Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies

Microbial lipid is a potential alternative feedstock for the biodiesel industry. New culture strategies remain to be developed to improve the economics of microbial lipid technology. This work describes lipid production by the oleaginous yeast Rhodosporidium toruloides Y4 using a 15-l bioreactor with different substrate feeding strategies. Among these strategies, the intermittent feeding mode gave a lipid productivity of 0.36 g l−1 h−1, whereas the constant glucose concentration II (CC-II) mode gave the highest lipid productivity of 0.57 g l−1 h−1. The repeated fed-batch mode according to the CC-II mode was performed with a duration time of 358 h, and the overall lipid productivity was 0.55 g l−1 h−1. Our results suggested that substrate feeding modes had a great impact on lipid productivity and that the repeated fed-batch process was the most appealing method by which to enhance microbial lipid production.

[1]  F. Bai,et al.  [Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides]. , 2006, Sheng wu gong cheng xue bao = Chinese journal of biotechnology.

[2]  Jin-Ho Seo,et al.  Scale-up of erythritol production by an osmophilic mutant of Candida magnoliae , 2003, Biotechnology Letters.

[3]  Hui Luo,et al.  Studies on lipid production by Rhodotorula glutinis fermentation using monosodium glutamate wastewater as culture medium. , 2008, Bioresource technology.

[4]  T. Yano,et al.  Optimum substrate feed rate in fed-batch culture with the DO-stat method , 1991 .

[5]  C. Ratledge,et al.  The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. , 2002, Advances in applied microbiology.

[6]  Bin Lai,et al.  A comparative study on four method of fungi lipid extraction , 2001 .

[7]  C. T. Evans,et al.  Influence of Nitrogen Metabolism on Lipid Accumulation by Rhodosporidium toruloides CBS 14 , 1984 .

[8]  Bo Liu,et al.  Optimization of Culture Conditions for Lipid Production by Rhodosporidium toruloides , 2006 .

[9]  Xin Zhao,et al.  Effects of biomass hydrolysis by-products on oleaginous yeast Rhodosporidium toruloides. , 2009, Bioresource technology.

[10]  Fengwu Bai,et al.  High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture , 2007 .

[11]  B. Kristiansen,et al.  Substrate inhibition kinetics of Saccharomyces cerevisiae in fed-batch cultures operated at constant glucose and maltose concentration levels , 2007, Journal of Industrial Microbiology & Biotechnology.

[12]  S. Papanikolaou,et al.  Lipids of Cunninghamella echinulata with emphasis to γ-linolenic acid distribution among lipid classes , 2006, Applied Microbiology and Biotechnology.

[13]  Hiroaki Yamauchi,et al.  Mass production of lipids by Lipomyces starkeyi in microcomputer-aided fed-batch culture. , 1983 .

[14]  Tsuneo Yamane,et al.  Mass production of poly-β-hydroxybutyric acid by fed-batch culture with controlled carbon/nitrogen feeding , 1986, Applied Microbiology and Biotechnology.

[15]  M. Shimoda,et al.  Optimization of L-lactic acid feeding for the production of poly-D-3-hydroxybutyric acid by Alcaligenes eutrophus in fed-batch culture. , 1999, Journal of bioscience and bioengineering.

[16]  Q. Wang,et al.  Lipid production from Jerusalem artichoke by Rhodosporidium toruloides Y4 , 2010, Journal of Industrial Microbiology & Biotechnology.

[17]  J. Rhee,et al.  High density cell culture ofRhodotorulaglutinis using oxygen-enriched air , 1986, Biotechnology Letters.

[18]  C. T. Evans,et al.  A comparison of the oleaginous yeast,Candida curvata, grown on different carbon sources in continuous and batch culture , 1983, Lipids.

[19]  C. Ratledge.  Lipid biotechnology: A wonderland for the microbial physiologist , 1987 .

[20]  Paul F. Greenfield,et al.  Optimal feeding policy for recombinant protein production via the yeast saccharomyces cerevisiae in fed-batch culture , 1994 .

[21]  Z. Zhao,et al.  Biodiesel production by direct methanolysis of oleaginous microbial biomass , 2007 .

[22]  Mo Xian,et al.  Biodiesel production from oleaginous microorganisms , 2009 .

[23]  H. Chang,et al.  Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations , 2003, Biotechnology Letters.

[24]  Takeshi Kobayashi,et al.  Insecticide production by recombinant Bacillus subtilis 1A96 in fed-batch culture with control of glucose concentration , 1993 .

[25]  G. Goma,et al.  Lipid production by an unsaturated fatty acid auxotroph of the oleaginous yeast Apiotrichum curvatum grown in single-stage continuous culture , 1993, Applied Microbiology and Biotechnology.

[26]  T. Hermann.  Industrial production of amino acids by coryneform bacteria. , 2003, Journal of biotechnology.

[27]  F. Wagner,et al.  Influence of substrate supply on the production of sophorose lipids by Candida bombicola ATCC 22214 , 1996, Biotechnology Letters.