Magnetic resonance imaging accurately estimates LV mass in a transgenic mouse model of cardiac hypertrophy.

Transgenic mice with a dysfunctional guanylyl cyclase A gene (GCA -/-) are unable to transduce the signals from atrial naturetic peptide and develop hypertension and cardiac hypertrophy. Magnetic resonance imaging (MRI) was performed to assess cardiac hypertrophy in these animals, using wild-type siblings as controls. Anesthetized mice were studied by gated multislice, multiphase cine MRI at 1.5 T. Simpson's rule was used to estimate left ventricle (LV) mass and volumes from short-axis images. Correlation between LV mass evaluated by MRI and at necropsy was excellent, with LVnecropsy = 1.04 × LVMRI + 4.69 mg ( r 2 = 0.95). By MRI, GCA -/- LV mass was significantly different when compared with isogenic controls [GCA -/-, 226 ± 43 mg ( n = 14) vs. controls, 156 ± 14 mg ( n = 10); P < 0.0001]. LV volumes and ejection fraction in the two groups were not significantly different. MRI provides an accurate means for the noninvasive assessment of murine cardiac phenotype and may be useful in following the effects of genetic modification.

[1]  H. Zerwes,et al.  Time Course of Spirapril‐Induced Structural and Functional Changes After Myocardial Infarction in Rats Followed with Magnetic Resonance Imaging , 1993, Journal of cardiovascular pharmacology.

[2]  R. Walsh,et al.  In vivo echocardiographic detection of enhanced left ventricular function in gene-targeted mice with phospholamban deficiency. , 1995, Circulation research.

[3]  S. Rose,et al.  High resolution high field rodent cardiac imaging with flow enhancement suppression. , 1994, Magnetic resonance imaging.

[4]  J Z Wang,et al.  MRI evaluation of left ventricular hypertrophy in a canine model of aortic stenosis. , 1990, Investigative radiology.

[5]  C. Lien,et al.  The enhanced Stark effects of coupled quantum wells and their application to tunable IR photodetectors , 1993 .

[6]  N. Reichek.  Echocardiographic assessment of left ventricular hypertrophy. , 1982, European heart journal.

[7]  J. Hestenes,et al.  Cross‐sectional Echocardiography 1. Analysis of Mathematic Models for Quantifying Mass of the Left Ventricle in Dogs , 1979, Circulation.

[8]  D. Altman,et al.  STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT , 1986, The Lancet.

[9]  J. Murgo,et al.  Microsphere and dilution techniques for the determination of blood flows and volumes in conscious mice. , 1992, The American journal of physiology.

[10]  N. Reichek,et al.  Echocardiographic assessment of left ventricular hypertrophy: comparison to necropsy findings. , 1986, The American journal of cardiology.

[11]  W. Hundley,et al.  Quantitative MR imaging of the heart. , 1996, Magnetic resonance imaging clinics of North America.

[12]  W J Manning,et al.  In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation. , 1994, The American journal of physiology.

[13]  D. Garbers,et al.  Salt-resistant hypertension in mice lacking the guanylyl cyclase-A receptor for atrial natriuretic peptide , 1995, Nature.

[14]  G. de Simone,et al.  Echocardiographic measurement of left ventricular mass and volume in normotensive and hypertensive rats. Necropsy validation. , 1990, American journal of hypertension.

[15]  R W Parkey,et al.  In vivo measurement of myocardial mass using nuclear magnetic resonance imaging. , 1986, Journal of the American College of Cardiology.

[16]  H. Gavras,et al.  Models of experimental hypertension in mice. , 1996, Hypertension.

[17]  T. Kurtz.  Genetic models of hypertension , 1994, The Lancet.

[18]  J. Gardin,et al.  Echocardiographic assessment of left ventricular mass and systolic function in mice. , 1995, Circulation research.

[19]  W. Davidson,et al.  Echocardiographic evaluation of size, function, and mass of normal and hypertrophied rat ventricles. , 1993, Journal of applied physiology.

[20]  J. Ross,et al.  Transthoracic echocardiography in models of cardiac disease in the mouse. , 1996, Circulation.

[21]  P. Douglas,et al.  Serial echocardiographic assessment of left ventricular geometry and function after large myocardial infarction in the rat. , 1994, Circulation.

[22]  R W Parkey,et al.  Estimation of human myocardial mass with MR imaging. , 1988, Radiology.

[23]  W. V. van Gilst,et al.  The effects of short- and long-term treatment with an ACE-inhibitor in rats with myocardial infarction. , 1991, Basic research in cardiology.

[24]  J. Gardin,et al.  Gated magnetic resonance imaging of normal and hypertrophied murine hearts. , 1997, The American journal of physiology.

[25]  A. Weyman.  Cross-sectional echocardiography , 1982 .