Taxonomic study of a novel terrestrial alga, Spongiosarcinopsisqinghaiensis sp. nov. (Protosiphonaceae, Chlorophyta), from the Qinghai-Tibet Plateau

Abstract There is only one species of Spongiosarcinopsis in the literature currently. It was found in gray soil in Russia for the first time. According to molecular data analysis results, the isolated algal strain is most closely related to Spongiosarcinopsisterrestris. Unlike Spongiosarcinopsisterrestris, the isolated strain was found on soil surfaces at high altitudes, the young vegetative cell is spherical, vegetative cells are relatively large, and pyrenoids are generally fewer. In view of such morphological differences, phylogenetic analysis results, and comparison of ITS2 secondary structure and ultrastructure, the strain isolated in the present study was proposed to be a novel species.

[1]  Sudhir Kumar,et al.  MEGA11: Molecular Evolutionary Genetics Analysis Version 11 , 2021, Molecular biology and evolution.

[2]  Alexey M. Kozlov,et al.  ModelTest-NG: A New and Scalable Tool for the Selection of DNA and Protein Evolutionary Models , 2019, bioRxiv.

[3]  J. Jansson,et al.  Modern Soil Microbiology , 2019 .

[4]  Jin Zhang,et al.  PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies , 2018, bioRxiv.

[5]  A. Temraleeva,et al.  Spongiosarcinopsis terrestris gen. et sp. nov. (Chlorophyta, Chlorophyceae): a new genus of green algae from gray forest soil, Russia , 2018, Phytotaxa.

[6]  A. Temraleeva,et al.  Morphology, ecology, and 18S rDNA phylogeny of the green microalgal order Protosiphonales (Chlorophyceae, Chlorophyta) , 2017, Microbiology.

[7]  M. Thines,et al.  siMBa—a simple graphical user interface for the Bayesian phylogenetic inference program MrBayes , 2014, Mycological Progress.

[8]  F. Leliaert,et al.  DNA-based species delimitation in algae , 2014 .

[9]  K. Katoh,et al.  MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability , 2013, Molecular biology and evolution.

[10]  C. Bock,et al.  Phylogenetic position of Coronastrum ellipsoideum and description of Parachlorella hussii sp. nov. , 2011, Biologia.

[11]  C. Bock,et al.  UPDATING THE GENUS DICTYOSPHAERIUM AND DESCRIPTION OF MUCIDOSPHAERIUM GEN. NOV. (TREBOUXIOPHYCEAE) BASED ON MORPHOLOGICAL AND MOLECULAR DATA 1 , 2011, Journal of phycology.

[12]  Yann Ponty,et al.  VARNA: Interactive drawing and editing of the RNA secondary structure , 2009, Bioinform..

[13]  Thomas Dandekar,et al.  Synchronous visual analysis and editing of RNA sequence and secondary structure alignments using 4SALE , 2008, BMC Research Notes.

[14]  K. Misawa,et al.  Molecular systematics of Volvocales (Chlorophyceae, Chlorophyta) based on exhaustive 18S rRNA phylogenetic analyses. , 2008, Molecular phylogenetics and evolution.

[15]  Rodrigo Lopez,et al.  Clustal W and Clustal X version 2.0 , 2007, Bioinform..

[16]  T. Tengs,et al.  Phenotypically Different Microalgal Morphospecies with Identical Ribosomal DNA: A Case of Rapid Adaptive Evolution? , 2007, Microbial Ecology.

[17]  Koen Sabbe,et al.  THE SYSTEMATICS OF A SMALL SPINELESS DESMODESMUS SPECIES, D. COSTATO‐GRANULATUS (SPHAEROPLEALES, CHLOROPHYCEAE), BASED ON ITS2 rDNA SEQUENCE ANALYSES AND CELL WALL MORPHOLOGY 1 , 2007 .

[18]  M. Lürling.  Phenotypic plasticity in the green algae Desmodesmus and Scenedesmus with special reference to the induction of defensive morphology , 2003 .

[19]  S. Damberger,et al.  Nuclear-encoded rDNA group I introns: origin and phylogenetic relationships of insertion site lineages in the green algae. , 1996, Molecular biology and evolution.

[20]  M. Sogin,et al.  The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. , 1988, Gene.

[21]  B. Metting.  The systematics and ecology of soil algae , 1981, The Botanical Review.

[22]  F. R. Trainor,et al.  Ecology of soil algae: a review , 1981 .

[23]  M. M. Allen.  SIMPLE CONDITIONS FOR GROWTH OF UNICELLULAR BLUE‐GREEN ALGAE ON PLATES 1, 2 , 1968, Journal of phycology.

[24]  R. Brown,et al.  COMPARATIVE IMMUNOLOGY OF THE ALGAL GENERA TETRACYSTIS AND CHLOROCOCCUM 1 , 1965 .

[25]  W. R. Herndon.  STUDIES ON CHLOROSPHAERACEAN ALGAE FROM SOIL , 1958 .

[26]  David P. Parsons,et al.  Seaview Version 5: A Multiplatform Software for Multiple Sequence Alignment, Molecular Phylogenetic Analyses, and Tree Reconciliation. , 2021, Methods in molecular biology.

[27]  M. Nei,et al.  Molecular Evolutionary Genetics Analysis , 2007 .

[28]  G. G. Kuzyakhmetov.  Productivity of algocenoses in zonal arable soils of steppe and forest-steppe , 1998 .

[29]  R. C. Starr.  A comparative study of Chlorococcum meneghini, and other spherical, zoospore-producing genera of the Chlorococcales , 1955 .

[30]  A. Borzì.  Studi algologici : saggio di richerche sulla biologia delle alghe / Antonino Borzi. , 1883 .

[31]  F. Kützing.  Phycologia generalis : oder, Anatomie, physiologie und systemkunde der tange / Bearb. von Friedrich Traugott Kützing. Mit 80 farbig gedruckten tafeln, gezeichnet und gravirt vom verfasser. , 1843 .

[32]  C. A. Agardh.  Systema algarum. Adumbravit C. A. Adardh. , 1824 .