Development of an immunochromatographic strip assay for ractopamine detection using an ultrasensitive monoclonal antibody

ABSTRACT A sensitive gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed for the detection of ractopamine (RCT), a beta-adrenergic agonist. The immunogen and coating antigen were synthesized by the carbodiimide method and conjugated with keyhole limpet hemocyanin and ovalbumin, respectively. The highly sensitive and specific monoclonal antibody was prepared for RCT, with a 50% inhibition concentration of 0.05 ng/ml and had no cross-reactivity with other beta-adrenergic agonists. An ultrasensitive and rapid immunochromatographic strip assay was developed with an RCT cutoff value of 2 ng/ml. Both developed methods can be used for RCT detection in swine urine.

[1]  Suxia Zhang,et al.  New haptens and antibodies for ractopamine. , 2015, Food chemistry.

[2]  Juan Peng,et al.  Comparsion of an immunochromatographic strip with ELISA for simultaneous detection of thiamphenicol, florfenicol and chloramphenicol in food samples. , 2015, Biomedical chromatography : BMC.

[3]  Liqiang Liu,et al.  Development of an anti-chlorothalonil monoclonal antibody based on a novel designed hapten , 2015 .

[4]  Juan Peng,et al.  Antibody for the development of specific immunoassays to detect nadifloxacin in chicken muscles , 2015 .

[5]  A. Tret’yakov,et al.  QuEChERS sample preparation in the simultaneous determination of diethylstilbestrol and ractopamine in food by gas-liquid chromatography , 2015, Journal of Analytical Chemistry.

[6]  Liqiang Liu,et al.  Development of an ELISA for nitrazepam based on a monoclonal antibody , 2015 .

[7]  D. Arrigan,et al.  Electrochemical detection of ractopamine at arrays of micro-liquid | liquid interfaces. , 2015, Talanta.

[8]  Feng Xue,et al.  Development of a monoclonal antibody-based immunochromatographic strip for cephalexin , 2015 .

[9]  Jing Zhang,et al.  Preparation of ractopamine-tetraphenylborate complexed nanoparticles used as sensors to rapidly determine ractopamine residues in pork , 2014, Nanoscale Research Letters.

[10]  Mao‐xiang Jing,et al.  High performance of carbon nanotubes/silver nanowires-PET hybrid flexible transparent conductive films via facile pressing-transfer technique , 2014, Nanoscale Research Letters.

[11]  Lie-Chwen Lin,et al.  Pharmacokinetics of ractopamine and its organ distribution in rats. , 2014, Journal of agricultural and food chemistry.

[12]  Shijia Yang,et al.  Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol. , 2014, Analytica chimica acta.

[13]  Xiaoping Yu,et al.  Development of a monoclonal antibody-based direct competitive enzyme-linked immunosorbent assay for a new β-adrenergic agonist phenylethanolamine A , 2014 .

[14]  Changlong Hao,et al.  A highly sensitive enzyme-linked immunosorbent assay for copper(II) determination in drinking water , 2014 .

[15]  Qiang Fu,et al.  Combined microextraction by packed sorbent and high-performance liquid chromatography-ultraviolet detection for rapid analysis of ractopamine in porcine muscle and urine samples. , 2014, Food chemistry.

[16]  Hengyi Xu,et al.  Lateral flow immunoassay for quantitative detection of ractopamine in swine urine. , 2014, Biomedical and environmental sciences : BES.

[17]  Xiao-yun Liu,et al.  Identification of ractopamine glucuronides and determination of bioactive ractopamine residues and its metabolites in food animal urine by ELISA, LC-MS/MS and GC-MS , 2014, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[18]  Bin Du,et al.  A silver-palladium alloy nanoparticle-based electrochemical biosensor for simultaneous detection of ractopamine, clenbuterol and salbutamol. , 2013, Biosensors & bioelectronics.

[19]  Anping Deng,et al.  Development of a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection of phenylethanolamine A in tissue and feed samples and confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). , 2013, Talanta.

[20]  Shen-Ming Chen,et al.  Electrochemical detection of toxic ractopamine and salbutamol in pig meat and human urine samples by using poly taurine/zirconia nanoparticles modified electrodes. , 2013, Colloids and surfaces. B, Biointerfaces.

[21]  Liqiang Liu,et al.  Development and validation of a sandwich ELISA for quantification of peanut agglutinin (PNA) in foods , 2012 .

[22]  Hongtao Lei,et al.  Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork. , 2012, Analytica chimica acta.

[23]  Liqiang Liu,et al.  Rapid on‐site determination of melamine in raw milk by an immunochromatographic strip , 2012 .

[24]  Lei Liu,et al.  Development of indirect competitive immunoassay for highly sensitive determination of ractopamine in pork liver samples based on surface plasmon resonance sensor , 2012 .

[25]  Chunhai Fan,et al.  A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol. , 2011, Biosensors & bioelectronics.

[26]  Jiadong Huang,et al.  Electrochemical immunosensor for salbutamol detection based on CS-Fe3O4-PAMAM-GNPs nanocomposites and HRP-MWCNTs-Ab bioconjugates for signal amplification , 2011 .

[27]  X. Xia,et al.  Validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for determination of ractopamine: Application to residue depletion study in swine , 2011 .

[28]  Guozhen Fang,et al.  Substitution of Antibody with Molecularly Imprinted Film in Enzyme-Linked Immunosorbent Assay for Determination of Trace Ractopamine in Urine and Pork Samples , 2011 .

[29]  Ruijin Liu,et al.  Investigation of ractopamine-imprinted polymer for dispersive solid-phase extraction of trace beta-agonists in pig tissues. , 2010, Journal of separation science.

[30]  D. Zhao,et al.  Development of rapid immunoassays for the detection of ractopamine in swine urine , 2010, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[31]  Linhai Wu,et al.  Multi-residue detection of benzodiazepines by ELISA based on class selective antibodies , 2009 .

[32]  M. Himmelsbach,et al.  A sensitive non-aqueous capillary electrophoresis-mass spectrometric method for multiresidue analyses of beta-agonists in pork. , 2009, Biomedical chromatography : BMC.

[33]  Chifang Peng,et al.  Production of new class-specific polyclonal antibody for determination of fluoroquinolones antibiotics by indirect competitive ELISA , 2008 .

[34]  Chifang Peng,et al.  Fragment-based hapten design and screening of a highly sensitive and specific monoclonal antibody for ractopamine , 2014 .

[35]  S. Hostiuc,et al.  Study regarding drugs in blood with ELISA and chemiluminiscence versus ELISA with spectrophotometric detection , 2012 .