Self-assembly of brome mosaic virus capsids: insights from shorter time-scale experiments.

An amended kinetic model for the self-assembly of empty capsids of brome mosaic virus is proposed. The model has been modified to account for a new feature in the assembly kinetics revealed by time-course light scattering experiments at higher temporal resolution than previously attempted. To be able to simulate the sharp takeoff from the initial lag phase to the growth phase in the kinetic curves, a monomer activation step was proposed.

[1]  K. Kirkegaard,et al.  Potential subversion of autophagosomal pathway by picornaviruses , 2008, Autophagy.

[2]  A. Zlotnick,et al.  To build a virus capsid. An equilibrium model of the self assembly of polyhedral protein complexes. , 1994, Journal of molecular biology.

[3]  A. Zlotnick,et al.  Mechanism of capsid assembly for an icosahedral plant virus. , 2000, Virology.

[4]  M. Lewis,et al.  Calculation of the free energy of association for protein complexes , 1992, Protein science : a publication of the Protein Society.

[5]  Z. Kam,et al.  Absorption and Scattering of Light by Small Particles , 1998 .

[6]  W. Kegel,et al.  Competing hydrophobic and screened-coulomb interactions in hepatitis B virus capsid assembly. , 2004, Biophysical journal.

[7]  Paul Ahlquist,et al.  Three-Dimensional Analysis of a Viral RNA Replication Complex Reveals a Virus-Induced Mini-Organelle , 2007, PLoS biology.

[8]  Adam Zlotnick,et al.  Theoretical aspects of virus capsid assembly , 2005, Journal of molecular recognition : JMR.

[9]  E. Hiebert,et al.  Factors affecting the assembly of some spherical viruses. , 1969, Virology.

[10]  Kristin N. Parent,et al.  Phage P22 procapsids equilibrate with free coat protein subunits. , 2007, Journal of molecular biology.

[11]  David Reguera,et al.  Classical nucleation theory of virus capsids. , 2006, Biophysical journal.

[12]  S. Stahl,et al.  A theoretical model successfully identifies features of hepatitis B virus capsid assembly. , 1999, Biochemistry.

[13]  Fumio Oosawa,et al.  Thermodynamics of the polymerization of protein , 1975 .

[14]  S. Harrison,et al.  Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16. , 2000, Molecular cell.

[15]  J M Kenney,et al.  Evolutionary conservation in the hepatitis B virus core structure: comparison of human and duck cores. , 1995, Structure.

[16]  Adam Zlotnick,et al.  Observed Hysteresis of Virus Capsid Disassembly Is Implicit in Kinetic Models of Assembly* , 2003, The Journal of Biological Chemistry.

[17]  A. Zlotnick,et al.  Weak protein-protein interactions are sufficient to drive assembly of hepatitis B virus capsids. , 2002, Biochemistry.

[18]  J. King,et al.  Assembly in vitro of bacteriophage P22 procapsids from purified coat and scaffolding subunits. , 1982, Journal of molecular biology.

[19]  Adam Zlotnick,et al.  Distinguishing reversible from irreversible virus capsid assembly. , 2007, Journal of molecular biology.

[20]  G. Némethy,et al.  Hydrophobic interactions. , 1967, Angewandte Chemie.

[21]  B. Berger,et al.  Local rules simulation of the kinetics of virus capsid self-assembly. , 1998, Biophysical journal.

[22]  RNA viruses: stabilization of brome mosaic virus. , 1976, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[23]  R. Garcea,et al.  In vitro papillomavirus capsid assembly analyzed by light scattering. , 2004, Virology.

[24]  Adam Zlotnick,et al.  Are weak protein-protein interactions the general rule in capsid assembly? , 2003, Virology.

[25]  C. Bracker,et al.  The self-assembly of a nucleic-acid free pseudo-top component for a small spherical virus. , 1968, Virology.

[26]  S. Larson,et al.  The crystallographic structure of brome mosaic virus. , 2002, Journal of molecular biology.

[27]  J. King,et al.  Scaffolding protein regulates the polymerization of P22 coat subunits into icosahedral shells in vitro. , 1988, Journal of molecular biology.

[28]  P. Pfeiffer,et al.  Aggregation states of brome mosaic virus protein. , 1974, Virology.

[29]  M. Zulauf,et al.  Self-assembly of brome mosaic virus protein into capsids. Initial and final states of aggregation. , 1983, Journal of Molecular Biology.

[30]  C. Brooks,et al.  Deciphering the kinetic mechanism of spontaneous self-assembly of icosahedral capsids. , 2007, Nano letters (Print).

[31]  D. Rapaport,et al.  Self-assembly of polyhedral shells: a molecular dynamics study. , 2004, Physical review. E, Statistical, nonlinear, and soft matter physics.

[32]  A. Zlotnick,et al.  Effects of the cowpea chlorotic mottle bromovirus beta-hexamer structure on virion assembly. , 2003, Virology.

[33]  B Berger,et al.  Local rule-based theory of virus shell assembly. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[34]  Stabilization of brome mosaic virus , 1976 .

[35]  J. King,et al.  Nucleation and growth phases in the polymerization of coat and scaffolding subunits into icosahedral procapsid shells. , 1993, Biophysical journal.

[36]  P. Vachette,et al.  Self-assembly of brome mosaic virus capsids. Kinetic study using neutron and X-ray solution scattering. , 1983, Journal of molecular biology.

[37]  V S Reddy,et al.  Energetics of quasiequivalence: computational analysis of protein-protein interactions in icosahedral viruses. , 1998, Biophysical journal.