Endogenous gibberellins in Lolium perenne and influence of defoliation on their contents in elongating leaf bases and in leaf sheaths

Gibberellin (GA) levels in leaf sheaths and in elongating leaf bases of perennial ryegrass (Lolium perenne L. cv. Bravo) were monitored in undefoliated and defoliated plants. Nine C-13-hydroxylated GAs (GA 8 , GA 97 , GA 29 , GA 1 , GA 20 , GA 44 , GA 19 , GA 17 , GA 53 ) and one C-13-non-hydroxylated GA (GA 9 ) were identified by combined gas chromatography-mass spectrometry in leaf extracts. The total level of GA 8 , GA 29 , GA 1 , GA 20 , GA 44 , GA 19 and GA 53 , determined by selected ion monitoring, was 7 times higher in elongating leaf bases than in mature leaf sheaths. In both leaf tissues, defoliation induced an increase in GA 53 level, while GA 20 and GA 1 levels decreased, suggesting that the GA 53 → GA 44 , as well as GA 19 → GA 20 , conversions were slowed down. The roles of GA 1 in the control of leaf elongation and fructan mobilization following defoliation are discussed.

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