Effects of Multi-Bacteria Solid-State Fermented Diets with Different Crude Fiber Levels on Growth Performance, Nutrient Digestibility, and Microbial Flora of Finishing Pigs

Simple Summary Dietary cellulase was found to be an important nutrient, and solid-state fermentation could improve the nutritional value of feed. To study the effects of multi-bacteria solid-state fermented diets and dietary crude fiber levels on finishing pigs, a total of 36 pigs were divided into four treatments: (1) pigs fed a basal diet containing 7.00% CF (HF), (2) pigs fed a basal multi-bacteria fermentation diet containing 7.00% CF (HFM), (3) pigs fed a basal diet containing 2.52% CF (LF), and (4) piglets fed a basal multi-bacteria fermentation diet containing 2.52% CF (LFM). The growth performance, nutrient digestibility and digestion amount, serum biochemical index, and fecal microflora were evaluated. Multi-bacteria solid-state fermentation had a positive effect on the nutrient digestion and serum biochemical indicators, which was contrary to high-fiber diets. Both high-fiber diets and multi-bacteria solid-state fermentation could optimize intestinal flora in finishing pigs. Abstract This study aimed to investigate the effects of multi-bacteria solid-state fermented diets with different crude fiber (CF) levels on growth performance, nutrient digestibility, and microbial flora of finishing pigs. The multi-bacteria solid-state fermented diets were made up of Lactobacillus amylovorus, Enterococcus faecalis, Bacillus subtilis, and Candida utilis. According to a 2 (factors) × 2 (levels) design, with the two factors being multi-bacteria solid-state fermentation (fed non-fermented diet or multi-bacteria fermentation) or CF levels (fed a basal diet containing 2.52% CF or 7.00% CF), a total of 36 finishing pigs (70.80 ± 5.75 kg) were divided into 4 treatments with 9 barrows per group: (1) pigs fed a diet containing 7.00% CF (HF), (2) pigs fed a multi-bacteria fermentation diet containing 7.00% CF (HFM), (3) pigs fed a diet containing 2.52% CF (LF), and (4) piglets fed a multi-bacteria fermentation diet containing 2.52% CF (LFM). This experiment lasted 28 days. The multi-bacteria solid-state fermented diet increased the backfat thickness (p < 0.05) and apparent total tract nutrient digestibility (ATTD) of CF, neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), 8 amino acids (Trp, Asp, Gly, Cys, Val, Met, Ile, and Leu), total essential amino acids (EAA), total non-essential amino acids (NEEA), and total amino acids (TAA) (p < 0.05). Multi-bacteria solid-state fermented diet increased serum concentrations of HDL-c, ABL, TP, and GLU, the serum enzyme activities of GSH-Px, T-AOC, SOD, and CAT (p < 0.05), the relative abundance of Lactobacillus, Oscillospira, and Coprococcus (p < 0.05), and the abundance of YAMINSYN3-PWY, PWY-7013, GOLPDLCAT-PWY, ARGORNPROST-PWY, and PWY-5022 pathways (p < 0.05). The multi-bacteria solid-state fermented diet reduced the digestion amount of CF, NDF, and ADF (p < 0.05), the serum concentrations of TC, TG, LDL-c, BUN, and MDA (p < 0.05), the relative abundance of Streptococcaceae (p < 0.05), and the abundance of PWY-6470, PWY0-862, HSERMETANA-PWY, LACTOSECAT-PWY, MET-SAM-PWY, PWY-6700, PWY-5347, PWY0-1061, and LACTOSECAT-PWY pathways (p < 0.05). The high-fiber diet increased average daily feed intake (p < 0.05), the serum concentrations of TC, TG, LDL-c, BUN, and MDA (p < 0.05), the relative abundance of Clostridiaceae_Clostridium and Coprococcus (p < 0.05), and the abundance of TCA-GLYOX-BYPASS, GLYCOLYSIS-TCA-GLYOX-BYPASS, and PWY-6906 pathways (p < 0.05). The high-fiber diet reduced chest circumference (p < 0.05) and ATTD of ether extract (EE), CF, NDF, ADF, Ca, CP, 18 amino acids (Trp, Thr, Val, Met, Ile, Leu, Phe, Lys, His, Arg Asp, Ser, Glu, Gly, Ala, Cys, Tyr, and Pro), EAA, NEAA, and TAA (p < 0.05). The high-fiber diet also reduced the serum concentrations of HDL-c, TP, ABL, and GLU, the serum enzyme activities of T-AOC, GSH-Px, SOD, and CAT (p < 0.05), and the relative abundance of Akkermansia and Oscillospira (p < 0.05). There was no significant effect of the interaction between multi-bacteria fermentation and dietary CF levels, except on the digestion amount of CF (p < 0.05). The 7.00% CF had a negative effect on the digestion of nutrients, but multi-bacteria solid-state fermentation diets could relieve this negative effect and increase backfat thickness. High-fiber diets and multi-bacteria solid-state fermentation improved the diversity and abundance of fecal microorganisms in finishing pigs.

[1]  Daniel A. Jacobo-Velázquez,et al.  Solid-state fermentation for enhancing the nutraceutical content of agrifood by-products: Recent advances and its industrial feasibility , 2021 .

[2]  Y. Duan,et al.  Effects of Dietary Isomaltooligosaccharide Levels on the Gut Microbiota, Immune Function of Sows, and the Diarrhea Rate of Their Offspring , 2021, Frontiers in Microbiology.

[3]  O. Adeola,et al.  Regression-Derived Ileal Endogenous Amino Acid Losses in Broiler Chickens and Cannulated Pigs Fed Corn Fiber, Wheat Bran, and Pectin , 2020, Animals : an open access journal from MDPI.

[4]  Bin Wu,et al.  Immobilization of the extracellular recombinant Lucky9 xylanase from Bacillus subtilis enhances activity at high temperature and pH , 2020, FEBS open bio.

[5]  X. Kong,et al.  Effect of fermented feed on growth performance, holistic metabolism and fecal microbiota in weanling piglets , 2020 .

[6]  A. Ariff,et al.  Enhancement of β-Mannanase Production by Bacillus subtilis ATCC11774 through Optimization of Medium Composition , 2020, Molecules.

[7]  Chong Zhang,et al.  Effect of solid state fermentation by Enterococcus faecalis M2 on antioxidant and nutritional properties of wheat bran , 2020 .

[8]  Eric J. C. Gálvez,et al.  Intestinal Microbiota of Fattening Pigs Offered Non-Fermented and Fermented Liquid Feed with and without the Supplementation of Non-Fermented Coarse Cereals , 2020, Microorganisms.

[9]  H. Stein,et al.  Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs , 2020, Journal of animal science.

[10]  Huawei Zeng,et al.  Advanced liver steatosis accompanies an increase in hepatic inflammation, colonic, secondary bile acids and Lactobacillaceae/Lachnospiraceae bacteria in C57BL/6 mice fed a high-fat diet. , 2020, The Journal of nutritional biochemistry.

[11]  A. Il’ina,et al.  Chitin/Chitosan and Its Derivatives: Fundamental Problems and Practical Approaches , 2020, Biochemistry (Moscow).

[12]  Ľ. Grešáková,et al.  The effect of organic and inorganic zinc source, used in combination with potato fiber, on growth, nutrient digestibility and biochemical blood profile in growing pigs , 2019, Livestock Science.

[13]  Adrijana Skugor,et al.  Candida utilis yeast as a protein source for weaned piglets: Effects on growth performance and digestive function , 2019, Livestock Science.

[14]  N. Ames,et al.  Flaxseed meal and oat hulls supplementation: impact on dietary fiber digestibility, and flows of fatty acids and bile acids in growing pigs. , 2018, Journal of animal science.

[15]  K. Peters,et al.  Peptidoglycan. , 2019, Sub-cellular biochemistry.

[16]  C. Ashworth,et al.  The role of dietary fibre in pig production, with a particular emphasis on reproduction , 2018, Journal of Animal Science and Biotechnology.

[17]  P. Gérard,et al.  The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism , 2018, Microbiome.

[18]  Ping Liu,et al.  Effects of Oat Bran on Nutrient Digestibility, Intestinal Microbiota, and Inflammatory Responses in the Hindgut of Growing Pigs , 2018, International journal of molecular sciences.

[19]  R. Barrena,et al.  Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste , 2018, Sustainability.

[20]  J. Chiang,et al.  Bile Acid Metabolism in Liver Pathobiology. , 2018, Gene expression.

[21]  Xi Ma,et al.  Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model , 2017, Scientific Reports.

[22]  H. Nielsen,et al.  Oscillospira and related bacteria - From metagenomic species to metabolic features. , 2017, Environmental microbiology.

[23]  M. Øverland,et al.  Yeast derived from lignocellulosic biomass as a sustainable feed resource for use in aquaculture. , 2017, Journal of the science of food and agriculture.

[24]  D. Ojcius,et al.  Pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections , 2016, Mucosal Immunology.

[25]  W. Wu,et al.  Dietary fibers influence the intestinal SCFAs and plasma metabolites profiling in growing pigs. , 2016, Food & function.

[26]  Y. Wang,et al.  The effects of dietary supplementation of microencapsulated and the extract of seed on growth performance, immune functions, and serum biochemical parameters in broiler chickens. , 2016, Journal of animal science.

[27]  Liping Zhou,et al.  Effects of the dietary protein level on the microbial composition and metabolomic profile in the hindgut of the pig. , 2016, Anaerobe.

[28]  Q. Ge,et al.  Effects of acute intra-abdominal hypertension on multiple intestinal barrier functions in rats , 2016, Scientific Reports.

[29]  P. Hylemon,et al.  Consequences of bile salt biotransformations by intestinal bacteria , 2016, Gut microbes.

[30]  Y. Wang,et al.  The effects of dietary supplementation of microencapsulated Enterococcus faecalis and the extract of Camellia oleifera seed on growth performance , immune functions , and serum biochemical parameters in broiler chickens 1 , 2016 .

[31]  M. Blaut,et al.  Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice. , 2015, The Journal of nutritional biochemistry.

[32]  C. Greenhill.  Gut microbiota: Firmicutes and Bacteroidetes involved in insulin resistance by mediating levels of glucagon-like peptide 1 , 2015, Nature Reviews Endocrinology.

[33]  M. Dearing,et al.  Unique and shared responses of the gut microbiota to prolonged fasting: a comparative study across five classes of vertebrate hosts. , 2014, FEMS microbiology ecology.

[34]  H. Zhang,et al.  Effect of graded levels of fiber from alfalfa meal on intestinal nutrient and energy flow, and hindgut fermentation in growing pigs. , 2013, Journal of animal science.

[35]  Barbara M. Bakker,et al.  The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism , 2013, Journal of Lipid Research.

[36]  M. Cappai,et al.  Electron microscopy and composition of raw acorn starch in relation to in vivo starch digestibility. , 2013, Food & function.

[37]  G. Jia,et al.  Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism. , 2013, Genetics and molecular research : GMR.

[38]  Defa Li,et al.  The effects of dietary fiber level on nutrient digestibility in growing pigs , 2013, Journal of Animal Science and Biotechnology.

[39]  S. Tims,et al.  Microbiota conservation and BMI signatures in adult monozygotic twins , 2012, The ISME Journal.

[40]  H. Chirakkal,et al.  Bacillus subtilis PB6 improves intestinal health of broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis. , 2013, Poultry science.

[41]  V. Fellner,et al.  Stabilized rice bran improves weaning pig performance via a prebiotic mechanism. , 2013, Journal of animal science.

[42]  L. Cosgrove,et al.  A review of the potential mechanisms for the lowering of colorectal oncogenesis by butyrate , 2012, British Journal of Nutrition.

[43]  P. Shinde,et al.  Evaluation of multi-microbe probiotics prepared by submerged liquid or solid substrate fermentation and antibiotics in weaning pigs , 2011 .

[44]  G. Michel,et al.  Environmental and Gut Bacteroidetes: The Food Connection , 2011, Front. Microbio..

[45]  M. Hattori,et al.  Bifidobacteria can protect from enteropathogenic infection through production of acetate , 2011, Nature.

[46]  G. Baeverfjord,et al.  Effects of dietary yeast cell wall β-glucans and MOS on performance, gut health, and salmon lice resistance in Atlantic salmon (Salmo salar) fed sunflower and soybean meal , 2010 .

[47]  T. Misztal,et al.  Physiological effects of long-term feeding diets supplemented with potato fibre or cellulose to adult rats , 2010, Archives of animal nutrition.

[48]  Y. Wang,et al.  Effect of dietary probiotic, Bacillus coagulans, on growth performance, chemical composition, and meat quality of Guangxi Yellow chicken. , 2010, Poultry science.

[49]  A. Pegg.  Mammalian polyamine metabolism and function , 2009, IUBMB life.

[50]  R. Tang,et al.  Real-time PCR quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs. , 2008, Anaerobe.

[51]  D. Jonkers,et al.  Review article: the role of butyrate on colonic function , 2007, Alimentary pharmacology & therapeutics.

[52]  J. Noblet,et al.  Influence of dietary fibre on digestive utilization and rate of passage in growing pigs, finishing pigs and adult sows , 2002 .

[53]  E. W. Crampton,et al.  The use of chromic oxide as an index for determining the digestibility of a diet. , 1950, The Journal of nutrition.