Integrated fluorescent light source for optofluidic applications

This letter describes a simple fluidic light source for use “on-chip” in integrated microsystems. It demonstrates the feasibility of light sources based on liquid-core, liquid-cladding (L2) microchannel waveguides, with liquid cores containing fluorescent dyes. These fluorescent light sources, using both miscible and two-phase systems, are tunable in terms of the beam size, intensity and spectral content. The observed output intensity from fluorescent L2 light sources is comparable to standard fiber optic spectrophotometer light sources. Integration of fluorescent light sources during device fabrication removes both the need for insertion and alignment of conventional, optical-fiber light sources and the constraints on channel size imposed by fiber optics, albeit at the cost of establishing a microfluidic infrastructure.

[1]  大房 健 基礎講座 電気泳動(Electrophoresis) , 2005 .

[2]  R. J. Dijkstra,et al.  Liquid-core waveguide technology for coupling column liquid chromatography and Raman spectroscopy. , 2001, Journal of chromatography. A.

[3]  Howard A. Stone,et al.  ENGINEERING FLOWS IN SMALL DEVICES , 2004 .

[4]  A. Manz,et al.  Micro total analysis systems. Recent developments. , 2004, Analytical chemistry.

[5]  Th. Förster,et al.  Handbook of Fluorescence Spectra of Aromatic Molecules , 1973 .

[6]  E. Verpoorte Chip vision-optics for microchips. , 2003, Lab on a chip.

[7]  Z. Fang,et al.  A miniaturized liquid core waveguide-capillary electrophoresis system with flow injection sample introduction and fluorometric detection using light-emitting diodes. , 2001, Analytical chemistry.

[8]  J. Westberg,et al.  A liquid core waveguide fluorescence detector for multicapillary electrophoresis applied to DNA sequencing in a 91‐capillary array , 2000, Electrophoresis.

[9]  H. Franke,et al.  A cylindrical liquid-core waveguide , 1996 .

[10]  G. Whitesides,et al.  Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. , 2002, Accounts of chemical research.

[11]  Successes and challenges of lab-on-a-chip. , 2002, Lab on a chip.

[12]  Stephane Mouradian,et al.  Lab-on-a-chip: applications in proteomics. , 2002, Current opinion in chemical biology.

[13]  George M. Whitesides,et al.  Fabrication of Liquid‐Core Waveguides by Soft Lithography , 1999 .

[14]  G. Whitesides,et al.  Dynamic control of liquid-core/liquid-cladding optical waveguides , 2004, (CLEO). Conference on Lasers and Electro-Optics, 2005..

[15]  J. Olivares,et al.  Liquid core waveguide for full imaging of electrophoretic separations. , 2002, Analytical chemistry.

[16]  J. Pawliszyn,et al.  Capillary isoelectric focusing of proteins with liquid core waveguide laser-induced fluorescence whole column imaging detection. , 2003, Analytical chemistry.