The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.

Mutations at the suppressor of Hairy-wing [su(Hw), 3-54.8] locus reverse the phenotype of second-site mutations induced by the gypsy transposable element in Drosophila melanogaster. This gene encodes a protein with a predicted molecular weight of 109,000 that contains an acidic domain and 12 copies of the DNA-binding 'Zn finger' motif. The su(Hw) protein was overexpressed in Escherichia coli and Drosophila cells, and partially purified. It was shown to interact specifically in vitro with a 367-bp DNA fragment that contains 12 copies of the sequence PyPuTTGCATACCPy located in the 5'-untranslated region of gypsy, between the 5' long terminal repeat (LTR) and the first ATG initiation codon. This sequence shows striking homology to some mammalian transcriptional enhancer elements, supporting a role for the su(Hw) protein in the control of gypsy transcription. In addition, the su(Hw) protein is present at approximately 100-200 sites on Drosophila polytene chromosomes, suggesting that it also interacts in vivo with DNA and might be involved functionally in the regulation of normal cellular genes.

[1]  S. Parkhurst,et al.  Retroviral elements and suppressor genes in Drosophila , 1986, BioEssays : news and reviews in molecular, cellular and developmental biology.

[2]  Aaron Klug,et al.  ‘Zinc fingers’: a novel protein motif for nucleic acid recognition , 1987 .

[3]  S. Parkhurst,et al.  The Drosophila melanogaster gypsy transposable element encodes putative gene products homologous to retroviral proteins. , 1986, Molecular and cellular biology.

[4]  Y. Ilyin,et al.  jockey, a mobile drosophila element similar to mammalian LINEs, is transcribed from the internal promoter by RNA polymerase II , 1988, Cell.

[5]  D. Brutlag,et al.  Multiple forms and cellular localization of Drosophila DNA topoisomerase II. , 1986, The Journal of biological chemistry.

[6]  G. Rubin.  CHAPTER 8 – Dispersed Repetitive DNAs in Drosophila , 1983 .

[7]  C. Dieckmann,et al.  Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b. , 1985, The Journal of biological chemistry.

[8]  Kevin Struhl,et al.  Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein , 1988, Nature.

[9]  D. Crothers,et al.  Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. , 1981, Nucleic acids research.

[10]  R. Kelley,et al.  The Drosophila su(Hw) gene, which controls the phenotypic effect of the gypsy transposable element, encodes a putative DNA-binding protein. , 1988, Genes & development.

[11]  A. McDonough.  Chapter 8 Use of Antibodies in the Study of Na+, K+-ATPase Biosynthesis and Structure , 1984 .

[12]  L. H. Cohen,et al.  Histones of polytene and nonpolytene nuclei of Drosophila melanogaster. , 1971, Journal of Biological Chemistry.

[13]  M. Churchill,et al.  Mapping functional regions of transcription factor TFIIIA , 1988, Molecular and cellular biology.

[14]  D. Thierry-Mieg,et al.  Genetic interactions of modifier genes and modifiable alleles in Drosophila melanogaster. , 1988, Genetics.

[15]  K. Moses,et al.  Molecular analysis of the yellow locus of Drosophila , 1986, The EMBO journal.

[16]  Jun Ma,et al.  Deletion analysis of GAL4 defines two transcriptional activating segments , 1987, Cell.

[17]  M. Vigneron,et al.  In vitro binding of cell‐specific and ubiquitous nuclear proteins to the octamer motif of the SV40 enhancer and related motifs present in other promoters and enhancers. , 1987, The EMBO journal.

[18]  B. Jarry,et al.  Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate‐resistance. , 1983, The EMBO journal.

[19]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[20]  W. Bender,et al.  The anterobithorax and bithorax mutations of the bithorax complex. , 1986, The EMBO journal.

[21]  L. Goldstein,et al.  Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells. , 1988, Nucleic acids research.

[22]  D. Davison,et al.  A detailed developmental and structural study of the transcriptional effects of insertion of the Copia transposon into the white locus of Drosophila melanogaster. , 1985, Genetics.

[23]  G. Fink,et al.  The SPT3 gene is required for normal transcription of Ty elements in S. cerevisiae , 1984, Cell.

[24]  V. Corces,et al.  On the molecular mechanism of gypsy‐induced mutations at the yellow locus of Drosophila melanogaster. , 1986, The EMBO journal.

[25]  W. Bender,et al.  Sequences of the gypsy transposon of Drosophila necessary for its effects on adjacent genes. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[26]  S. Parkhurst,et al.  forked, Gypsys, and suppressors in Drosophila , 1985, Cell.

[27]  R. Tjian,et al.  Characterization of drosophila transcription factors that activate the tandem promoters of the alcohol dehydrogenase gene , 1985, Cell.

[28]  L. L. Searles,et al.  Molecular characterization of the Drosophila vermilion locus and its suppressible alleles. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[29]  Finnegan Dj,et al.  Transposable elements in Drosophila melanogaster. , 1986 .

[30]  V. Williamson,et al.  Transposable elements in yeast. , 1983, International review of cytology.

[31]  J. Modolell,et al.  Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[32]  H. Schöler,et al.  A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes , 1987, Nature.

[33]  G. Rubin,et al.  Effects of transposable element insertions on RNA encoded by the white gene of Drosophila , 1984, Cell.

[34]  W. Gilbert,et al.  Sequencing end-labeled DNA with base-specific chemical cleavages. , 1980, Methods in enzymology.

[35]  V. Corces,et al.  Reversion of a gypsy-induced mutation at the yellow (y) locus of Drosophila melanogaster is associated with the insertion of a newly defined transposable element. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[36]  Stephen M. Mount,et al.  Partial revertants of the transposable element-associated suppressible allele white-apricot in Drosophila melanogaster: structures and responsiveness to genetic modifiers. , 1988, Genetics.

[37]  M. M. Garner,et al.  A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system , 1981, Nucleic Acids Res..

[38]  J. Modolell,et al.  Excess function Hairy-wing mutations caused by gypsy and copia insertions within structural genes of the achaete-scute locus of Drosophila , 1986, Cell.