Abstract The future of personalized diagnostics commences on the single cell level. Even high-end technologies like Next Generation Sequencing can be improved if applied on pure single cell populations (e.g., tumor cells without contaminating stromal cells) or on a single cell level (DNA/RNA sequencing). The vast majority of these technologies need individual and preferably undistorted cells for the analytical process. Thus, decisive prerequisite for high-end analytics is to remove cells from their tissue matrix as gently as possible. This can be accomplished by an enzyme-free, fast and reproducible approach of generating pure and individual single cells from tissue samples. In this study we demonstrate the utility of a semi-automated Tissue Grinder that is compatible with standard 50 ml centrifuge tubes and standard cell strainer for mechanically, nonenzymatic and parallel processing of tissue samples. We show that without enzymatic treatment viable single-cell yields match or exceed reference enzymatic methods, while reducing processing time by at least 80%.
[1]
Elliot E Hui,et al.
Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells.
,
2018,
Lab on a chip.
[2]
Jin‐Ming Lin,et al.
Microfluidic Devices in the Fast-Growing Domain of Single-Cell Analysis.
,
2018,
Chemistry.
[3]
A. Jager,et al.
Ex vivo tumor culture systems for functional drug testing and therapy response prediction
,
2017,
Future science OA.
[4]
Roland Zengerle,et al.
Technologies for Single-Cell Isolation
,
2015,
International journal of molecular sciences.
[5]
Boon Chong Tan,et al.
Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method
,
2011,
Cell biology international.
[6]
Robert Schmitt,et al.
Parallelization in Automated Stem Cell Culture
,
2017
.
[7]
Andre Sharon,et al.
Automated Tissue Dissociation for Rapid Extraction of Viable Cells
,
2017
.