Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus.

A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.

[1]  S. Mccann.  ENDOCRINE PHYSIOLOGY. , 1931, British medical journal.

[2]  F. You,et al.  Sex steroid changes during temperature‐induced gonadal differentiation in Paralichthys olivaceus (Temminck & Schegel, 1846) , 2013 .

[3]  T. Swaminathan,et al.  Development, characterization, conservation and storage of fish cell lines: a review , 2011, Fish Physiology and Biochemistry.

[4]  F. Nan,et al.  Immunochemical and molecular characterization of a novel cell line derived from the brain of Trachinotus blochii (Teleostei, Perciformes): A fish cell line with oligodendrocyte progenitor cell and tanycyte characteristics. , 2010, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[5]  F. You,et al.  Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei) , 2010 .

[6]  A. Abdullah,et al.  Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation , 2010, In Vitro Cellular & Developmental Biology - Animal.

[7]  A. Levan,et al.  NOMENCLATURE FOR CENTROMERIC POSITION ON CHROMOSOMES , 2009 .

[8]  W. Zeng-xian.  The purification and culture of astrocytes from cerebral cortex of rats , 2007 .

[9]  G. Zupanc,et al.  Isolation, cultivation, and differentiation of neural stem cells from adult fish brain , 2006, Journal of Neuroscience Methods.

[10]  Z. Sha,et al.  Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation. , 2004, Diseases of aquatic organisms.

[11]  K. Kawai,et al.  Establishment and characterization of two new cell lines derived from flounder, Paralichthys olivaceus (Temminck & Schlegel). , 2003, Journal of fish diseases.

[12]  K. Fang,et al.  Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper irido- and nodaviruses. , 2003, Journal of fish diseases.

[13]  K. Fang,et al.  Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new nodavirus‐susceptible cell line from yellow grouper, Epinephelus awoara (Temminck & Schlegel), brain tissue , 2001 .

[14]  S. Tong,et al.  The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus , 1997 .

[15]  D E H Tee,et al.  Book Review: Culture of Animal Cells: A Manual of Basic Technique , 1984 .