MICROWAVES SYNTHESIS OF SOLID SUPPORTS FOR THE SYNTHESIS OF 3′-AMINOALKYL OLIGODEOXYNUCLEOTIDES

Phthalimido-alkyl alcohol solid supports were rapidly prepared from solid supported phthalic anhydride and amino alcohol condensation induced by microwaves. These supports were used to synthesize 3′-aminoalkyl oligodeoxynucleotides allowing a two step deprotection necessary to avoid aminolink alkylation.

[1]  M. Leuck,et al.  A novel solid support for the synthesis of 3′-aminoalkylated oligonucleotides , 2004 .

[2]  J. Rademann,et al.  Trimellitic anhydride linker (TAL)—highly orthogonal conversions of primary amines employed in the parallel synthesis of labeled carbohydrate derivatives ☆ ☆☆ , 2003 .

[3]  J. Imbach,et al.  STEP-BY-STEP CONTROL BY MALDI-TOF MS OF AN OLIGONUCLEOTIDE SYNTHESIS ON STANDARD CPG SOLID-SUPPORT , 2001, Nucleosides, Nucleotides & Nucleic Acids.

[4]  X. Qian,et al.  IMIDATION OF CYCLIC CARBOXYLIC ANHYDRIDES UNDER MICROWAVE IRRADIATION , 2001 .

[5]  A. Petit,et al.  Re-examination of Microwave-Induced Synthesis of Phthalimides , 2000 .

[6]  M. Lyttle,et al.  Versatile linker chemistry for synthesis of 3'-modified DNA. , 1997, Bioconjugate chemistry.

[7]  M. Khan.  Suggested Improvement in the Ing-Manske Procedure and Gabriel Synthesis of Primary Amines: Kinetic Study on Alkaline Hydrolysis of N-Phthaloylglycine and Acid Hydrolysis of N-(o-Carboxybenzoyl)glycine in Aqueous Organic Solvents. , 1996, The Journal of organic chemistry.

[8]  K. Gupta,et al.  Universal polymer support for the synthesis of 3′-end modified oligonucleotides , 1996 .

[9]  P. S. Nelson,et al.  Oligonucleotide labeling methods. 3. Direct labeling of oligonucleotides employing a novel, non-nucleosidic, 2-aminobutyl-1,3-propanediol backbone. , 1992, Nucleic acids research.

[10]  C. R. Petrie,et al.  An improved CPG support for the synthesis of 3'-amine-tailed oligonucleotides. , 1992, Bioconjugate chemistry.

[11]  N. Thuong,et al.  New Solid-Phase for Automated Synthesis of Oligonucleotides Containing an Amino-Alkyl Linker at Their 3′-End. , 1990 .

[12]  U. Asseline,et al.  New solid-phase for automated synthesis of oligonucleotides containing an amino-alkyl linker at their 3′-end , 1990 .