Five years later: The current status of the use of proteomics and transcriptomics in EMF research

The World Health Organization's and Radiation and Nuclear Safety Authority's “Workshop on Application of Proteomics and Transcriptomics in Electromagnetic Fields Research” was held in Helsinki in the October/November 2005. As a consequence of this meeting, Proteomics journal published in 2006 a special issue “Application of Proteomics and Transcriptomics in EMF Research” (Vol. 6 No. 17; Guest Editor: D. Leszczynski). This Proteomics issue presented the status of research, of the effects of electromagnetic fields (EMF) using proteomics and transcriptomics methods, present in 2005. The current overview/opinion article presents the status of research in this area by reviewing all studies that were published by the end of 2010. The review work was a part of the European Cooperation in the Field of Scientific and Technical Research (COST) Action BM0704 that created a structure in which researchers in the field of EMF and health shared knowledge and information. The review was prepared by the members of the COST Action BM0704 task group on the high‐throughput screening techniques and electromagnetic fields (TG‐HTST‐EMF).

[1]  Thomas Burke,et al.  Human Biospecimen Research: Experimental Protocol and Quality Control Tools , 2009, Cancer Epidemiology Biomarkers & Prevention.

[2]  Blair Henderson,et al.  Gene expression profiling of human endothelial cells exposed to 50-Hz magnetic fields fails to produce regulated candidate genes , 2006, Cell stress & chaperones.

[3]  Norbert Pallua,et al.  Chiparray-basierte Identifikation von differenziell exprimierten Genen in humanen Nabelschnurendothelzellen (HUVEC) nach in vitro Stromexposition , 2006 .

[4]  Niels Kuster,et al.  Gene expression changes in human cells after exposure to mobile phone microwaves , 2006, Proteomics.

[5]  Reilly Jp.  Comments concerning "Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz)". , 1999 .

[6]  T Suhara,et al.  Phosphorylation and gene expression of p53 are not affected in human cells exposed to 2.1425 GHz band CW or W‐CDMA modulated radiation allocated to mobile radio base stations , 2006, Bioelectromagnetics.

[7]  S. Carr,et al.  A pipeline that integrates the discovery and verification of plasma protein biomarkers reveals candidate markers for cardiovascular disease , 2011, Nature Biotechnology.

[8]  Byoung-Tak Zhang,et al.  Characterization of biological effect of 1763 MHz radiofrequency exposure on auditory hair cells , 2008, International journal of radiation biology.

[9]  Dariusz Leszczynski,et al.  Mobile phone radiation causes changes in gene and protein expression in human endothelial cell lines and the response seems to be genome‐ and proteome‐dependent , 2006, Proteomics.

[10]  Sakari Joenväärä,et al.  Proteomics: new way to to determine possible biological effects of mobile phone radiation , 2001, Nature Genetics.

[11]  R. Aebersold,et al.  Applying mass spectrometry-based proteomics to genetics, genomics and network biology , 2009, Nature Reviews Genetics.

[12]  Christopher Gerner,et al.  Increased protein synthesis by cells exposed to a 1,800-MHz radio-frequency mobile phone electromagnetic field, detected by proteome profiling , 2010, International archives of occupational and environmental health.

[13]  Jon Brock,et al.  Facial Identity Recognition in the Broader Autism Phenotype , 2010, PloS one.

[14]  Byoung-Tak Zhang,et al.  Molecular responses of Jurkat T-cells to 1763 MHz radiofrequency radiation , 2008, International journal of radiation biology.

[15]  Deqiang Lu,et al.  Studying gene expression profile of rat neuron exposed to 1800MHz radiofrequency electromagnetic fields with cDNA microassay. , 2007, Toxicology.

[16]  Dariusz Leszczynski,et al.  Questions and answers concerning applicability of proteomics and transcriptomics in EMF research , 2006, Proteomics.

[17]  C. E. Parker,et al.  Mass-spectrometry-based clinical proteomics--a review and prospective. , 2010, The Analyst.

[18]  Niels Kuster,et al.  Analysis of proteome response to the mobile phone radiation in two types of human primary endothelial cells , 2010, Proteome Science.

[19]  M. Meltz,et al.  Nuclear Translocation and DNA-Binding Activity of NFKB (NF-κB) after Exposure of Human Monocytes to Pulsed Ultra-wideband Electromagnetic Fields (1 kV/cm) Fails to Transactivate κB-Dependent Gene Expression , 2006, Radiation research.

[20]  Donald K Martin,et al.  An in vitro study of the effects of exposure to a GSM signal in two human cell lines: Monocytic U937 and neuroblastoma SK‐N‐SH , 2006, Cell biology international.

[21]  Christoph H Borchers,et al.  Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma , 2009, Nature Biotechnology.

[22]  Dariusz Leszczynski,et al.  Mobile phone radiation might alter protein expression in human skin , 2008, BMC Genomics.

[23]  P. A. Mason,et al.  Gene Expression Changes in the Skin of Rats Induced by Prolonged 35 GHz Millimeter-Wave Exposure , 2008, Radiation research.

[24]  Weimin Gao,et al.  Effects of a strong static magnetic field on bacterium Shewanella oneidensis: An assessment by using whole genome microarray , 2005, Bioelectromagnetics.

[25]  Ke Yao,et al.  Proteomic Analysis of Human Lens Epithelial Cells Exposed to Microwaves , 2007, Japanese Journal of Ophthalmology.

[26]  J P McNamee,et al.  Evaluating the Biological Effects of Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Fields in a Series of Human-Derived Cell Lines , 2007, Radiation research.

[27]  Myrtill Simkó,et al.  Gene expression analysis of ELF-MF exposed human monocytes indicating the involvement of the alternative activation pathway. , 2006, Biochimica et biophysica acta.

[28]  P. Knapp,et al.  Exposure to cell phone radiation up-regulates apoptosis genes in primary cultures of neurons and astrocytes , 2007, Neuroscience Letters.

[29]  J P McNamee,et al.  Microarray Gene Expression Profiling of a Human Glioblastoma Cell Line Exposed In Vitro to a 1.9 GHz Pulse-Modulated Radiofrequency Field , 2006, Radiation research.

[30]  N. Anderson,et al.  The clinical plasma proteome: a survey of clinical assays for proteins in plasma and serum. , 2010, Clinical chemistry.

[31]  Duccio Cavalieri,et al.  Extremely Low-Frequency Electromagnetic Fields do not Affect DNA Damage and Gene Expression Profiles of Yeast and Human Lymphocytes , 2005, Radiation research.

[32]  Melanie Y. White,et al.  The Role of Proteomics in Clinical Cardiovascular Biomarker Discovery* , 2008, Molecular & Cellular Proteomics.

[33]  Xu Shi,et al.  Quantification of Cardiovascular Biomarkers in Patient Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution* , 2009, Molecular & Cellular Proteomics.

[34]  K. Coombs.  Quantitative proteomics of complex mixtures , 2011, Expert review of proteomics.

[35]  Niels Kuster,et al.  Proteomic Analysis of the Response of Human Endothelial Cell Line EA.hy926 to 1800 GSM Mobile Phone Radiation , 2009 .

[36]  Eiichiro Ichiishi,et al.  The effect of high strength static magnetic fields and ionizing radiation on gene expression and DNA damage in Caenorhabditis elegans , 2008, Bioelectromagnetics.

[37]  Hyung-Do Choi,et al.  Two-dimensional electrophoretic analysis of radio-frequency radiation-exposed MCF7 breast cancer cells. , 2010, Journal of radiation research.

[38]  David H. Sliney,et al.  Health issues related to the use of hand-held radiotelephones and base transmitters. International Commission on Non-Ionizing Radiation Protection. , 1996, Health physics.

[39]  Steven A Carr,et al.  Protein biomarker discovery and validation: the long and uncertain path to clinical utility , 2006, Nature Biotechnology.

[40]  Giorgi Bit-Babik,et al.  Low‐intensity microwave irradiation does not substantially alter gene expression in late larval and adult Caenorhabditis elegans , 2009, Bioelectromagnetics.

[41]  E. Moros,et al.  The number of genes changing expression after chronic exposure to Code Division Multiple Access or Frequency DMA radiofrequency radiation does not exceed the false‐positive rate , 2006, Proteomics.

[42]  M. Port,et al.  Influence of high‐frequency electromagnetic fields on different modes of cell death and gene expression , 2003, International journal of radiation biology.

[43]  Dongquan Chen,et al.  Transcriptional response of dermal fibroblasts in direct current electric fields , 2008, Bioelectromagnetics.

[44]  Lingli Wang,et al.  Effects of Global System for Mobile Communications 1800 MHz radiofrequency electromagnetic fields on gene and protein expression in MCF‐7 cells , 2006, Proteomics.

[45]  Craig Laramee,et al.  Effect of Power-Frequency Magnetic Fields on Genome-Scale Gene Expression in Saccharomyces cerevisiae , 2003, Radiation research.

[46]  Catrin Bauréus Koch,et al.  Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation , 2006, Bioelectromagnetics.

[47]  Dariusz Leszczynski,et al.  Proteomics analysis of human endothelial cell line EA.hy926 after exposure to GSM 900 radiation , 2004, Proteomics.

[48]  Andrew Williams,et al.  Analysis of gene expression in two human‐derived cell lines exposed in vitro to a 1.9 GHz pulse‐modulated radiofrequency field , 2007, Proteomics.

[49]  A. Ahlbom.  Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz) , 1998 .

[50]  Dariusz Leszczynski,et al.  Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: molecular mechanism for cancer- and blood-brain barrier-related effects. , 2002, Differentiation; research in biological diversity.

[51]  Ivano Bertini,et al.  Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks , 2011, Journal of biomolecular NMR.

[52]  S. Carr,et al.  Quantitative, Multiplexed Assays for Low Abundance Proteins in Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution*S , 2007, Molecular & Cellular Proteomics.

[53]  V. Romano Spica,et al.  No evidence of major transcriptional changes in the brain of mice exposed to 1800 MHz GSM signal , 2008, Bioelectromagnetics.

[54]  M. Gulisano,et al.  Exposure to global system for mobile communication (GSM) cellular phone radiofrequency alters gene expression, proliferation, and morphology of human skin fibroblasts. , 2002, Oncology research.

[55]  Alan R. Bishop,et al.  Mammalian Stem Cells Reprogramming in Response to Terahertz Radiation , 2010, PloS one.

[56]  Alvis Brazma,et al.  Minimum Information About a Microarray Experiment (MIAME) – Successes, Failures, Challenges , 2009, TheScientificWorldJournal.

[57]  Dieter Stoll,et al.  Proteome wide screening using peptide affinity capture , 2009, Proteomics.

[58]  Norbert Schütze,et al.  Effects of Weak, Low-Frequency Pulsed Electromagnetic Fields (BEMER Type) on Gene Expression of Human Mesenchymal Stem Cells and Chondrocytes: An In Vitro Study , 2007, Electromagnetic biology and medicine.

[59]  Dariusz Leszczynski,et al.  Applicability of discovery science approach to determine biological effects of mobile phone radiation , 2004, Proteomics.

[60]  T. Mandal,et al.  Radioprotection in mice following oral administration of WR-1065/PLGA nanoparticles , 2008, International journal of radiation biology.

[61]  E. Moros,et al.  Gene Expression does not Change Significantly in C3H 10T½ Cells after Exposure to 847.74 CDMA or 835.62 FDMA Radiofrequency Radiation , 2006, Radiation research.

[62]  Toshio Nojima,et al.  2-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells. , 2010, Journal of radiation research.

[63]  Rainer Hedrich,et al.  Is gene activity in plant cells affected by UMTS-irradiation? A whole genome approach , 2008, Advances and applications in bioinformatics and chemistry : AABC.

[64]  Pei Wang,et al.  The interface between biomarker discovery and clinical validation: The tar pit of the protein biomarker pipeline , 2008, Proteomics. Clinical applications.

[65]  Christina Schraml,et al.  Do static or time‐varying magnetic fields in magnetic resonance imaging (3.0 T) alter protein–gene expression?—A study on human embryonic lung fibroblasts , 2007, Journal of magnetic resonance imaging : JMRI.

[66]  Sung-Ho Myung,et al.  Lack of a co-promotion effect of 60 Hz circularly polarized magnetic fields on spontaneous development of lymphoma in AKR mice. , 2010, Bioelectromagnetics.