Spermatic feasibility after 20 years of freezing and refreezing: case study

The aim of the present work was to study the spermatic feasibility after 20 years of freezing, thawing and refreezing. A young man affected by testicular cancer sought the sperm freezing service. After 20 years, the samples were thawed and frozen again. After two days, thawing and analysis of the material took place. Comparison of the findings showed activity reduction from the first thawing to the fresh semen 59.09%. For the second thawing it was 77.27%. Considering the morphology, first thawing showed a reduction of 44.64% in relation to fresh semen. In the second thawing, the reduction was of 75%. Spermatic motility decreased by 64.28% in relation to fresh semen and 92.85% at the level of second thawing. The patient had gone through in vitro fertilization in 2007, obtaining success in the first one attempt.

[1]  H. P. Godinho,et al.  Gene banking of the neotropical fish Leporinus obtusidens (Valenciennes, 1836): a protocol to freeze its sperm in the field. , 2008, Animal reproduction science.

[2]  C. Portwine,et al.  Effectiveness of sperm banking in adolescents and young adults with cancer , 2007, Cancer.

[3]  G. Clarke,et al.  Recovery of human sperm motility and ability to interact with the human zona pellucida after more than 28 years of storage in liquid nitrogen. , 2006, Fertility and sterility.

[4]  A. Ninhaus‐Silveira,et al.  Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869) , 2006 .

[5]  J. Feldschuh,et al.  Successful sperm storage for 28 years. , 2005, Fertility and sterility.

[6]  L. Weissbach,et al.  [Cryopreserved human sperm deposits: usability after decades of storage]. , 2005, Der Urologe. Ausg. A.

[7]  J. Logue,et al.  Live birth with sperm cryopreserved for 21 years prior to cancer treatment: case report. , 2004, Human reproduction.

[8]  J. Anger,et al.  Cryopreservation of sperm: indications, methods and results. , 2003, The Journal of urology.

[9]  W. Ryder,et al.  Semen cryopreservation, utilisation and reproductive outcome in men treated for Hodgkin's disease , 2002, British Journal of Cancer.

[10]  N. Mcclure,et al.  Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation. , 2001, Human reproduction.

[11]  A. S. Parkes,et al.  Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures , 1949, Nature.

[12]  P. F. Taitson,et al.  Incidence of male infertility by occupational factors In the State of Minas Gerais , 2008, JBRA Assisted Reproduction.

[13]  A. Ninhaus‐Silveira,et al.  Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss , 2006 .