In situ biodiesel production from wet Chlorella vulgaris under subcritical condition

Abstract The conventional base catalyzed biodiesel production process uses refined vegetable oil as feedstock oil and is not environmentally friendly. The supercritical methanol technology does not require the use of catalyst but it is energy intensive due to the high temperature and pressure required in the process. In this work, a process was developed for producing biodiesel directly from wet Chlorella vulgaris biomass (80% moisture content) using subcritical water as catalyst. Under the following conditions: The ratio of wet biomass to methanol is 1/4 (g/mL), the reaction temperature is 175 °C and after 4 h, the reaction product contained 89.71% fatty acid methyl esters (FAMEs). The yield is 0.29 g FAME per g dry biomass. This is considerably higher than the yield of 0.20 g FAME per g dry biomass obtained when the neutral lipid of C. vulgaris biomass was extracted and converted into FAME.

[1]  Y. Chisti.  Biodiesel from microalgae. , 2007, Biotechnology advances.

[2]  Milford A. Hanna,et al.  The effect of mixing on transesterification of beef tallow , 1999 .

[3]  L. Lascaray.  Mechanism of Fat Splitting , 1949 .

[4]  Phillip E. Savage,et al.  Biodiesel Production from Wet Algal Biomass through in Situ Lipid Hydrolysis and Supercritical Transesterification , 2010 .

[5]  A. Demirbas,et al.  Biodiesel from waste cooking oil via base-catalytic and supercritical methanol transesterification , 2009 .

[6]  Koichi Fujie,et al.  Low-molecular-weight carboxylic acids produced from hydrothermal treatment of organic wastes. , 2002, Journal of hazardous materials.

[7]  R. M. Willis,et al.  Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures. , 2011, Bioresource technology.

[8]  Maarten Boersma,et al.  Extraction of pigments and fatty acids from the green alga Scenedesmus obliquus (Chlorophyceae) , 2000, Aquatic Ecology.

[9]  Dadan Kusdiana,et al.  Effects of water on biodiesel fuel production by supercritical methanol treatment. , 2004, Bioresource technology.

[10]  Y. Ju,et al.  Extraction and analysis of neutral lipids from activated sludge with and without sub-critical water pre-treatment. , 2010, Bioresource technology.

[11]  Adam Harvey,et al.  Alkaline in situ transesterification of Chlorella vulgaris , 2012 .

[12]  P. Webley,et al.  Extraction of oil from microalgae for biodiesel production: A review. , 2012, Biotechnology advances.

[13]  Z. Xiu,et al.  Biodiesel Production from Subcritical Methanol Transesterification of Soybean Oil with Sodium Silicate , 2010 .

[14]  Jian-Zhong Yin,et al.  Biodiesel from soybean oil in supercritical methanol with co-solvent. , 2008 .

[15]  Jo-Shu Chang,et al.  Enzymatic transesterification of microalgal oil from Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized Burkholderia lipase. , 2012, Bioresource technology.

[16]  J. King,et al.  Hydrolysis of Vegetable Oils in Sub- and Supercritical Water , 1997 .

[17]  Nick Nagle,et al.  Production of methyl ester fuel from microalgae , 1990 .

[18]  Lawrence L. Tavlarides,et al.  Investigations on supercritical transesterification of chicken fat for biodiesel production from low-cost lipid feedstocks , 2010 .

[19]  Dadan Kusdiana,et al.  Kinetics of transesterification in rapeseed oil to biodiesel fuel as treated in supercritical methanol , 2001 .

[20]  Yi-Hsu Ju,et al.  Synthesis of biodiesel in subcritical water and methanol , 2013 .

[21]  X. Miao,et al.  Biodiesel production from heterotrophic microalgal oil. , 2006, Bioresource technology.

[22]  H. Oh,et al.  Comparison of several methods for effective lipid extraction from microalgae. , 2010, Bioresource technology.

[23]  Tao Wang,et al.  Continuous production of biodiesel fuel from vegetable oil using supercritical methanol process , 2007 .

[24]  Réjean Tremblay,et al.  Effect of ultrasonication and grinding on the determination of lipid class content of microalgae harvested on filters , 2003, Lipids.

[25]  Yongli Mi,et al.  Simplifying the Process of Microalgal Biodiesel Production Through In Situ Transesterification Technology , 2011 .

[26]  Gerd Brunner,et al.  Production of amino acids from bovine serum albumin by continuous sub-critical water hydrolysis , 2005 .

[27]  Y. Ju,et al.  Maximizing biodiesel production from Yarrowia lipolytica Po1g biomass using subcritical water pretreatment. , 2012, Bioresource technology.

[28]  Y. Ju,et al.  Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate. , 2011, Bioresource technology.

[29]  Q. Hu,et al.  Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. , 2008, The Plant journal : for cell and molecular biology.

[30]  Jo‐Shu Chang,et al.  Effect of light supply and carbon source on cell growth and cellular composition of a newly isolated microalga Chlorella vulgaris ESP‐31 , 2010 .

[31]  S. Adachi,et al.  Kinetics on the hydrolysis of fatty acid esters in subcritical water , 2004 .

[32]  M. Huntley,et al.  CO2 Mitigation and Renewable Oil from Photosynthetic Microbes: A New Appraisal , 2007 .

[33]  A. Demirbas,et al.  Biodiesel production via non-catalytic SCF method and biodiesel fuel characteristics. , 2006 .

[34]  Sulaiman Al‐Zuhair,et al.  Production of biodiesel: possibilities and challenges , 2007 .

[35]  C. Carrington,et al.  Variables affecting the in situ transesterification of microalgae lipids , 2010 .

[36]  Hong-shik Lee,et al.  Transesterification of RBD palm oil using supercritical methanol , 2008 .

[37]  Parisa A. Bahri,et al.  Supercritical methanol for fatty acid methyl ester production: A review , 2011 .

[38]  R. Ruan,et al.  Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials , 2011 .

[39]  Georgi Petkov,et al.  Which are fatty acids of the green alga Chlorella , 2007 .

[40]  Ali O. Al-Shyoukh,et al.  Experimental evaluation of the transesterification of waste palm oil into biodiesel. , 2002, Bioresource technology.