Quantification of temporary and permanent subpopulations of bull sperm by an optimized SYBR‐14/propidium iodide assay

The quality of bull sperm is a key factor in the field of controlled reproduction. Viability‐testing is an important aspect of sperm quality definition, especially after cryopreservation where multiple factors such as handling, freeze‐thaw cycle, and preservation media, have an impact on the metabolic and functional state of sperm cells.

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