The metabolism of nicotine and cotinine by a mouse liver preparation.

The metabolism of nicotine and cotinine by a 10,000 ×g supernatant fraction of mouse liver homogenate has been studied by using 14C-labelled compounds. The metabolism of nicotine was found to be TPNH and O2 dependent and chromatographical evidences for the formation of cotinine, γ-(3-pyridy 1)-γ-oxo-N-methylbutyramide and hydroxycotinine are presented. Three unidentified metabolites and 14CO2 were also observed. The observed metabolites were also excreted in urine after intraperitoneal administration of nicotine. Chromatographical evidences revealed that cotinine is metabolized to hydroxycotinine, γ-(3-pyridyl)-γ-oxo-N-methylbutyr-amide and/or demethylcotinine and one unidentified metabolite. No formation of 14O2 was observed.

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