Fabrication of Plasmonic Nanorod-Embedded Dipeptide Microspheres via the Freeze-Quenching Method for Near-Infrared Laser-Triggered Drug-Delivery Applications.

Control of drug release by an external stimulus may provide remote controllability, low toxicity, and reduced side effects. In this context, varying physical external stimuli, including magnetic and electric fields, ultrasound, light, and pharmacological stimuli, have been employed to control the release rate of drug molecules in a diseased region. However, the design and development of alternative on-demand drug-delivery systems that permit control of the dosage of drug released via an external stimulus are still required. Here, we developed near-infrared laser-activatable microspheres based on Fmoc-diphenylalanine (Phe-Phe) dipeptides and plasmonic gold nanorods (AuNRs) via a simple freeze-quenching approach. These plasmonic nanoparticle-embedded microspheres were then employed as a smart drug-delivery platform for native, continuous, and pulsatile doxorubicin (DOX) release. Remarkable sustained, burst, and on-demand DOX release from the fabricated microspheres were achieved by manipulating the laser exposure time. Our results demonstrate that AuNR-embedded dipeptide microspheres have great potential for controlled drug-delivery systems.

[1]  S. Stupp,et al.  Induction of cancer cell death by self-assembling nanostructures incorporating a cytotoxic peptide. , 2010, Cancer research.

[2]  Renliang Huang,et al.  Solvent and surface controlled self-assembly of diphenylalanine peptide: from microtubes to nanofibers , 2011 .

[3]  Michael S. Goldberg,et al.  Nanostructured materials for applications in drug delivery and tissue engineering , 2007, Journal of biomaterials science. Polymer edition.

[4]  Jun Wang,et al.  Protein-based nanomedicine platforms for drug delivery. , 2009, Small.

[5]  A. Miller,et al.  Nanostructured Hydrogels for Three‐Dimensional Cell Culture Through Self‐Assembly of Fluorenylmethoxycarbonyl–Dipeptides , 2006 .

[6]  M. El-Sayed,et al.  Shape and size dependence of radiative, non-radiative and photothermal properties of gold nanocrystals , 2000 .

[7]  Mehmet Yilmaz,et al.  Combining 3-D plasmonic gold nanorod arrays with colloidal nanoparticles as a versatile concept for reliable, sensitive, and selective molecular detection by SERS. , 2014, Physical chemistry chemical physics : PCCP.

[8]  J. Karp,et al.  Nanocarriers as an Emerging Platform for Cancer Therapy , 2022 .

[9]  S. Kim,et al.  Morphology control of one-dimensional peptide nanostructures. , 2008, Journal of nanoscience and nanotechnology.

[10]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[11]  Job Boekhoven,et al.  A self-assembled delivery platform with post-production tunable release rate. , 2012, Journal of the American Chemical Society.

[12]  Meital Reches,et al.  Casting Metal Nanowires Within Discrete Self-Assembled Peptide Nanotubes , 2003, Science.

[13]  J. Fei,et al.  A peony-flower-like hierarchical mesocrystal formed by diphenylalanine , 2010 .

[14]  M. Demirel,et al.  Control of protein adsorption onto core-shell tubular and vesicular structures of diphenylalanine/parylene. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[15]  X. Zhu,et al.  Polymer microspheres for controlled drug release. , 2004, International journal of pharmaceutics.

[16]  A. Caflisch,et al.  Phenylalanine assembly into toxic fibrils suggests amyloid etiology in phenylketonuria. , 2012, Nature chemical biology.

[17]  J. Bandekar,et al.  Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins. , 1986, Advances in protein chemistry.

[18]  Meital Reches,et al.  Rigid, Self‐Assembled Hydrogel Composed of a Modified Aromatic Dipeptide , 2006 .

[19]  D. Seliktar,et al.  Self-assembled Fmoc-peptides as a platform for the formation of nanostructures and hydrogels. , 2009, Biomacromolecules.

[20]  Wim E Hennink,et al.  Biomedical Applications of Self-Assembling Peptides. , 2016, Bioconjugate chemistry.

[21]  M. Morris,et al.  Nanoporous polymeric nanofibers based on selectively etched PS-b-PDMS block copolymers. , 2012, ACS applied materials & interfaces.

[22]  Junbai Li,et al.  Organogels Based on Self-Assembly of Diphenylalanine Peptide and Their Application To Immobilize Quantum Dots , 2008 .

[23]  M. Yilmaz,et al.  Light‐Driven Unidirectional Liquid Motion on Anisotropic Gold Nanorod Arrays , 2015 .

[24]  Hong Chi,et al.  A simple, reliable and sensitive colorimetric visualization of melamine in milk by unmodified gold nanoparticles. , 2010, The Analyst.

[25]  Sonke Svenson,et al.  Dendrimers as versatile platform in drug delivery applications. , 2009, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[26]  S. Glotzer,et al.  The effect of nanometre-scale structure on interfacial energy. , 2009, Nature materials.

[27]  M. Reches,et al.  Coassembly of aromatic dipeptides into biomolecular necklaces. , 2012, ACS nano.

[28]  Anda Vintiloiu,et al.  Organogels and their use in drug delivery--a review. , 2008, Journal of controlled release : official journal of the Controlled Release Society.

[29]  G. Demirel,et al.  Laser-triggered degelation control of gold nanoparticle embedded peptide organogels. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[30]  Ali Khademhosseini,et al.  Development of functional biomaterials with micro‐ and nanoscale technologies for tissue engineering and drug delivery applications , 2014, Journal of tissue engineering and regenerative medicine.

[31]  Kostas Kostarelos,et al.  Liposomes: from a clinically established drug delivery system to a nanoparticle platform for theranostic nanomedicine. , 2011, Accounts of chemical research.