Activation of restriction enzyme by electrochemically released magnesium ion.

Observation and cutting of DNA molecules at intended positions permit several new experimental methods that are completely different from conventional molecular biology methods; therefore several cutting methods have been proposed and studied. In this paper, a new cutting method for a DNA molecule by localizing the activity of a restriction enzyme is presented. Since most restriction enzymes require magnesium ions for their activation, local restriction enzyme activity can be controlled by the local concentration of magnesium ions. Applying a direct current (dc) voltage to a needle electrode of metallic magnesium made it possible to control the local magnesium ion concentration at the tip of the needle. The restriction enzyme was activated only when magnesium ions were electrochemically supplied.

[1]  Shinji Katsura,et al.  Micro manipulation of cells and DNA molecules , 1995 .

[2]  A Bensimon,et al.  Alignment and sensitive detection of DNA by a moving interface. , 1994, Science.

[3]  Takatoki Yamamoto,et al.  Moleculer Surgery of DNA Using Enzyme-Immobilized Particles , 1996 .

[4]  Shinji Katsura,et al.  Handling of single DNA molecule using electric field and laser beam , 1994, Proceedings of 1994 IEEE Industry Applications Society Annual Meeting.

[5]  B. Zimm.  Extension in Flow of a DNA Molecule Tethered at One End , 1998 .

[6]  Masao Washizu,et al.  Applications of electrostatic stretch-and-positioning of DNA , 1993, Conference Record of the 1993 IEEE Industry Applications Conference Twenty-Eighth IAS Annual Meeting.

[7]  K. Greulich,et al.  Laser manipulation and UV induced single molecule reactions of individual DNA molecules , 1996 .

[8]  M. Washizu,et al.  Electrostatic manipulation of DNA in microfabricated structures , 1989, Conference Record of the IEEE Industry Applications Society Annual Meeting,.

[9]  B. Trask,et al.  A new method for straightening DNA molecules for optical restriction mapping. , 1997, Nucleic acids research.