Multisample analysis using an array of microreactors for an alternating-current field-enhanced latex immunoassay.

To develop a rapid and multisample analysis system for latex immunoassay with submicroliters of sample, an array of microreactors were fabricated using micromachining techniques including photolithography, anisotropic etching, and thin gold film deposition. The chamber volume for immunoreactions of a single well was 0.4 microL. An alternating-current (ac) field was used to enhance the rate of the latex agglutination reaction. By applying an ac field for 1 min, alpha-fetoprotein in several samples could be determined simultaneously. The detection limit in this system was approximately 10 pg/mL.

[1]  I. Karube,et al.  Pulse immunoassay for human immunoglobulin G using antibody bound latex beads. , 1987, Biosensors.

[2]  I. Karube,et al.  Alternating current field enhanced latex immunoassay for human myoglobin as measured by image analysis , 1993 .

[3]  C. Cambiaso,et al.  [6] Particle counting immunoassay (PACIA) , 1981 .

[4]  R. Lauwerys,et al.  Comparison of turbidimetry with particle counting for the determination of human beta 2-microglobulin by latex immunoassay (LIA). , 1982, Clinica chimica acta; international journal of clinical chemistry.

[5]  Herman P. Schwan,et al.  Alternative‐Current Field‐Induced Forces and Their Biological Implications , 1969 .

[6]  Abelev Gi.  Alpha-Fetoprotein in Ontogenesis and its Association with Malignant Tumors , 1971 .

[7]  I. Karube,et al.  Pulse immunoassay for Candida albicans. , 1985, Analytical chemistry.

[8]  E. Grant,et al.  Dielectric relaxation of proteins in aqueous solutions , 1972 .

[9]  D. J. Harrison,et al.  Capillary electrophoresis and sample injection systems integrated on a planar glass chip , 1992 .

[10]  J. Grange,et al.  Nephelometric assay of antigens and antibodies with latex particles. , 1977, Journal of immunological methods.

[11]  C. Cambiaso,et al.  Particle counting immunoassay (PACIA). I. A general method for the determination of antibodies, antigens, and haptens. , 1977, Journal of immunological methods.

[12]  T. Waldmann,et al.  Effect of surgical and chemotherapeutic treatment on alpha‐fetoprotein levels in patients with hepatocellular carcinoma , 1976, Cancer.