A preliminary study of the development of 19S and 7S immunoglobulins in sera of two sheep vaccinated with Brucella abortus, strain 19.

The pattern of antibody response to vaccination with Brucella abortus, strain 19, was studied in two sheep. Agglutinative activity was detected by the third and fifth days and complement - fixing activity by the fifth and seventh days post-vaccination. Density gradient centrifugation and DEAE cellulose column chromatography showed the 19S antibody developed first, followed soon after by 7S antibody. The former had disappeared by the 25th day but the latter persisted longer in both sheep. A small amount of 19S antibody was detected in sheep 1 following a booster dose of vaccine but 7S antibody constituted the major secondary response. The standard tube agglutination test was found to be more efficient than the complement-fixation test for titration of 19S antibody. An increase in the salt concentration to 10% in tube agglutination test rendered it more sensitive in demonstrating 7S antibody.

[1]  C. E. Rice,et al.  Antibody development in pigs inoculated with live or killed cultures of Brucella abortus. , 1968, Canadian journal of comparative medicine : Revue canadienne de medecine comparee.

[2]  C. E. Rice,et al.  Chromatographic studies of sera from calves vaccinated with brucella abortus, strain 19. , 1967, Canadian journal of comparative medicine and veterinary science.

[3]  C. E. Rice,et al.  Ultracentrifugal studies of sera from cattle vaccinated or naturally infected with Brucella abortus. , 1966, Canadian journal of comparative medicine and veterinary science.

[4]  C. E. Rice,et al.  Electrophoretic studies of sera from cattle vaccinated or naturally infected with Brucella abortus. , 1966, Canadian journal of comparative medicine and veterinary science.

[5]  A. G. Osler,et al.  Weight estimates of rabbit anti-human serum albumin based on antigen-binding and precipitin analyses: specific hemagglutinating activities of 7 S and 19 S components. , 1966, Journal of immunology.

[6]  A. A. Benedict.  Sensitivity of Passive Haemagglutination for Assay of 7S and 19S Antibodies in Primary Rabbit Anti-bovine Serum Albumin Sera , 1965, Nature.

[7]  E. S. Orlov.  Studies on pathogenesis, diagnosis and specific prophylaxis of swine brucellosis. , 1965, Bulletin - Office international des epizooties.

[8]  K. P. Studentsov.  Some questions of diagnosis of brucellosis and specific preventive measures in the Kazakh Soviet Republic. , 1965, Bulletin - Office international des epizooties.

[9]  G. V. van Waveren.  The value of the complement fixation test for the eradication of bovine brucellosis in the Netherlands. , 1965, Bulletin - Office international des epizooties.

[10]  J. E. Rose,et al.  ULTRACENTRIFUGATION AND HEAT-INACTIVATION STUDIES ON SEROAGGLUTININS OF PREGNANT HEIFERS ARTIFICIALLY INFECTED WITH VIRULENT BRUCELLA ABORTUS. , 1964, American journal of veterinary research.

[11]  D. Moore,et al.  REACTION OF 7S AND 19S COMPONENTS OF IMMUNE RABBIT ANTISERA WITH HUMAN GROUP A AND AB RED CELLS. , 1963, Immunology.

[12]  B. Benacerraf,et al.  PROPERTIES OF GUINEA PIG 7S ANTIBODIES III. IDENTIFICATION OF ANTIBODIES INVOLVED IN COMPLEMENT FIXATION AND HEMOLYSIS , 1963 .

[13]  J. Porath,et al.  Gel filtration of proteins, peptides and amino acids. , 1960, Biochimica et biophysica acta.

[14]  H. A. Sober,et al.  Protein chromatography on ion exchange cellulose. , 1958, Federation proceedings.

[15]  M. K. Brakke.  Zonal separations by density-gradient centrifugation. , 1953, Archives of biochemistry and biophysics.