Cytological Analysis of Macro‐ and Microsporogenesis of a Diploid Alfalfa Clone Producing Male and Female 2n Gametes

Gametes with the somatic chromosome number (2n gametes) are important in germplasm transfer and in the evolution of polyploidy. In this work a stain-clearing methodology war applied to the cytological study of both macrosprorogenesis and microsprorogenesis in the diploid (2n=2x=16) clone H33 of alfalfa (Medicago sativa L.), which produces both male and female 2n gametes