Cineraria maritima Linn. is an important medicinal plant of known therapeutic value for the treatment of cataract and corneal opacity. An in vitro regeneration protocol has been standardized for large-scale supply of planting material of this otherwise scarce medicinal plant species. The medium for propagation contained MS salts, B 5 vitamins, 30 g l - 1 sucrose and 8.0 g l - 1 agar (designated as MSB medium). Addition of thidiazuron [TDZ: N-phenyl-N'-(1,2,3-thidiazol-5-yl) urea] in the culture medium proved superior to the combined treatments of 6-benzyladenine and α-napthaleneacetic acid. The highest adventitious shoot bud (36 ′ 2.34) induction, per nodal explant used, occurred at 4.54 pM TDZ after 6 weeks of incubation. The number of shoots formed per explant increased significantly upon sub-culture of the responding explants on plant growth regulator-free MSB medium, after 8 weeks of culture initiation. In vitro produced shoots exhibited good rooting response on half strength MSB medium containing 4.92 μM indole 3-butyric acid. After 3 weeks of hardening of plantlets as hydrophonic cultures, almost 95% of 300 rooted plants could be successfully transferred and acclimatized ex vitro under glass-house conditions, followed by their establishment in the field.