Analysis of DNA polymerase reaction products for detecting hepatitis B virus in serum—comparison with spot hybridization technique

An assay for DNA polymerase reaction products using slab gel electrophoresis and autoradiography was compared with the spot hybridization technique for the detection of hepatitis B virus DNA in 317 blood samples. The former could identify the nature and size of DNA on electrophoresis, and reduce potentially false‐positive results due to artifacts.

[1]  D. Shafritz,et al.  Analysis of Liver Disease, Nuclear HBcAg, Viral Replication, and Hepatitis B Virus DNA in Liver and Serum of HBcAg Vs. Anti‐HBe Positive Carriers of Hepatitis B Virus , 2007, Hepatology.

[2]  C. Bréchot,et al.  Detection of Hepatitis B Virus DNA in Serum by a Simple Spot Hybridization Technique: Comparison with Results for Other Viral Markers , 2007, Hepatology.

[3]  D. Shafritz,et al.  Detection of Hepatitis B Virus DNA Directly in Human Serum by a Simplified Molecular Hybridization Test: Comparison to HBeAg/ Anti‐HBe Status in HBsAg Carriers , 2007, Hepatology.

[4]  T. Merigan,et al.  Antiviral Treatment of Chronic Hepatitis B Virus Infection: Infectious Virus Cannot Be Detected in Patient Serum after Permanent Responses to Treatment , 2007, Hepatology.

[5]  I. Mushahwar,et al.  Radioimmunoassay for detection of hepatitis B e antigen and its antibody. Results of clinical evaluation. , 1981, American journal of clinical pathology.

[6]  J. Howie.  Significance of Escherichia coli in cheese. , 1981, Lancet.

[7]  C. Bréchot,et al.  DETECTION OF HEPATITIS B VIRUS DNA IN LIVER AND SERUM: A DIRECT APPRAISAL OF THE CHRONIC CARRIER STATE , 1981, The Lancet.

[8]  J. Gerin,et al.  Hepatitis B virus DNA in the sera of HBsAg carriers: A marker of active hepatitis B virus replication in the liver , 1981, Hepatology.

[9]  T. Merigan,et al.  Antiviral treatment of chronic hepatitis B virus infection. I. Changes in viral markers with interferon combined with adenine arabinoside. , 1981, The Journal of infectious diseases.

[10]  J. Summers,et al.  Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus , 1980, Journal of virology.

[11]  S. Pestka,et al.  Human leukocyte interferon produced by E. coli is biologically active , 1980, Nature.

[12]  G R Stark,et al.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[13]  J. Senior,et al.  Hepatitis B immune globulin for accidental exposures among medical personnel: final report of a multicenter controlled trial. , 1978, The Journal of infectious diseases.

[14]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[15]  M. Imai,et al.  e antigen and anti-e in the serum of asymptomatic carrier mothers as indicators of positive and negative transmission of hepatitis B virus to their infants. , 1976, The New England journal of medicine.

[16]  H. Greenlee,et al.  EFFICACY OF HEPATITIS B IMMUNE SERUM GLOBULIN AFTER ACCIDENTAL EXPOSURE Preliminary Report of the Veterans Administration Cooperative Study , 1975, The Lancet.

[17]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[18]  J. Summers,et al.  Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Y. Miyakawa,et al.  Hepatitis B surface antigen in the serum of infants after deliver from asymptomatic carrier mothers. , 1975, The Journal of pediatrics.

[20]  W. Robinson.  DNA and DNA polymerase in the core of the Dane particle of hepatitis B. , 1975, The American journal of the medical sciences.

[21]  R. Greenman,et al.  DNA Polymerase Associated with Human Hepatitis B Antigen , 1973, Journal of virology.

[22]  H. Thomas,et al.  Hepatitis B virus infection: the impact of molecular biology. , 1983, Hepatology.

[23]  H. Thomas,et al.  The detection of hbv‐dna in serum by molecular hybridisation: A more sensitive method for the detection of complete hbv particles , 1982, Journal of medical virology.

[24]  J. Gerin,et al.  An assay for the detection of the dna genome of hepatitis b virus in serum , 1982, Journal of Medical Virology.