systems: electronic properties and gas interaction , 2011 .

[40]  Sun-ho Han,et al.  Inorganic Drug‐Delivery Nanovehicle Conjugated with Cancer‐Cell‐Specific Ligand , 2009 .

[41]  Silke Krol,et al.  Synthesis and multidisciplinary characterization of polyelectrolyte multilayer-coated nanogold with improved stability toward aggregation , 2011 .

[42]  J. Rong,et al.  Fabrication of a graphene oxide–gold nanorod hybrid material by electrostatic self-assembly for surface-enhanced Raman scattering , 2013 .

[43]  G. Eda,et al.  Graphene oxide as a chemically tunable platform for optical applications. , 2010, Nature chemistry.

[44]  Xinxin Yu,et al.  Tuning chemical enhancement of SERS by controlling the chemical reduction of graphene oxide nanosheets. , 2011, ACS nano.

[45]  Qiyuan He,et al.  Graphene-based materials: synthesis, characterization, properties, and applications. , 2011, Small.

[46]  Clive G. Wilson,et al.  Surface-Enhanced Raman Scattering Spectroscopy as a Sensitive and Selective Technique for the Detection of Folic Acid in Water and Human Serum , 2008, Applied spectroscopy.

[47]  Xianzhi Fu,et al.  TiO2-graphene nanocomposites for gas-phase photocatalytic degradation of volatile aromatic pollutant: is TiO2-graphene truly different from other TiO2-carbon composite materials? , 2010, ACS nano.

[48]  Paolo Navaretti,et al.  High-Power ($> 110$ mW) Superluminescent Diodes by Using Active Multimode Interferometer , 2010, IEEE Photonics Technology Letters.

[49]  Wei Song,et al.  Functionalizing metal nanostructured film with graphene oxide for ultrasensitive detection of aromatic molecules by surface-enhanced Raman spectroscopy. , 2011, ACS applied materials & interfaces.

[50]  W. Guo,et al.  Efficient gene delivery via non-covalent complexes of folic acid and polyethylenimine. , 2001, Journal of controlled release : official journal of the Controlled Release Society.

[51]  Paolo Navaretti,et al.  Thermal resistance reduction in high power superluminescent diodes by using active multi-mode interferometer , 2012 .

[52]  Yin Zhang,et al.  Imaging with Raman spectroscopy. , 2010, Current pharmaceutical biotechnology.

[53]  Xiaoke Zhang,et al.  Targeted delivery and controlled release of doxorubicin to cancer cells using modified single wall carbon nanotubes. , 2009, Biomaterials.

[54]  Xuejiao Zhou,et al.  Individual nanocomposite sheets of chemically reduced graphene oxide and poly(N-vinyl pyrrolidone): preparation and humidity sensing characteristics , 2010 .

[55]  Hao Yan,et al.  Stable silver nanoparticle-DNA conjugates for directed self-assembly of core-satellite silver-gold nanoclusters. , 2009, Chemical communications.

[56]  I-Wei Chen,et al.  Quantum‐Dot‐Tagged Reduced Graphene Oxide Nanocomposites for Bright Fluorescence Bioimaging and Photothermal Therapy Monitored In Situ , 2012, Advanced materials.

[57]  Sanjiv S. Gambhir,et al.  Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopy , 2009, Proceedings of the National Academy of Sciences.

[58]  Lin Li,et al.  Water‐Soluble Poly(N‐isopropylacrylamide)–Graphene Sheets Synthesized via Click Chemistry for Drug Delivery , 2011 .

[59]  L. Kelemen,et al.  The role of folate receptor α in cancer development, progression and treatment: Cause, consequence or innocent bystander? , 2006, International journal of cancer.

[60]  Vikas Berry,et al.  Implantation and growth of dendritic gold nanostructures on graphene derivatives: electrical property tailoring and Raman enhancement. , 2009, ACS nano.

[61]  Wen Ren,et al.  A binary functional substrate for enrichment and ultrasensitive SERS spectroscopic detection of folic acid using graphene oxide/Ag nanoparticle hybrids. , 2011, ACS nano.