the Angiotensinogen Gene Associated Changes of DNA Methylation in - Dynamic CCAAT/Enhancer Binding Protein

—DNA methylation patterns are maintained in adult somatic cells. Recent findings, however, suggest that all methylation patterns are not preserved. We demonstrate that stimulatory signals can change the DNA methylation status at a CCAAT/enhancer binding protein (CEBP) binding site and a transcription start site and activate expression of the angiotensinogen gene ( AGT ). A CEBP binding site in the human AGT promoter was hypomethylated in tissues with high expression of AGT , but not in those with low expression. The transcriptional activity of AGT promoter sequences cloned into a reporter plasmid depended on DNA methylation. In cultured human cells, interleukin 6 stimulation caused DNA demethylation around a CEBP binding site and a transcription start site; demethylation was accompanied by increased CEBP- β recruitment and chromatin accessibility of the AGT promoter. DNA methylation activity decreased in the nucleus. Excess circulating aldosterone upregulated AGT expression and was accompanied by DNA hypomethylation around a CEBP binding site and a transcription start site in human visceral adipose tissue. High salt intake led to upregulation of Agt expression, DNA hypomethylation around 2 CEBP binding sites and a transcription start site, and decreased DNA methylation activity in rat visceral adipose tissue. Taken together, CEBP binding initiates chromatin relaxation and transcription, which are followed by DNA demethylation around a CEBP binding site and a transcription start site in the AGT promoter. Decreased DNA methylation activity in the nucleus may play a role in DNA demethylation. DNA demethylation switches the phenotype of AGT expression from an inactive to an active state. ( Hypertension . 2014;63:281-288.) performed using antibodies against USF1, ESR1, CEBPB (C/EBP β ), NR3C1 (GR), HNF4A, NR2F1 and MECP2 in both H295R and HepG2 cells. Percent change in transcription factors binding activities with methylated probes compared to those with unmethylated probes is shown.

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