Physiological Response of Nutrient-Stressed Lemna gibba to Pulse Colloidal Silver Treatment

Wastewater is a source of many environmental pollutants and potentially high concentrations of essential plant nutrients. Site-specific nutrient levels may influence the response of exposed plants to a chemical stressor. In the present study, we focused on the responses of model aquatic macrophyte swollen duckweed (Lemna gibba L.) to a short pulse exposure and a commercially available colloidal silver product as a potential environmental chemical stressor, combined with two levels of total nitrogen and phosphorus nutrition. Treatment with the commercially available colloidal silver product caused oxidative stress in L. gibba plants under both high and low nutrient levels. Plants grown and treated under high nutrient levels showed lower levels of lipid peroxidation and hydrogen peroxide accumulation, as well as higher levels of photosynthetic pigment content in comparison to treated plants under low nutrient levels. Higher free radical scavenging activity for plants treated with silver in combination with high nutrient levels resulted in better overall protection from silver-induced oxidative stress. The results showed that external nutrient levels significantly affected the L. gibba plant’s response to the colloidal silver presence in the environment and that nutrient levels should be considered in the assessment of potential environmental impact for contaminants.

[1]  G. Kalčíková,et al.  The Response of Duckweed Lemna minor to Microplastics and Its Potential Use as a Bioindicator of Microplastic Pollution , 2022, Plants.

[2]  Gaojie Li,et al.  Combined effects of temperature and nutrients on the toxicity of cadmium in duckweed (Lemna aequinoctialis). , 2022, Journal of hazardous materials.

[3]  Yajun Wang,et al.  Impact of microplastics on the foraging, photosynthesis and digestive systems of submerged carnivorous macrophytes under low and high nutrient concentrations. , 2021, Environmental pollution.

[4]  Raluca M. Fratila,et al.  Fate and transformation of silver nanoparticles in different biological conditions , 2021, Beilstein journal of nanotechnology.

[5]  S. Ceschin,et al.  Phytoremediation potential of the duckweeds Lemna minuta and Lemna minor to remove nutrients from treated waters , 2020, Environmental Science and Pollution Research.

[6]  Shakeel Ahmad Dar,et al.  Concerns and Threats of Contamination on Aquatic Ecosystems , 2020, Bioremediation and Biotechnology.

[7]  A. Banaszak,et al.  Anthropogenic pollution of aquatic ecosystems: Emerging problems with global implications. , 2020, The Science of the total environment.

[8]  Jin-Hee Lim,et al.  Detection and Characterization of Silver Nanostructures in Consumer Products. , 2019, Journal of Nanoscience and Nanotechnology.

[9]  R. Boukherroub,et al.  Toxicity Effect of Silver Nanoparticles on Photosynthetic Pigment Content, Growth, ROS Production and Ultrastructural Changes of Microalgae Chlorella vulgaris , 2019, Nanomaterials.

[10]  T. Lamaze,et al.  Environmental variations mediate duckweed (Lemna minor L.) sensitivity to copper exposure through phenotypic plasticity , 2019, Environmental Science and Pollution Research.

[11]  Z. Lončarić,et al.  Physiological and biochemical effect of silver on the aquatic plant Lemna gibba L.: Evaluation of commercially available product containing colloidal silver. , 2019, Aquatic toxicology.

[12]  L. Yao,et al.  Fate and toxicity of silver nanoparticles in freshwater from laboratory to realistic environments: a review , 2019, Environmental Science and Pollution Research.

[13]  Brad M. Angel,et al.  The use of time-averaged concentrations of metals to predict the toxicity of pulsed complex effluent exposures to a freshwater alga. , 2018, Environmental pollution.

[14]  J. Navrátilová,et al.  Characterization of engineered nanoparticles in commercially available spray disinfectant products advertised to contain colloidal silver. , 2018, The Science of the total environment.

[15]  Benjamin P Colman,et al.  Stress Responses of Aquatic Plants to Silver Nanoparticles. , 2018, Environmental science & technology.

[16]  S. Mohsenzadeh,et al.  Differential Expression of Mitochondrial Manganese Superoxide Dismutase (SOD) in Triticum aestivum Exposed to Silver Nitrate and Silver Nanoparticles. , 2017, Iranian journal of biotechnology.

[17]  Brad M. Angel,et al.  Time-averaged concentrations are effective for predicting chronic toxicity of varying copper pulse exposures for two freshwater green algae species. , 2017, Environmental pollution.

[18]  S. Sahi,et al.  Differential phytotoxic responses of silver nitrate (AgNO3) and silver nanoparticle (AgNps) in Cucumis sativus L. , 2017 .

[19]  Madhulika Singh,et al.  Nitrogen modifies NaCl toxicity in eggplant seedlings: Assessment of chlorophyll a fluorescence, antioxidative response and proline metabolism , 2016 .

[20]  Z. Lončarić,et al.  Response of Lemna minor L. to short-term cobalt exposure: The effect on photosynthetic electron transport chain and induction of oxidative damage. , 2016, Aquatic toxicology.

[21]  J. Stillman,et al.  Multiple Stressors in a Changing World: The Need for an Improved Perspective on Physiological Responses to the Dynamic Marine Environment. , 2016, Annual review of marine science.

[22]  H. Vandenhove,et al.  The first draft genome of the aquatic model plant Lemna minor opens the route for future stress physiology research and biotechnological applications , 2015, Biotechnology for Biofuels.

[23]  Rawiwan Maniratanachote,et al.  Determination of silver in personal care nanoproducts and effects on dermal exposure , 2015, Journal of Nanoparticle Research.

[24]  H. Segner,et al.  Molecular and cellular effects of contamination in aquatic ecosystems , 2015, Environmental Science and Pollution Research.

[25]  F. Rossi,et al.  Detection, quantification and derivation of number size distribution of silver nanoparticles in antimicrobial consumer products , 2015 .

[26]  G. Bystrzejewska-Piotrowska,et al.  Toxic Effect of Silver and Platinum Nanoparticles Toward the Freshwater Microalga Pseudokirchneriella subcapitata , 2015, Bulletin of Environmental Contamination and Toxicology.

[27]  S. Sarkar,et al.  Phytoremediation Potential of Duckweed (Lemna minor L.) On Steel Wastewater , 2015, International journal of phytoremediation.

[28]  B. S. Esteves,et al.  Short-term physiological responses to copper stress in Salvinia auriculata Aubl. , 2014 .

[29]  H. Jiang,et al.  Silver nanoparticles induced accumulation of reactive oxygen species and alteration of antioxidant systems in the aquatic plant Spirodela polyrhiza , 2014, Environmental toxicology and chemistry.

[30]  Changwei Hu,et al.  Biochemical Responses of Duckweed (Spirodela polyrhiza) to Zinc Oxide Nanoparticles , 2013, Archives of Environmental Contamination and Toxicology.

[31]  F. Rubio,et al.  Potassium starvation induces oxidative stress in Solanum lycopersicum L. roots. , 2012, Journal of plant physiology.

[32]  Wei Li,et al.  Physiological analysis of silver nanoparticles and AgNO3 toxicity to Spirodela polyrhiza , 2012, Environmental toxicology and chemistry.

[33]  A. Khataee,et al.  Phytoremediation potential of duckweed (Lemna minor L.) in degradation of C.I. Acid Blue 92: artificial neural network modeling. , 2012, Ecotoxicology and environmental safety.

[34]  C. Krishnaraj,et al.  Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. plant growth metabolism , 2012 .

[35]  J. Belgers,et al.  Can time-weighted average concentrations be used to assess the risks of metsulfuron-methyl to Myriophyllum spicatum under different time-variable exposure regimes? , 2011, Chemosphere.

[36]  Y. Tsay,et al.  Nitrate, ammonium, and potassium sensing and signaling. , 2010, Current opinion in plant biology.

[37]  W. Xing,et al.  Antioxidative responses of Elodea nuttallii (Planch.) H. St. John to short-term iron exposure. , 2010, Plant physiology and biochemistry : PPB.

[38]  A. Aksoy,et al.  Growth and Lead Accumulation Capacity of Lemna minor and Spirodela polyrhiza (Lemnaceae): Interactions with Nutrient Enrichment , 2010, Water, air, and soil pollution.

[39]  C. Lillo,et al.  Synergetic effects of nitrogen depletion, temperature, and light on the content of phenolic compounds and gene expression in leaves of tomato. , 2010, Phytochemistry.

[40]  D. Schachtman,et al.  A peroxidase contributes to ROS production during Arabidopsis root response to potassium deficiency. , 2010, Molecular plant.

[41]  H. Yang,et al.  Accumulation and acute toxicity of silver in Potamogeton crispus L. , 2010, Journal of hazardous materials.

[42]  R. Brain,et al.  Influence of nitrogen and phosphorus concentrations and ratios on Lemna gibba growth responses to triclosan in laboratory and stream mesocosm experiments , 2009, Environmental toxicology and chemistry.

[43]  R. Scholz,et al.  Modeled environmental concentrations of engineered nanomaterials (TiO(2), ZnO, Ag, CNT, Fullerenes) for different regions. , 2009, Environmental science & technology.

[44]  Camille Bénard,et al.  The 'trade-off' between synthesis of primary and secondary compounds in young tomato leaves is altered by nitrate nutrition: experimental evidence and model consistency. , 2009, Journal of experimental botany.

[45]  C. Lillo.  Signalling cascades integrating light-enhanced nitrate metabolism. , 2008, The Biochemical journal.

[46]  Emily D. Niemeyer,et al.  Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of basil (Ocimum basilicum L.). , 2008, Journal of agricultural and food chemistry.

[47]  J. Razinger,et al.  Oxidative stress in duckweed (Lemna minor L.) caused by short-term cadmium exposure. , 2008, Environmental pollution.

[48]  L. Drinovec,et al.  Antioxidative responses of duckweed (Lemna minor L.) to short-term copper exposure , 2007, Environmental science and pollution research international.

[49]  M. Sökmen,et al.  Changes in antioxidant activity, total phenolic and abscisic acid constituents in the aquatic plants Myriophyllum spicatum L. and Myriophyllum triphyllum Orchard exposed to cadmium , 2007, Ecotoxicology.

[50]  R. Tewari,et al.  Oxidative Stress and Antioxidant Responses in Young Leaves of Mulberry Plants Grown Under Nitrogen, Phosphorus or Potassium Deficiency , 2007 .

[51]  D. Schachtman,et al.  Reactive oxygen species and root hairs in Arabidopsis root response to nitrogen, phosphorus and potassium deficiency. , 2005, Plant & cell physiology.

[52]  M. Greger,et al.  Influence of nutrient levels on uptake and effects of mercury, cadmium, and lead in water spinach. , 2004, Journal of environmental quality.

[53]  D. Schachtman,et al.  Hydrogen peroxide mediates plant root cell response to nutrient deprivation. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[54]  S. Verma,et al.  Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants , 2003 .

[55]  E. Laurenti,et al.  Free radical production in roots of Phaseolus vulgaris subjected to phosphate deficiency stress , 2002 .

[56]  I. Graham,et al.  The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissues , 2001 .

[57]  G. D. Lemon,et al.  Potential and realized rates of vegetative reproduction in Spirodela polyrhiza, Lemna minor, and Wolffia borealis , 2001 .

[58]  W. Waller,et al.  Field assessments in conjunction with whole effluent toxicity testing , 2000 .

[59]  M. Choudhuri,et al.  Implications of water stress‐induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings , 1983 .

[60]  K. Asada,et al.  Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts , 1981 .

[61]  C. N. Giannopolitis,et al.  Superoxide dismutases: I. Occurrence in higher plants. , 1977, Plant physiology.

[62]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[63]  W B Jakoby,et al.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. , 1974, The Journal of biological chemistry.

[64]  A. Galston,et al.  The isoperoxidases of Pisum sativum. , 1967, Plant physiology.

[65]  F. Seidel,et al.  Zell- und stoffwechselphysiologische Untersuchungen an der Wurzel von Lemna minor L. unter besonderer Berücksichtigung von Kalium- und Kalziummangel , 1950, Planta.

[66]  P. S. Reddy,et al.  Plant Signaling Molecules: Role and Regulation under Stressful Environments , 2019 .

[67]  Tao Fang,et al.  Chemical transformation of silver nanoparticles in aquatic environments: Mechanism, morphology and toxicity. , 2018, Chemosphere.

[68]  Ivna Štolfa,et al.  Heavy Metal-Induced Oxidative Stress in Plants: Response of the Antioxidative System , 2015 .

[69]  D. Gupta,et al.  Reactive Oxygen Species and Oxidative Damage in Plants Under Stress , 2015, Springer International Publishing.

[70]  Marija Marijanović Rajčić,et al.  Ecotoxicological assessment of industrial effluent using duckweed (Lemna minor L.) as a test organism , 2010, Ecotoxicology.

[71]  B. Zhang,et al.  Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. , 2008, Journal of integrative plant biology.

[72]  Qunhui Wang,et al.  Effects of copper and cadmium on heavy metal polluted waterbody restoration by duckweed (Lemna minor). , 2007, Plant physiology and biochemistry : PPB.

[73]  B. Tripathi,et al.  Oxidative stress in Scenedesmus sp. during short- and long-term exposure to Cu2+ and Zn2+. , 2006, Chemosphere.

[74]  A. Fernie,et al.  Metabolic profiling reveals altered nitrogen nutrient regimes have diverse effects on the metabolism of hydroponically-grown tomato (Solanum lycopersicum) plants , 2005 .

[75]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .

[76]  D. Dolphin,et al.  Glutathione : chemical, biochemical, and medical aspects , 1989 .

[77]  H. Lichtenthaler.  CHLOROPHYLL AND CAROTENOIDS: PIGMENTS OF PHOTOSYNTHETIC BIOMEMBRANES , 1987 .

[78]  H. Aebi,et al.  Catalase in vitro. , 1984, Methods in enzymology.

[79]  I. Fridovich,et al.  Superoxide dismutases. , 1975, Annual review of biochemistry.

[80]  V. L. Singleton,et al.  Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents , 1965, American Journal of Enology and Viticulture.