Purine metabolism in neisseria meningitidis. 4. Interconversion of purine ribonucleotides.

Radioactive hypoxanthine and adenine were readily incorporated into the nucleic acids (RNA + DNA) of the prototrophic strain M1 of Neisseria meningitidis with labelling of the purine bases adenine and guanine. Contrary to this, adenine was incorporated into the nucleic acids with labelling of adenine only in the purine mutant 6-1c which requires adenine and guanine for growth. Guanine was in both strains incorporated into the nucleic acids with labelling of the purine base guanine only. Crude meningococcal extracts from the prototrophic strain M1 and strain 6-1b requiring histidine, proline, adenine and guanine for growth (his pro A G) were found to have activities corresponding to adenylosuccinate synthetase (EC 6.3.4.4), adenylosuccinate lyase (EC 4.3.2.2.), inosine 5'-phosphate dehydrogenase (EC 1.2.1.14)) and guanosine 5'-phosphate synthetase (EC 6.3.5.2). This last enzyme was active also with (NH4)2SO4 as amino donor. No activity corresponding to guanosine 5'-phosphate reductase (EC 1.6.6.8) could be demonstrated in either strain. The findings show that the mutant strains 6-1b and 6-1c are both blocked between aminoimidiazolcarboxamide ribonucleotide (AICA-R) and inosine 5'-phosphate (IMP). Transformation experiments with mutants requiring adenine and guanine for growth indicate that the negative property of these mutants is due to a lesion in one genetic locus or at least in loci that are very closely linked.

[1]  S. Jyssum.  Purine metabolism in Neisseria meningitidis. 1. Utilization of exogenous adenine. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[2]  S. Jyssum.  Utilization of thymine, thymidine and TMP by Neisseria meningitidis. 2. Lack of enzymes for specific incorporation of exogenous thymine, thymidine and TMP into DNA. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[3]  K. Jyssum,et al.  Utilization of thymine, thymidine and TMP by neisseria meningitidis. 1. Growth response and uptake of labelled material. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[4]  R. Miller,et al.  Xanthine phosphoribosyltransferase from Streptococcus faecalis. Properties and specificity. , 1974, Archives of biochemistry and biophysics.

[5]  S. Hartman,et al.  Preferential utilization of glutamine for amination of xanthosine 5'-phosphate to guanosine 5'-phosphate by purified enzymes from Escherichia coli. , 1974, Biochemical and biophysical research communications.

[6]  T. W. Wu,et al.  Properties of inosinic acid dehydrogenase from Bacillus subtilis. II. Kinetic properties. , 1973, Canadian journal of biochemistry.

[7]  R. Miller,et al.  Guanine phosphoribosyltransferase from Escherichia coli, specificity and properties. , 1972, Biochemistry.

[8]  R. Martin,et al.  Purine Phosphoribosyltransferases of Salmonella typhimurium , 1972, Journal of bacteriology.

[9]  S. Shumas,et al.  Genetic Separation of Hypoxanthine and Guanine-Xanthine Phosphoribosyltransferase Activities by Deletion Mutations in Salmonella typhimurium , 1972, Journal of bacteriology.

[10]  Debov Ss,et al.  Isolation and partial purification of purine nucleotide pyrophosphorylases from Brevibacterium ammoniagenes ATCC No. 6872 , 1971 .

[11]  E. Stadtman,et al.  The regulation of purine utilization in bacteria. II. Adenine phosphoribosyltransferase in isolated membrane preparations and its role in transport of adenine across the membrane. , 1971, The Journal of biological chemistry.

[12]  R. Miller,et al.  Guanine and xanthine phosphoribosyltransfer activities of Lactobacillus casei and Escherichia coli. Their relationship to hypoxanthine and adenine phosphoribosyltransfer activities. , 1970, The Journal of biological chemistry.

[13]  K. Rajagopalan,et al.  Purification and Properties of Inosinic Acid Dehydrogenase from Escherichia coli , 1969 .

[14]  G. Hitchings,et al.  Adenine phosphoribosyltransferase from monkey liver. Specificity and properties. , 1969, The Journal of biological chemistry.

[15]  T. Krenitsky,et al.  Human hypoxanthine phosphoribosyltransferase. II. Kinetics and chemical modification. , 1969, The Journal of biological chemistry.

[16]  G. Elion,et al.  Human hypoxanthine phosphoribosyltransferase. I. Purification, properties, and specificity. , 1969, The Journal of biological chemistry.

[17]  S. Kinoshita,et al.  Production of Nucleic Acid-Related Substances by Fermentative Processes: Part XXI. Biosynthetic Mechanisms Involved in 5′-Purine Ribonucleotide Production byBrevibacterium ammoniagenesPart XXII. Fermentative Production of 5′-Xanthylic Acid by a Guanine Auxotroph ofBrevibacterium ammoniagenes , 1969 .

[18]  I. Shiio,et al.  Regulation of purine ribonucleotide synthesis by end product inhibition. I. Effect of purine nucleotides on inosine-5'-phosphate dehydrogenase, xanthosine-5'-phosphate aminase and adenylosuccinate lyase of Bacillus subtilis. , 1968, Journal of biochemistry.

[19]  H. Nijkamp,et al.  Genetic and biochemical studies of the guanosine 5'-monophosphate pathway in Escherichia coli. , 1967, Biochimica et biophysica acta.

[20]  E. Stadtman,et al.  A possible role of purine nucleotide pyrophosphorylases in the regulation of purine uptake by Bacillus subtilis. , 1966, Journal of Biological Chemistry.

[21]  J. F. Henderson,et al.  Purification and properties of adenylate pyrophosphorylase from Ehrlich ascites tumor cells. , 1966, Journal of Biological Chemistry.

[22]  K. Jyssum.  Polarity of Chromosome Replication in Neisseria meningitidis , 1965, Journal of bacteriology.

[23]  S. Lie.  PRODUCTION OF AUXOTROPHIC MUTANTS OF NEISSERIA MENINGITIDIS BY NITROUS ACID. , 1965, Acta pathologica et microbiologica Scandinavica.

[24]  K. Jyssum,et al.  TRANSFORMATION OF AUXOTROPHS OF NEISSERIA MENINGITIDIS. , 1965, Acta pathologica et microbiologica Scandinavica.

[25]  K. Jyssum.  ISOLATION OF AUXOTROPHS FROM NEISSERIA MENINGITIDIS. , 1965, Acta pathologica et microbiologica Scandinavica.

[26]  G. P. Kalle,et al.  Alterations in purine nucleotide pyrophosphorylases and resistance to purine analogues. , 1961, Biochimica et biophysica acta.

[27]  D. Hutchison,et al.  Purine Ribonucleotide Pyrophosphorylases and Resistance to Purine Analogues in Streptococcus faecalis , 1961 .

[28]  J. Lederberg,et al.  Linkage of genetic units of Bacillus subtilis in DNA transformation. , 1961, Proceedings of the National Academy of Sciences of the United States of America.

[29]  C. Carter.  Reaction of 6-mercaptopurine with inosine- and guanosine-5'-phosphate pyrophosphorylase purified from E. coli. , 1959, Biochemical pharmacology.

[30]  U. Lagerkvist.  Biosynthesis of guanosine 5'-phosphate. II. Amination of xanthosine 5'-phosphate by purified enzyme from pigeon liver. , 1958, The Journal of biological chemistry.

[31]  B. Magasanik,et al.  Enzymes essential for the biosynthesis of nucleic acid guanine; xanthosine 5'-phosphate aminase of Aerobacter aerogenes. , 1957, The Journal of biological chemistry.

[32]  H. Krebs,et al.  Some reactions of adenosine and inosine phosphates in animal tissues. , 1953, Biochimica et biophysica acta.

[33]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[34]  B. D. Davis,et al.  MUTANTS OF ESCHERICHIA COLI REQUIRING METHIONINE OR VITAMIN B12 , 1950, Journal of bacteriology.

[35]  J. Adye,et al.  Further studies on genetically altered purine nucleotide pyrophosphorylases of Salmonella. , 1966, Biochimica et biophysica acta.

[36]  K. Jyssum,et al.  GENETIC FACTORS DETERMINING COMPETENCE IN TRANSFORMATION OF NEISSERIA MENINGITIDIS. 1. A PERMANENT LOSS OF COMPETENCE. , 1965, Acta pathologica et microbiologica Scandinavica.

[37]  K. Boevre.  STUDIES ON TRANSFORMATION IN MORAXELLA AND ORGANISMS ASSUMED TO BE RELATED TO MORAXELLA. 1. A METHOD FOR QUANTITATIVE TRANSFORMATION IN MORAXELLA AND NEISSERIA, WITH STREPLOMYCIN RESISTANCE AS THE GENETIC MARKER. , 1964, Acta pathologica et microbiologica Scandinavica.

[38]  R. Abrams,et al.  Biosynthesis of nucleic acid purines. III. Guanosine 5′-phosphate formation from xanthosine 5′-phosphate and l-glutamine , 1959 .

[39]  A. Bendich.  [106] Methods for characterization of nucleic acids by base composition , 1957 .