Monitoring of cell cultures with LTCC microelectrode array

AbstractMonitoring of cell cultures in microbioreactors is a crucial task in cell bioassays and toxicological tests. In this work a novel tool based on a miniaturized sensor array fabricated using low-temperature cofired ceramics (LTCC) technology is presented. The developed device is applied to the monitoring of cell-culture media change, detection of the growth of various species, and in toxicological studies performed with the use of cells. Noninvasive monitoring performed with the LTCC microelectrode array can be applied for future cell-engineering purposes. FigureMicroelectrode array for monitoring of cell cultures

[1]  L. Griffith,et al.  Capturing complex 3D tissue physiology in vitro , 2006, Nature Reviews Molecular Cell Biology.

[2]  Artur Dybko,et al.  LTCC based microfluidic system with optical detection , 2005 .

[3]  P. Ciosek,et al.  Miniaturized electronic tongue with an integrated reference microelectrode for the recognition of milk samples. , 2008, Talanta.

[4]  S. Giselbrecht,et al.  3D tissue culture substrates produced by microthermoforming of pre-processed polymer films , 2006, Biomedical microdevices.

[5]  Mengsu Yang,et al.  Microfluidics technology for manipulation and analysis of biological cells , 2006 .

[6]  B. Chung,et al.  Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. , 2005, Lab on a chip.

[7]  P. Ciosek,et al.  Polymeric membrane ion-selective and cross-sensitive electrode-based electronic tongue for qualitative analysis of beverages. , 2004, The Analyst.

[8]  Hanry Yu,et al.  A practical guide to microfluidic perfusion culture of adherent mammalian cells. , 2007, Lab on a chip.

[9]  L. Golonka,et al.  Microelectrode array fabricated in low temperature cofired ceramic (LTCC) technology , 2009 .

[10]  A. Dybko,et al.  Potentiometric electronic tongue based on integrated array of microelectrodes , 2007 .

[11]  Binil Starly,et al.  Enabling Sensor Technologies for the Quantitative Evaluation of Engineered Tissue , 2007, Annals of Biomedical Engineering.

[12]  Robert L Sah,et al.  Probing the role of multicellular organization in three-dimensional microenvironments , 2006, Nature Methods.

[13]  Kiyoshi Toko,et al.  Study of sweet taste evaluation using taste sensor with lipid/polymer membranes. , 2004, Biosensors & bioelectronics.

[14]  Zbigniew Brzózka,et al.  Analysis of dialysate fluids with the use of a potentiometric electronic tongue , 2008 .

[15]  Teruo Fujii,et al.  Microfluidic PDMS (Polydimethylsiloxane) Bioreactor for Large‐Scale Culture of Hepatocytes , 2004, Biotechnology progress.

[16]  Takehiko Kitamori,et al.  Biological cells on microchips: new technologies and applications. , 2007, Biosensors & bioelectronics.

[17]  H. Andersson,et al.  Microfluidic devices for cellomics: a review , 2003 .

[18]  Jun Wang,et al.  Recent advances in electric analysis of cells in microfluidic systems , 2008, Analytical and bioanalytical chemistry.

[19]  G. Shi,et al.  A water-soluble cationic oligopyrene derivative : Spectroscopic studies and sensing applications , 2009 .

[20]  W. Ehrfeld.  Electrochemistry and microsystems , 2003 .

[21]  E. Okine,et al.  Mature adipocytes may be a source of stem cells for tissue engineering. , 2008, Biochemical and biophysical research communications.

[22]  E. A. Cavalcanti-Adam,et al.  Cellular Chemomechanics at Interfaces: Sensing, Integration and Response{ , 2006 .

[23]  H. Lodish.  Molecular Cell Biology , 1986 .

[24]  P. Ciosek,et al.  The recognition of beer with flow-through sensor array based on miniaturized solid-state electrodes. , 2006, Talanta.

[25]  Patrycja Ciosek,et al.  The Recognition of Growth Conditions and Metabolic Type of Plants by a Potentiometric Electronic Tongue , 2006 .