Tethered mediator biosensor. Mediated electron transfer between redox enzyme and electrode via ferrocene anchored to electrode surface with long poly(oxyethylene) chain

In order to build an electronic interface between a redox enzyme and an electrode, we have designed a new mediator system, where the mediator molecule is immobilized onto an electrode surface via a long flexible chain-“tethered” mediator. As a prototype tethered mediator system, a ferrocene derivative with a long poly(oxyethylene) chain was immobilized onto an SnO2 electrode and electron mediation function to horseradish peroxidase (HRP) was examined. In the cases of both soluble and co-immobilized HRP, we observed a catalytic current for hydrogen peroxide reduction. The kinetic analysis exhibited that the tethered ferrocene effectively mediates electron between the enzyme and the electrode.

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