Enzymatic Synthesis of Carbohydrate Derivatives Using β-Glycosidase of Sulfolobus Solfataricus

Enzymatic synthesis of different β-D-glycosides was obtained using as biocatalyst immobilized cells, crude homogenate, and homogeneous native and recombinat β-glycosidase activity of the thermophilic archaeon Sulfolobus solfataricus. In particular our investigation was concerned with the selectivity in the glycosylation of hydroxybenzyl alcohols, salicin, 1,2-propanediol, and more complex polyols as well as the use of immobilized cells for the synthesis of hexyl-β-D-glucoside. The aromatic glucosides obtained by enzyme-catalyzed transglucosylation were used for kinetic studies of purified Sulfolobus solfataricus enzyme in the hydrolysis reaction.

[1]  A. Gambacorta,et al.  Potential Application of Sulfolobus solfataricus as Catalyst in Organic Synthesis. , 1993 .

[2]  M. Rossi,et al.  Exo‐glucosidase activity and substrate specificity of the beta‐glycosidase isolated from the extreme thermophile Sulfolobus solfataricus , 1993, Biotechnology and applied biochemistry.

[3]  A. Fernández-Mayoralas,et al.  Controlling yield and regioselectivity in the enzymatic synthesis of β-D-galactopyranosyl-β-D-xylopyranosides , 1992 .

[4]  Daniel Thomas,et al.  Enzyme-catalysed synthesis of alkyl β-D-Glucosides in organic media. , 1992 .

[5]  M. Rossi,et al.  Expression of the thermostable beta-galactosidase gene from the archaebacterium Sulfolobus solfataricus in Saccharomyces cerevisiae and characterization of a new inducible promoter for heterologous expression , 1992, Journal of bacteriology.

[6]  D. Grogan.  Evidence that β-Galactosidase of Sulfolobus solfataricus Is Only One of Several Activities of a Thermostable β-d-Glycosidase , 1991 .

[7]  A. Gambacorta,et al.  Stereochemical studies of enzymatic transglycosylation using Sulfolobus solfataricus , 1991 .

[8]  M. Rossi,et al.  Enzymes from Extreme Thermophilic Bacteria as Special Catalysts: Studies on a β-Galactosidase from Sulfolobus solfataricus , 1991 .

[9]  T. Furuya,et al.  Triglucosylation on the biotransformation of (+)-menthol by cultured cells of Eucalyptus perriniana. , 1991, Phytochemistry.

[10]  M. Cubellis.  Isolation and sequencing of a new -galactosidase-encoding archaebacterial gene , 1990 .

[11]  R. Rella,et al.  Thermostable β-galactosidase from the archaebacterium Sulfolobus solfataricus Purification and properties , 1990 .

[12]  P. Critchley,et al.  Enzymatic synthesis of glycosides using the β-galactosidase of Escherichia coli: regio- and stereo-chemical studies , 1990 .

[13]  M. Kaneda.  Lilioside a from Lilium longiflorum: Synthesis and absolute configuration , 1990 .

[14]  S. Godtfredsen,et al.  New enzyme catalyzed synthesis of monoacyl galactoglycerides , 1988 .

[15]  R. E. Huber,et al.  Binding and reactivity at the "glucose" site of galactosyl-beta-galactosidase (Escherichia coli). , 1984, Archives of biochemistry and biophysics.

[16]  T. Satoh,et al.  Synthesis of β-Alkyl Glucosides by Enzymic Transglucosylaiton , 1984 .

[17]  Y. Tomita,et al.  Determination of the absolute configuration of a secondary hydroxy group in a chiral secondary alcohol using glycosidation shifts in carbon-13 nuclear magnetic resonance spectroscopy , 1978 .

[18]  M. de Rosa,et al.  Extremely thermophilic acidophilic bacteria convergent with Sulfolobus acidocaldarius. , 1975, Journal of general microbiology.