Dielectrophoretic properties of engineered protein patterned colloidal particles.

This work determines the dielectrophoretic response of surface modified polystyrene and silica colloidal particles by experimentally measuring their Clausius-Mossotti factors. Commercial charged particles, fabricated ones coated with fibronectin, and Janus particles that have been grafted with fibronectin on one side only were investigated. We show that the dielectrophoretic response of such particles can be controlled by the modification of the chemistry or the anisotropy of their surface. Moreover, by modelling the polarizabilities of those particles, the dielectric parameters of the particles and the grafted layer of protein can be measured.

[1]  A. Warshel,et al.  What are the dielectric “constants” of proteins and how to validate electrostatic models? , 2001, Proteins.

[2]  Quantitative measurements of absolute dielectrophoretic forces using optical tweezers. , 2010, Optics letters.

[3]  Ronald Pethig,et al.  Dielectrophoretic studies of the activation of human T lymphocytes using a newly developed cell profiling system , 2002, Electrophoresis.

[4]  H Morgan,et al.  3D focusing of nanoparticles in microfluidic channels. , 2003, IEE proceedings. Nanobiotechnology.

[5]  Otto L Muskens,et al.  Interactions of human endothelial cells with gold nanoparticles of different morphologies. , 2012, Small.

[6]  H. P. Schwan,et al.  Surface conductance and other properties of latex particles measured by electrorotation , 1987 .

[7]  Luke P. Lee,et al.  Bioinspired nanocorals with decoupled cellular targeting and sensing functionality. , 2010, Small.

[8]  Emmanuel Picard,et al.  Determination of Clausius–Mossotti factors and surface capacitances for colloidal particles , 2011 .

[9]  Á. Delgado,et al.  Surface conductivity of colloidal particles: experimental assessment of its contributions. , 2007, Journal of colloid and interface science.

[10]  Joel Voldman,et al.  Attenuation of extrinsic signaling reveals the importance of matrix remodeling on maintenance of embryonic stem cell self-renewal , 2012, Proceedings of the National Academy of Sciences.

[11]  F. L. Yap,et al.  Protein and cell micropatterning and its integration with micro/nanoparticles assembly. , 2007, Biosensors & bioelectronics.

[12]  R. Pethig.  Review article-dielectrophoresis: status of the theory, technology, and applications. , 2010, Biomicrofluidics.

[13]  R. Pethig,et al.  Dielectrophoretic detection of membrane morphology changes in Jurkat T-cells undergoing etoposide-induced apoptosis. , 2007, IET nanobiotechnology.

[14]  H. Morgan,et al.  The electrokinetic properties of latex particles: comparison of electrophoresis and dielectrophoresis. , 2005, Journal of colloid and interface science.

[15]  Tomoyuki Yasukawa,et al.  Negative dielectrophoretic patterning with colloidal particles and encapsulation into a hydrogel. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[16]  Arnan Mitchell,et al.  Novel tuneable optical elements based on nanoparticle suspensions in microfluidics , 2010, Electrophoresis.

[17]  T. Honegger,et al.  Selective grafting of proteins on Janus particles: Adsorption and covalent coupling strategies , 2011 .

[18]  R. J. Hunter,et al.  Measurement and Interpretation of Electrokinetic Phenomena (IUPAC Technical Report) , 2005 .

[19]  H. Morgan,et al.  Electrohydrodynamics and dielectrophoresis in microsystems: scaling laws , 2003 .

[20]  S. D. Collins,et al.  Frequency dependence of gold nanoparticle superassembly by dielectrophoresis. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[21]  T. Honegger,et al.  Design and realization of a microfluidic system for dielectrophoretic colloidal handling , 2009 .

[22]  Hsueh-Chia Chang,et al.  Induced dipoles and dielectrophoresis of nanocolloids in electrolytes. , 2007, Physical review. E, Statistical, nonlinear, and soft matter physics.

[23]  H. Ou-yang,et al.  Direct measurements of the frequency-dependent dielectrophoresis force. , 2009, Biomicrofluidics.

[24]  Minnamari Vippola,et al.  Proteomic characterization of engineered nanomaterial-protein interactions in relation to surface reactivity. , 2011, ACS nano.

[25]  Hsien-Hung Wei,et al.  Dynamic double layer effects on ac-induced dipoles of dielectric nanocolloids. , 2010, Biomicrofluidics.

[26]  W. Boireau,et al.  Strategy of macromolecular grafting onto a gold substrate dedicated to protein-protein interaction measurements. , 2006, Biosensors & bioelectronics.

[27]  Chester T. O'Konski,et al.  ELECTRIC PROPERTIES OF MACROMOLECULES. V. THEORY OF IONIC POLARIZATION IN POLYELECTROLYTES , 1960 .

[28]  Yingxi Zhu,et al.  Dielectrophoresis of Janus particles under high frequency ac-electric fields , 2010 .

[29]  Cheng-Hsien Liu,et al.  Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap. , 2006, Lab on a chip.

[30]  M. Vuento,et al.  Solution structure of human plasma fibronectin using small-angle X-ray and neutron scattering at physiological pH and ionic strength. , 1987, Archives of biochemistry and biophysics.

[32]  J. Pitera,et al.  Dielectric properties of proteins from simulation: the effects of solvent, ligands, pH, and temperature. , 2001, Biophysical journal.

[33]  Hywel Morgan,et al.  Dielectrophoresis of Submicrometer Latex Spheres. 1. Experimental Results , 1999 .

[34]  Hsueh-Chia Chang,et al.  Designing a sensitive and quantifiable nanocolloid assay with dielectrophoretic crossover frequencies. , 2010, Biomicrofluidics.

[35]  Hughes,et al.  The Dielectrophoretic Behavior of Submicron Latex Spheres: Influence of Surface Conductance. , 1999, Journal of colloid and interface science.