论文信息 - Rapid Induction of Lymphoid Leukosis and Ascites by Lymphoid Leukosis Cell Line Avian Leukesis Virus from a

Rapid Induction of Lymphoid Leukosis and Ascites by Lymphoid Leukosis Cell Line Avian Leukesis Virus from a

ABsTRAcr, To examine whether a lymphoid lcukosis (LL) ceLL line releases an LL-specific avian teukosis virus (ALV) or not, two viral materia]s, cu]ture fiuid and a concentrated viral material from an LL-ceLl Line, were ineculated into a total of 74 day-old chicks of]ine l5I in 5 experiments. Spectrum of diseases induced, their incidence and incubation periods to onset were examined. Fifteen chicks weTe inoculated with the culture fluid and 9 (60%) developed ascites [59-1 ]9 days post lnoculation (dpi>; geometric mean (GM) of dpi, aM: 89,6)], but LL was not induced in any chicks inoculated, Fifty-nine chicks were inoculated with the concentrated viral material and LL was recognized in [3 (22.0%) (27-74 dpi; GM: 48,4), ascites with LL in ] 1 (18.6%) (3475 dpi; GM/4],3), ascites alone in 21 (35,6%) (32-83 dpl; GMi 482), erythroblastosis in 2 {3.4%) (70-102 dpl; GM/ 84.5), and other diseases in 12 (20.3%) (43-102 dpi; GM: 61,8). LL lesions were frcquently observed in the liver, spleen, kidneys, bursa of Fabricius (bursa), bone marrow and gonacis. Mild lyrnphocytic foci ifi svme visceral organs and perivascular cuffing in the central nerveus system were observed mainly in several chicks diagnesed as having complicalion of ascites with LL or otheT diseases. In addition to these lesion$, atrophy of bursa and thymuses was recognized in them. No antibodies against Marek's disease virus (MDV) and reticuloendotheliosis virus were detectc/d in 36 sera taken from the chicks inoculated with the concentrated virat material, Serotype 2 MDV was tsolated frorn the buffy coat of some inoculated chicks. 11hese

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