Expression and function of aquaporin-1 in hyperoxia-exposed alveolar epithelial type II cells

The aim of the present study was to investigate water transport dysfunction in alveolar epithelial type II cells (AECII), which were exposed to hyperoxia, and to investigate the mechanism of pulmonary edema resulting from hyperoxic lung injury. The lung cells of newborn rats were isolated for primary cell culture and divided into control and experimental groups. The control and experimental group cells were placed into a normoxic incubator (oxygen volume fraction, 0.21) or hyperoxic incubator (oxygen volume fraction, 0.9), respectively. Twenty-four, 48 and 72 h after cell attachment, the gene transcription and protein expression levels of aquaporin-1 (AQP1) were detected via quantitative polymerase chain reaction and western blot analysis. Flow cytometry was conducted to detect the volume of the cells in the experimental and control groups. In the present study, it was identified that AQP1 expression and cell volume were greater in the experimental group when compared with the control group. Thus, hyperoxia may disturb the gene expression regulation of AQP1 in AECII, resulting in water transport dysfunction. This may be one of the mechanisms underlying pulmonary edema caused by hyperoxic lung injury.

[1]  T. Chou,et al.  Baicalein, an active component of Scutellaria baicalensis, protects against lipopolysaccharide-induced acute lung injury in rats. , 2014, Journal of ethnopharmacology.

[2]  T. Billiar,et al.  Hydrogen inhalation reduced epithelial apoptosis in ventilator-induced lung injury via a mechanism involving nuclear factor-kappa B activation. , 2011, Biochemical and biophysical research communications.

[3]  Bo Zhang,et al.  Tanshinone IIA ameliorates seawater exposure-induced lung injury by inhibiting aquaporins (AQP) 1 and AQP5 expression in lung , 2011, Respiratory Physiology & Neurobiology.

[4]  Xue Xin-dong.  Ultrastructure Changes of Alveolar Epithelia Type II Cells in Newborn Rats with Chronic Lung Disease Induced by Hyperoxia , 2011 .

[5]  Heguang Huang,et al.  Acute lung injury and change in expression of aquaporins 1 and 5 in a rat model of acute pancreatitis. , 2010, Hepato-gastroenterology.

[6]  H. Shibuya,et al.  CHIP‐dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response , 2010, The EMBO journal.

[7]  C. Bai,et al.  Expression of aquaporin 5 increases proliferation and metastasis potential of lung cancer , 2010, The Journal of pathology.

[8]  H. Sontheimer,et al.  MAPK induces AQP1 expression in astrocytes following injury , 2010, Glia.

[9]  C. Delacourt,et al.  Pulmonary effects of keratinocyte growth factor in newborn rats exposed to hyperoxia. , 2009, American journal of physiology. Lung cellular and molecular physiology.

[10]  B. Zang,et al.  Dobutamine Enhances Alveolar Fluid Clearance in a Rat Model of Acute Lung Injury , 2009, Lung.

[11]  Carissa M Krane,et al.  Altered regulation of aquaporin gene expression in allergen and IL-13-induced mouse models of asthma. , 2009, Cytokine.

[12]  Keishi Kubo,et al.  Acute lung injury review. , 2009, Internal medicine.

[13]  Erran L. Li,et al.  Accumulation of aquaporin-1 during hemolysininduced necrotic cell death , 2008, Cellular & Molecular Biology Letters.

[14]  A. Verkman.  Role of aquaporins in lung liquid physiology , 2007, Respiratory Physiology & Neurobiology.

[15]  J. Sznajder,et al.  Regulation of Na,K-ATPase during acute lung injury , 2007, Journal of bioenergetics and biomembranes.

[16]  R. Wurtman,et al.  Oral L‐glutamine increases GABA levels in striatal tissue and extracellular fluid , 2007, The FASEB Journal.

[17]  G. Miserocchi,et al.  Biochemical and morphological changes in endothelial cells in response to hypoxic interstitial edema , 2006, Respiratory research.

[18]  S. Sasaki,et al.  Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells. , 2005, Biochemical and biophysical research communications.

[19]  A. Aperia,et al.  Water Channels (Aquaporins) and Their Role for Postnatal Adaptation , 2005, Pediatric Research.

[20]  Peter Agre,et al.  Aquaporin water channels: molecular mechanisms for human diseases1 , 2003, FEBS letters.

[21]  N. Modi.  Clinical implications of postnatal alterations in body water distribution. , 2003, Seminars in neonatology : SN.

[22]  R. Bucala,et al.  Role for macrophage migration inhibitory factor in acute respiratory distress syndrome , 2003, The Journal of pathology.

[23]  A. Verkman,et al.  Lung edema clearance: 20 years of progress: invited review: role of aquaporin water channels in fluid transport in lung and airways. , 2002, Journal of applied physiology.

[24]  P. Agre,et al.  Decreased pulmonary vascular permeability in aquaporin-1-null humans , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[25]  R. Boucher,et al.  Expression and localization of epithelial aquaporins in the adult human lung. , 2001, American journal of respiratory cell and molecular biology.

[26]  A. Verkman,et al.  Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice , 2000, The Journal of physiology.

[27]  Yuanlin Song,et al.  Role of Aquaporin-4 in Airspace-to-Capillary Water Permeability in Intact Mouse Lung Measured by a Novel Gravimetric Method , 2000, The Journal of general physiology.

[28]  A. Verkman,et al.  Lung fluid transport in aquaporin-5 knockout mice. , 2000, The Journal of clinical investigation.

[29]  Carissa M Krane,et al.  Decreased expression of aquaporin (AQP)1 and AQP5 in mouse lung after acute viral infection. , 2000, American journal of respiratory cell and molecular biology.

[30]  A. Verkman,et al.  Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice. , 1999, The Journal of clinical investigation.

[31]  M. K. Ruddy,et al.  Modulation of aquaporin 4 and the amiloride-inhibitable sodium channel in perinatal rat lung epithelial cells. , 1998, American journal of physiology. Lung cellular and molecular physiology.

[32]  P. Agre,et al.  Genomic organization and developmental expression of aquaporin-5 in lung. , 1997, Chest.

[33]  C. Jordan,et al.  High-resolution cell cycle analysis of defined phenotypic subsets within primitive human hematopoietic cell populations. , 1996, Experimental hematology.

[34]  P. Agre,et al.  Pathophysiology of the aquaporin water channels. , 1996, Annual review of physiology.

[35]  M. Williams,et al.  An improved method for isolating type II cells in high yield and purity. , 2015, The American review of respiratory disease.