Investigating Class I Integron and Antimicrobial Resistance Profile of Klebsiella pneumonia isolates in Babol, North of Iran

the class I integrons. We also investigated the suitability of the two pairs of primers for the detection of the intl gene. Results : Antibiotic susceptibility testing revealed 90% resistance to ceftizoxime, cefotaxime, and cefepime, 88.6% to cefazolin, gentamicin, ticarcillin, and ceftriaxone, 83.3% to imipenem, 60% to ciprofloxacin, 56.6% to ofloxacin, and 36.6% to amikacin. The PCRs with two pairs of primers, one designed previously and the other in this study, detected int1 in 36.6% and 60% of samples, respectively. Conclusion: The int1 gene was of high prevalence (60%) in K. pneumoniae isolates. This factor could play a significant role in the spread of MDR strains. Also, failure to adhere to essential points in the design of the primer can lead to the production of primers with low specificity and efficiency, which reduces the proper identification of antibiotic resistance genes.

[1]  S. Khafri,et al.  Integron types, gene cassettes and antimicrobial resistance profile of Acinetobacter baumannii isolated from BAL samples in Babol, north of Iran. , 2017, Microbial pathogenesis.

[2]  A. Farivar,et al.  The study of antibiotic resistance among Klebsiella pneumoniae and expression level of oqxA and acrA genes by using real-time PCR , 2017 .

[3]  K. Nielsen,et al.  Global dissemination patterns of common gene cassette arrays in class 1 integrons. , 2015, Microbiology.

[4]  R. Rajabnia,et al.  Nosocomial emerging of (VIM1) carbapenemase-producing isolates of Klebsiella pneumoniae in North of Iran , 2015, Iranian journal of microbiology.

[5]  F. Brayner,et al.  Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco. , 2014, Revista da Sociedade Brasileira de Medicina Tropical.

[6]  Yongfei Hu,et al.  Structural Diversity of Class 1 Integrons and Their Associated Gene Cassettes in Klebsiella pneumoniae Isolates from a Hospital in China , 2013, PloS one.

[7]  M. Shafii,et al.  Class 1 Integron in Pseudomonas aeruginosa Isolates From Different Places and Devices of ICU in Babol, Iran , 2013 .

[8]  S. Pournaras,et al.  Global evolution of multidrug-resistant Acinetobacter baumannii clonal lineages. , 2013, International journal of antimicrobial agents.

[9]  A. Al-Thahab.  Molecular Detection of Extended-Spectrum Beta- Lactamases inClinical Isolates of Acinetobacterbaumannii , 2013 .

[10]  A. Mojtahedi,et al.  Molecular Detection of Integron Genes and Pattern of Antibiotic Resistance in Pseudomonas Aeruginosa Strains Isolated from Intensive Care Unit, Shahid Beheshti Hospital, North of Iran , 2012, International journal of molecular and cellular medicine.

[11]  M. Ahangarzadeh Rezaee,et al.  First report of class 1 and class 2 integrons in multidrug-resistant Klebsiella pneumoniae isolates from northwest Iran. , 2012, Japanese journal of infectious diseases.

[12]  S. Gharavi,et al.  Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010) , 2011 .

[13]  D. Lye,et al.  World health day 2011: antimicrobial resistance and practical solutions. , 2011, Annals of the Academy of Medicine, Singapore.

[14]  A. Japoni,et al.  Association between existence of integrons and multi-drug resistance in Acinetobacter isolated from patients in southern Iran. , 2011, Polish journal of microbiology.

[15]  J. Ayala,et al.  Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients. , 2010, International journal of antimicrobial agents.

[16]  V. Miriagou,et al.  Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system. , 2010, Journal of microbiological methods.

[17]  F. Fatemeh,et al.  Distribution of integrons among multidrug resistant E. coli and Klebsiella strains isolated from children with urinary tract infection. , 2010 .

[18]  J. D. Di Conza,et al.  [Integrons: gene collectors]. , 2010, Revista Argentina de microbiologia.

[19]  Timothy R. Walsh,et al.  Characterization of a New Metallo-β-Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India , 2009, Antimicrobial Agents and Chemotherapy.

[20]  S. Padmini,et al.  Evaluation of CIVA agar for rapid detection of extended spectrum beta- lactamases (ESBL) among isolates of Enterobacteriaceae. , 2008, The Indian journal of medical research.

[21]  J. Mcgowan,et al.  Class 1 Integrons in Resistant Escherichia coli and Klebsiella spp., US Hospitals , 2006, Emerging infectious diseases.

[22]  Clinical,et al.  Performance standards for antimicrobial disk susceptibility tests : approved standard , 2006 .

[23]  Didier Mazel,et al.  Integron cassette insertion: a recombination process involving a folded single strand substrate , 2005, The EMBO journal.

[24]  G. Emtiazi,et al.  Antimicrobial, heavy metal resistance and plasmid profile of coliforms isolated from nosocomial infections in a hospital in Isfahan, Iran , 2003 .

[25]  F. Baquero,et al.  Multi-resistant Gram-negative bacilli: from epidemics to endemics , 2003, Current opinion in infectious diseases.

[26]  J. Verhoef,et al.  Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin. , 2003, The Journal of infectious diseases.

[27]  R N Jones,et al.  Survey of infections due to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY Antimicrobial Surveillance Program, 1997-1999. , 2001, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[28]  H. Endtz,et al.  Rapid emergence of ciprofloxacin-resistant enterobacteriaceae containing multiple gentamicin resistance-associated integrons in a Dutch hospital. , 2001, Emerging infectious diseases.