Engineering Designer Nucleases with Customized Cleavage Specificities

Engineered designer nucleases can be used to efficiently modify genomic sequence in a wide variety of model organisms and cell types. Zinc finger nucleases (ZFNs), consisting of an engineered zinc finger array fused to a non‐specific cleavage domain, have been extensively used to modify a broad range of endogenous genes. Protocols for engineering ZFNs targeted to specific gene sequences of interest using the context‐dependent assembly (CoDA) method are described in this unit. Curr. Protoc. Mol. Biol. 96:12.13.1‐12.13.16. © 2011 by John Wiley & Sons, Inc.

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