Effect of lactic acid and acetic acid on the quality of local meat

Effect of lactic acid and acetic acid spray on the quality and shelf-life extension of fresh beef stored at 4 o C was investigated. Lactic and acetic acid were sprayed over the meat at various concentrations of 0 (control), 2% lactic acid (L.A.), 4% L.A, 2% acetic acid (A.A.) and 4% A.A. then stored at 4 o C for 9 days. Samples were examined in the fresh state (0 day), prior to spay. At specified time (30 min,1, 3, 5, 7 and 9 days) ,samples were analyzed for microbial counts, sensorial properties, pH and total volatile basic nitrogen(TVB-N). Bacterial groups count to indicate effectiveness of treatments included aerobic plate counts (APCs), Psychrophilic counts (PTCs) and total coliform counts (TCCs).Since the beginning of the experiment, the inhibitory effect of lactic acid and acetic acid has been observed. Lactic and acetic acid reduced the microbial counts immediately after the treatment and retarded microbial growth during storage . At first day of display, APCs were reduced by 1.5 to 3 log cycles, log psychrophilic counts were consistently reduced and log coliform counts were reduced to undetectable levels in acid treated groups. The inhibitory effect on bacterial growth was pronounced when the concentration of lactic acetic and lactic acids increased. Acetic and lactic acid treatments improved the microbiological and physicochemical qualities of meat and consequently prolongs their shelf life. Shelf-life of acid treated samples from microbial quality standpoints was 7 -9 days according to treatment used against 3 days in untreated samples. Four percent lactic acid treatments appeared to be more effective in delaying the microbial growth however; meat sample could not keep their color and odor. Comparing the effects of the two acids treatments, lactic acid was found to be more effective in both concentrations used. Lactic acid sprayed samples, particularly with 2%, showed the greater acceptability than did those sprayed with acetic acid throughout the storage days. From microbial and visual quality standpoints, meat sample sprayed with 2% seemed to be more acceptable regarding meat color and bacterial numbers with proper and could provide considered significant if less than 0.05 and highly significant if less than 0.01. These tests were analyzed using the Statistical for Scientists (SPSS)

[1]  J. Ubua,et al.  Effect of Refrigeration and Frozen Storage on the Shelf-life of Beef Purchased from Local Markets and Abattoir in Calabar Metropolis-Nigeria , 2007 .

[2]  G. Kandeepan,et al.  Effect of Low Temperature Preservation on Quality and Shelf Life of Buffalo Meat , 2007 .

[3]  D. Harris Application of antimicrobial treatments in a commercial simulation to reduce E. coli O157:H7 and Salmonella spp. in beef trim and in ground beef , 2006 .

[4]  Z. Min,et al.  Effect of lactic acid pretreatment on the quality of fresh pork packed in modified atmosphere , 2006 .

[5]  K. Grunert,et al.  Consumer perception of the quality of beef resulting from various fattening regimes , 2005 .

[6]  P. Pipek,et al.  Microbial decontamination of beef carcasses by combination of steaming and lactic acid spray , 2005 .

[7]  M. Kontominas,et al.  Microbiological, biochemical and sensory assessment of mussels (Mytilus galloprovincialis) stored under modified atmosphere packaging , 2005, Journal of applied microbiology.

[8]  E. Hilario,et al.  Improved resolution on the phylogenetic relationships among Pseudomonas by the combined analysis of atpD, carA, recA and 16S rDNA , 2004, Antonie van Leeuwenhoek.

[9]  C. Cutter,et al.  Comparison of electrolyzed oxidizing water with other antimicrobial interventions to reduce pathogens on fresh pork. , 2004, Meat science.

[10]  C. Gill,et al.  Effects of peroxyacetic acid, acidified sodium chlorite or lactic acid solutions on the microflora of chilled beef carcasses. , 2004, International journal of food microbiology.

[11]  Taner Baysal,et al.  Decontamination Techniques of Pathogen Bacteria in Meat and Poultry , 2004, Critical reviews in microbiology.

[12]  C. Gill,et al.  Microbiological effects of carcass decontaminating treatments at four beef packing plants. , 2003, Meat science.

[13]  Joong-Wan Kim,et al.  Comparison of indicators of microbial quality of meat during aerobic cold storage. , 2003, Journal of food protection.

[14]  Total and Intramuscular Bacterial Populations of Carcasses and Cuts , 2003 .

[15]  R. Huffman,et al.  Current and future technologies for the decontamination of carcasses and fresh meat. , 2002, Meat science.

[16]  Lone Gram,et al.  Food spoilage--interactions between food spoilage bacteria. , 2002, International journal of food microbiology.

[17]  J. Apple,et al.  The effects of acetic acid, gluconic acid and trisodium citrate treatment of beef trimmings on microbial, color and odor characteristics of ground beef through simulated retail display. , 2002, Meat science.

[18]  J. Sofos,et al.  Exposure to non‐acid fresh meat decontamination washing fluids sensitizes Escherichia coli O157:H7 to organic acids , 2002, Letters in applied microbiology.

[19]  V. Jayesh Effect of chilling and freezing on physico-chemical quality of mutton , 2002 .

[20]  J. Savell,et al.  Lactic acid and trisodium phosphate treatment of lamb breast to reduce bacterial contamination. , 2001, Journal of food protection.

[21]  A Castillo,et al.  In-Plant evaluation of a lactic acid treatment for reduction of bacteria on chilled beef carcasses.. , 2001, Journal of food protection.

[22]  A. I. Negueruela,et al.  Beef colour evolution as a function of ultimate pH. , 2001, Meat science.

[23]  K.‐W. Lin,et al.  Effectiveness of Dipping with Phosphate, Lactate and Acetic Acid Solutions on the Quality and Shelf‐life of Pork Loin Chop , 2001 .

[24]  J. Lahr Beef Carcass Microbial Contamination – Post Slaughter Numbers of Bacteria, Sources of Contamination and Variability of Data , 2001 .

[25]  M. Hunt Guidelines for Meat Color Evaluation , 2001 .

[26]  Torben Jensen Decontamination of Pig Carcasses with Hot Water , 2001 .

[27]  L. Leistner,et al.  Basic aspects of food preservation by hurdle technology. , 2000, International journal of food microbiology.

[28]  E. Berry,et al.  Effects of Acid Adaptation of Escherichia coli O157:H7 on Efficacy of Acetic Acid Spray Washes To Decontaminate Beef Carcass Tissue , 2000, Applied and Environmental Microbiology.

[29]  J. Sofos,et al.  Nonacid meat decontamination technologies: model studies and commercial applications. , 1998, International journal of food microbiology.

[30]  Heikki Kallio,et al.  The Effect of Lactic Acid, Nisin Whey Permeate, Sodium Chloride and Related Combinations on Aerobic Plate Count and the Sensory Characteristics of Rainbow Trout☆ , 1998 .

[31]  J. M. Jay Intrinsic and Extrinsic Parameters of Foods That Affect Microbial Growth , 1998 .

[32]  Vasiliki R. Kyrana,et al.  Assessment of shelf-life of maricultured gilthead sea bream (Sparus aurata) stored in ice , 1997 .

[33]  N. M. Bolder,et al.  Decontamination of meat and poultry carcasses , 1997 .

[34]  G C Smith,et al.  Effect of water temperature, pressure and chemical solution on removal of fecal material and bacteria from lamb adipose tissue by spray-washing. , 1997, Meat science.

[35]  E. Borch,et al.  Bacterial spoilage of meat and cured meat products. , 1996, International journal of food microbiology.

[36]  D. D. Bradford,et al.  Muscle Microstructure and Sensory Attributes of Organic Acid-Treated Beef Strip Loins , 1996 .

[37]  K. Scheller,et al.  Color coordinates for assessment of dietary vitamin E effects on beef color stability. , 1996, Journal of animal science.

[38]  J. Savell,et al.  Comparison of Methods for Decontamination from Beef Carcass Surfaces. , 1995, Journal of food protection.

[39]  D. Mossel,et al.  An in-vitro meat model for the immediate bactericidal effect of lactic acid decontamination on meat surfaces. , 1994, The Journal of applied bacteriology.

[40]  K. Young,et al.  Acetic, lactic and citric acids and pH inhibition of Listeria monocytogenes Scott A and the effect on intracellular pH. , 1993, The Journal of applied bacteriology.

[41]  B M Mackey,et al.  The relationship between the phenotypic properties of bacteria from chill-stored meat and spoilage processes. , 1992, Society for Applied Bacteriology symposium series.

[42]  G. Siragusa,et al.  Inhibition of Listeria monocytogenes on beef tissue by application of organic acids immobilized in a calcium alginate gel. , 1992 .

[43]  M. E. Anderson,et al.  Microbiological Decontamination of Food Animal Carcasses by Washing and Sanitizing Systems: A Review. , 1992, Journal of food protection.

[44]  J. B. Morgan,et al.  Microbiological effects of acid decontamination of pork carcasses at various locations in processing. , 1992, Meat science.

[45]  K. Cudjoe The effect of lactic acid sprays on the keeping qualities of meat during storage. , 1988, International journal of food microbiology.

[46]  H. R. Cross,et al.  FACTORS ASSOCIATED WITH FRESH MEAT COLOR: A REVIEW , 1984 .

[47]  C. Vanderzant,et al.  Microbiological and Chemical Changes During Storage of Swordfish ( Xiphias gladius ) Steaks in Retail Packages Containing CO2-Enriched Atmospheres. , 1982, Journal of Food Protection.

[48]  E. D. Strange,et al.  SIMPLIFIED METHODOLOGY FOR MEASURING MEAT COLOR , 1974 .

[49]  C. Sheu,et al.  Function of Lipophilic Acids as Antimicrobial Food Additives , 1973, Nature.

[50]  L. E. Jeremiah,et al.  BEEF COLOR AS RELATED TO CONSUMER ACCEPTANCE AND PALATABILITY , 1972 .

[51]  O. Wilde,et al.  “Food Additives” , 1951 .

[52]  B. M. Watts Oxidative rancidity and discoloration in meat. , 1954, Advances in food research.