Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI.

Cloning by the transplantation of somatic nuclei into unfertilized eggs requires a dramatic remodeling of chromosomal architecture. Many proteins are specifically lost from nuclei, and others are taken up from the egg cytoplasm. Recreating this exchange in vitro, we identified the chromatin-remodeling nucleosomal adenosine triphosphatase (ATPase) ISWI as a key molecule in this process. ISWI actively erases the TATA binding protein from association with the nuclear matrix. Defining the biochemistry of global nuclear remodeling may facilitate the efficiency of cloning and other dedifferentiation events that establish new stem cell lineages.

[1]  A. Wolffe,et al.  ATP-Dependent histone octamer mobilization and histone deacetylation mediated by the Mi-2 chromatin remodeling complex. , 2000, Biochemistry.

[2]  Alan Colman,et al.  The future of cloning , 1999, Nature.

[3]  G. Veenstra,et al.  Translation of Maternal TATA-Binding Protein mRNA Potentiates Basal but Not Activated Transcription in Xenopus Embryos at the Midblastula Transition , 1999, Molecular and Cellular Biology.

[4]  H. Kimura,et al.  Quantitation of RNA Polymerase II and Its Transcription Factors in an HeLa Cell: Little Soluble Holoenzyme but Significant Amounts of Polymerases Attached to the Nuclear Substructure , 1999, Molecular and Cellular Biology.

[5]  Ali Hamiche,et al.  ATP-Dependent Histone Octamer Sliding Mediated by the Chromatin Remodeling Complex NURF , 1999, Cell.

[6]  J. Jeddeloh,et al.  Maintenance of genomic methylation requires a SWI2/SNF2-like protein , 1999, Nature Genetics.

[7]  G. Längst,et al.  ISWI is an ATP-dependent nucleosome remodeling factor. , 1999, Molecular cell.

[8]  Maurizio Zuccotti,et al.  Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei , 1998, Nature.

[9]  A. Wolffe,et al.  A multiple subunit Mi-2 histone deacetylase from Xenopus laevis cofractionates with an associated Snf2 superfamily ATPase , 1998, Current Biology.

[10]  B. Pugh,et al.  Cloning and Biochemical Characterization of TAF-172, a Human Homolog of Yeast Mot1 , 1998, Molecular and Cellular Biology.

[11]  M. Surani,et al.  Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells , 1997, The EMBO journal.

[12]  J. Weitzel,et al.  Chicken MAR-binding protein ARBP is homologous to rat methyl-CpG-binding protein MeCP2 , 1997, Molecular and cellular biology.

[13]  Matthias Mann,et al.  Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II , 1997, Nature.

[14]  Ryuji Kobayashi,et al.  ACF, an ISWI-Containing and ATP-Utilizing Chromatin Assembly and Remodeling Factor , 1997, Cell.

[15]  I. Wilmut,et al.  "Viable Offspring Derived from Fetal and Adult Mammalian Cells" (1997), by Ian Wilmut et al. , 2014 .

[16]  A. Wolffe,et al.  Remodeling somatic nuclei in Xenopus laevis egg extracts: molecular mechanisms for the selective release of histones H1 and H1(0) from chromatin and the acquisition of transcriptional competence. , 1996, The EMBO journal.

[17]  J. Davie,et al.  Properties of chicken erythrocyte histone deacetylase associated with the nuclear matrix. , 1996, The Biochemical journal.

[18]  Toshio Tsukiyama,et al.  ISWI, a member of the SWl2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor , 1995, Cell.

[19]  J. Thorner,et al.  Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism. , 1994, Genes & development.

[20]  N. Hernandez,et al.  TBP, a universal eukaryotic transcription factor? , 1993, Genes & development.

[21]  A. Philpott,et al.  Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts , 1992, Cell.

[22]  D. Newmeyer,et al.  In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins , 1986, The Journal of cell biology.

[23]  J. Gurdon,et al.  Adult frogs derived from the nuclei of single somatic cells. , 1962, Developmental biology.

[24]  E. Nanak.  Metal affinity electrophoresis: An analytical tool , 1992 .

[25]  S. Adam,et al.  Nuclear protein import using digitonin-permeabilized cells. , 1992, Methods in enzymology.

[26]  R. Chalkley,et al.  Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in vitro. , 1989, Methods in enzymology.