论文信息 - DNA sensor based on functionalized polypyrrole

DNA sensor based on functionalized polypyrrole

Abstract A novel DNA sensor based on a functionalized polypyrrole was prepared. The sensor film was obtained by electrocopolymerizing poly(pyrrole- co -4-(3-pyrrolyl) butanoic acid) onto which an NH 2 -ODN probe was grafted. The obtained sensor is a simple, label-free DNA sensor with direct electrical read-out. Hybridisation with complementary ODN was investigated by observing changes in die cyclic voltammogram and the AC impedance spectrum. Significant modifications of both the voltammogram and AC impedance spectrum were observed when the sensor was incubated in the presence of complementary ODN. The AC impedance spectra show an increased charge transfer resistance of the film after hybridisation.

[1] T. G. Drummond,et al. Electrochemical DNA sensors , 2003, Nature Biotechnology.

[2] S. Cosnier. Biosensors based on immobilization of biomolecules by electrogenerated polymer films , 2000, Applied biochemistry and biotechnology.

[3] Thierry Delair,et al. Toward intelligent polymers: DNA sensors based on oligonucleotide-functionalized polypyrroles , 1999 .

[4] Thierry Livache,et al. Electronically conductive polymer grafted with oligonucleotides as electrosensors of DNA: Preliminary study of real time monitoring by in situ techniques , 2001 .

[5] A. Heeger. Charge Storage in Conducting Polymers: Solitons, Polarons, and Bipolarons , 1985 .

[6] Y. Shim,et al. Direct DNA hybridization detection based on the oligonucleotide-functionalized conductive polymer. , 2001, Analytical chemistry.

[7] B. Weimer,et al. Optimizing the immobilization of single-stranded DNA onto glass beads. , 2001, Journal of biochemical and biophysical methods.