Cloning of 3′ end cDNA of ascorbate peroxidase gene from Fragaria × ananassa cv. Toyonaka.

A fragment of 3’ end cDNA of apx in Fragaria × ananassa cv. Toyonaka was cloned by rapid amplification of cDNA 3’ end (3’RACE) PCR. By joining the sequence with the known fragment sequence which we had submitted to GenBank (FJ896040), a 3’-end partial cDNA of 672bp was obtained, encoding 140 amino acids. The sequence contained the corresponding coding sequence of 424bp long including a stop codon TAA, and the 3’-untranslated region(3’UTR) of 248bp including a potential polyadenylation signal (AAATAA) and poly(A) of 28bp long. Homology analysis of nucleotide sequence and deduced amino acid sequence on the apx gene showed that it shared high homology with different plants species, among which it shared the highest sequence homology with Fragaria × ananassa cv. Yoho.