As the use of data independent acquisition (DIA) grows in proteomics research, the need for improved data processing workflows increases. The most common DIA data processing workflow is to use spectral ion libraries to drive targeted extraction of peptide / fragment peak areas from the data, using the m/z and retention time information contained in the library. Increasing the size and quality of the ion library has been shown to increase the number of proteins reliably detected and quantified from a dataset 1 . Retention time (RT) correlation between ion library and the dataset is another key factor that determines quality of data extraction. Currently, the user either doses in a standard peptide mix or manually selects endogenous peptides to be used as retention time markers between the data and the ion library.