First Report of Blastocystis Infection in Pigs from Large Farms in Xinjiang, China

Blastocystis is a common intestinal protozoan in humans and various animals worldwide. A few studies have reported the genetic characterization of Blastocystis in pigs in China, but no epidemiological data are available from the Xinjiang Uygur Autonomous Region. In this study, 801 fecal samples were collected from seven scale pig farms in Xinjiang and tested by polymerase chain reaction and sequence analysis of the partial SSU rRNA gene. The average infection rate of Blastocystis was 21.7% (174/801), with 7.1% in preweaning piglets, 10.0% in postweaning piglets, 31.8% in fattening pigs, and 41.9% in sows (χ2 = 104.89; P < 0.01). Blastocystis subtypes ST1 (7/174), ST3 (2/174), and ST5 (165/174) were identified, with subtype ST5 being predominant in each of the pig farms and in each of the age groups. ST3 and ST5 were identified in preweaning piglets, and ST1, ST3, and ST5 were identified in postweaning piglets. In contrast, only the subtype ST5 was observed in fattening pigs and sows. Genetic polymorphisms were observed at the intrasubtype level, including two variations of ST1 (ST1A, ST1B), and seven of ST5 (ST5A to ST5G), by sequence alignment analysis and phylogenetic analysis. More studies are needed to elucidate the transmission and public health significance of Blastocystis in pigs in various areas.

[1]  B. Jung,et al.  Molecular Detection and Subtyping of Blastocystis in Korean Pigs , 2019, The Korean journal of parasitology.

[2]  J. Ramírez,et al.  A summary of Blastocystis subtypes in North and South America , 2019, Parasites & Vectors.

[3]  Z. Zhong,et al.  Epidemiology of Blastocystis sp. infection in China: a systematic review , 2019, Parasite.

[4]  Q. Lin,et al.  First genotyping of Blastocystis in yaks from Qinghai Province, northwestern China , 2019, Parasites & Vectors.

[5]  Fatih Çakır,et al.  Determination the Subtypes of Blastocystis sp. and Evaluate the Effect of These Subtypes on Pathogenicity , 2019, Acta Parasitologica.

[6]  T. Changbunjong,et al.  Blastocystis subtype 5: Predominant subtype on pig farms, Thailand. , 2018, Parasitology international.

[7]  T. Changbunjong,et al.  Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand. , 2018, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[8]  A. Pedrosa,et al.  Occurrence of Blastocystis spp. in domestic animals in Triângulo Mineiro area of Brazil. , 2018, Revista da Sociedade Brasileira de Medicina Tropical.

[9]  Jianguang Wang,et al.  Subtype distribution and genetic characterizations of Blastocystis in pigs, cattle, sheep and goats in northeastern China's Heilongjiang Province. , 2018, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[10]  C. Sreekumar,et al.  Prevalence and staining characteristics of Blastocystis isolates from food animals in Tamil Nadu. , 2018, Veterinary parasitology, regional studies and reports.

[11]  Longxian Zhang,et al.  Zoonotic and host-adapted genotypes of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in dairy cattle in Hebei and Tianjin, China. , 2017, Veterinary parasitology.

[12]  Xian-Cheng Fan,et al.  Molecular characterization of Blastocystis from pigs in Shaanxi province of China. , 2017, Acta tropica.

[13]  I. Rozi,et al.  Molecular survey of Blastocystis sp. from humans and associated animals in an Indonesian community with poor hygiene. , 2016, Parasitology international.

[14]  R. Traub,et al.  Characterization of the Blastocystis‐specific faecal IgA immune response in pigs , 2014, Parasite immunology.

[15]  T. Inpankaew,et al.  Molecular epidemiology of Blastocystis in pigs and their in-contact humans in Southeast Queensland, Australia, and Cambodia. , 2014, Veterinary parasitology.

[16]  R. Fayer,et al.  Blastocystis tropism in the pig intestine , 2014, Parasitology Research.

[17]  C. Texier,et al.  Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis , 2013, Therapeutic advances in infectious disease.

[18]  C. Clark,et al.  Genetic diversity of blastocystis in livestock and zoo animals. , 2013, Protist.

[19]  R. Fayer,et al.  Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals , 2011, Parasitology Research.

[20]  C. Clark,et al.  Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. , 2009, International journal for parasitology.

[21]  R. Borrás,et al.  High prevalence of Blastocystis sp. in pigs reared under intensive growing systems: frequency of ribotypes and associated risk factors. , 2008, Veterinary parasitology.

[22]  K. Mølbak,et al.  Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. , 2007, Diagnostic microbiology and infectious disease.

[23]  Zhiping Hou,et al.  Blastocystis sp. subtype 5: a possibly zoonotic genotype , 2007, Parasitology Research.

[24]  W. Rivera,et al.  Molecular characterization of Blastocystis isolates in the Philippines by riboprinting , 2005, Parasitology Research.

[25]  Zhiliang Wu,et al.  PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds. , 2004, Microbiology.

[26]  U. Thathaisong,et al.  Blastocystis Isolates from a Pig and a Horse Are Closely Related to Blastocystis hominis , 2003, Journal of Clinical Microbiology.

[27]  H. Yoshikawa,et al.  A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan. , 2002, Veterinary parasitology.

[28]  Peter J. Schaap,et al.  Molecular characterization of the , 1997 .

[29]  J. Quílez,et al.  Detection of Blastocystis sp. in pigs in Aragon (Spain). , 1995, Veterinary parasitology.

[30]  M. Pakandl.  Occurrence of Blastocystis sp. in pigs. , 1991, Folia parasitologica.

[31]  H. Anger,et al.  Blastocystis sp. infections in pigs , 1979 .