[A model of phenotypic plasticity: the hypothalamo-posthypophyseal neurons].

In neurons, which are post-mitotic cells, a structural and biochemical plasticity occurs, namely for the mediators. The magnocellular hypothalamic neurons innervating the neural lobe are a favourable model for the study of the dynamic aspects of neuropeptides expression. In fact, they synthetize these peptides in large amounts, as a function of the physiological or experimental conditions. In addition to the major neuropeptides, oxytocin (OT) and vasopressin (VP) which are contained in control rats within different neuronal populations, immunocytochemistry and in situ hybridization used alone or in combination in the same preparations have revealed other neuropeptides and corresponding mRNAs respectively. These multiple labellings demonstrate that after osmotic stimulation or during lactation some neurons are able to synthetize OT and VP simultaneously, together with galanin and even tyrosine hydroxylase (but not catecholamines). Similarly, hypophysectomy or transection of the pituitary stalk differentially modify the contents in mRNA coding for VP, OT and galanin. These results have also been described in other neuronal types such as spinal ganglia sensory neurons, suggesting possible mechanisms at different levels of genetic expression: transcription, translation, post-translational events and possibly interneuronal exchanges of mRNA. The in vivo regulation of this neurochemical plasticity probably involves the innervation of these neurons and perhaps the colocalized peptides themselves. In fact, galanin selectively inhibits the expression of VP but not that of OT. Functional implications of the neuronal phenotypic plasticity in the adaptation of the nervous system to the changing physiological conditions are discussed, together with its possible implications in pathology and therapeutics.