A set of compatible tac promoter expression vectors.

A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacIq gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.

[1]  S. Altman,et al.  RNA-processing Nucleases , 1982 .

[2]  J. Brosius,et al.  Regulation of ribosomal RNA promoters with a synthetic lac operator. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[3]  T. Linn,et al.  Improved vector system for constructing transcriptional fusions that ensures independent translation of lacZ , 1990, Journal of bacteriology.

[4]  D. Denhardt,et al.  Vectors: A Survey of Molecular Cloning Vectors and Their Uses , 1987 .

[5]  D. Dykxhoorn,et al.  Synthesis of the β and β′ subunits of Escherichia coli RNA polymerase is autogenously regulated in vivo by both transcriptional and translational mechanisms , 1996 .

[6]  A. C. Chang,et al.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid , 1978, Journal of bacteriology.

[7]  T Platt,et al.  Transcription termination and the regulation of gene expression. , 1986, Annual review of biochemistry.

[8]  D. Bachvarov,et al.  Determination of plasmid copy number by the "boiling" method. , 1987, Analytical biochemistry.

[9]  B. Müller-Hill,et al.  The three operators of the lac operon cooperate in repression. , 1990, The EMBO journal.

[10]  H. Bujard,et al.  Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[11]  G. Churchward,et al.  The repA2 gene of the plasmid R100.1 encodes a repressor of plasmid replication. , 1983, Plasmid.

[12]  T. Linn,et al.  Autogenous regulation of the RNA polymerase beta subunit of Escherichia coli occurs at the translational level in vivo , 1989, Journal of bacteriology.

[13]  C. Kurland,et al.  Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction , 1995, Journal of bacteriology.

[14]  C. Yanisch-Perron,et al.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. , 1985, Gene.

[15]  G W Hatfield,et al.  Effects of promoter strengths and growth conditions on copy number of transcription-fusion vectors. , 1984, The Journal of biological chemistry.

[16]  V. Iyer,et al.  A portable DNA sequence carrying the cohesive site (cos) of bacteriophage lambda and the mob (mobilization) region of the broad-host-range plasmid RK2: a module for the construction of new cosmids. , 1984, Gene.

[17]  F. Bolivar,et al.  The plasmid, pBR322. , 1988, Biotechnology.

[18]  H. Noller,et al.  Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. , 1981, Journal of molecular biology.