Antioxidative properities of a traditional tincture and several leaf extracts of Allium ursinum L . ( collected in Montenegro and Bosnia and Herzegovina )

In order to estimate the amount of total phenols, flavonoids and the antioxidative properties of a traditional tincture, methanolic and ethanolic extracts from dried leaves of two different ecotypes of wild garlic (Allium ursinum L.), Folin-Ciocalteu method, AlCl3 method and DPPH radical scavenging assay were used. It was found that the type of extraction solvent affects the value of the detected bioactive compounds. The highest TPC, total flavonoid content and DPPH antiradical activity were found in 80% methanolic extract, 2111,11 mg GAE/100g DW, 20,00 mg QE/1g DW and EC50=77,21μg/ml, while the results obtained from the traditional tincture and 70% ethanolic extract were almost equal values. Significant correlation between the antioxidant activity and total flavonoid content (p>0.05; r=0.99) was found for a traditional tincture Received 09 Jul 2016, Revised 17 Nov 2016, Accepted 22 Nov 2016