In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats

Control of infection in wounds is critically important to avoid transition to sepsis; however, recent rise of drug-resistant bacteria makes it difficult. Thus, antimicrobial photodynamic therapy (APDT) has recently received considerable attention. In this study, we examined methylene blue (MB)-mediated photodynamic inactivation of Psuedomonas aeruginosa in rat burned skin. Two days after infection, the wound surface was contacted with a MB solution at different concentrations, and thereafter the wound was irradiated with cw 665-nm light at a constant power density of 250 mW/cm2 for different time durations. We obtained a two orders of magnitude decrease in the number of bacteria by PDT with a 2-h contact of 0.5-mM MB solution and a illumination of 480 J/cm2, demonstrating the efficacy of PDT against infection with Ps. aeruginosa in burns.

[1]  C. Street,et al.  In Vitro Photodynamic Eradication of Pseudomonas aeruginosa in Planktonic and Biofilm Culture , 2009, Photochemistry and photobiology.

[2]  A. Mason,et al.  Direct current reduces plasma protein extravasation after partial-thickness burn injury in rats. , 1996, The Journal of trauma.

[3]  S. Zahler,et al.  An angiogenic role for the human peptide antibiotic LL-37/hCAP-18. , 2003, The Journal of clinical investigation.

[4]  J. Lawrence.  Burn bacteriology during the last 50 years. , 1992, Burns : journal of the International Society for Burn Injuries.

[5]  Michael R Hamblin,et al.  Phenothiazinium Antimicrobial Photosensitizers Are Substrates of Bacterial Multidrug Resistance Pumps , 2006, Antimicrobial Agents and Chemotherapy.

[6]  Tayyaba Hasan,et al.  Photodynamic therapy for Staphylococcus aureus infected burn wounds in mice , 2005, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[7]  A. Mason,et al.  Multiple graft harvestings from deep partial-thickness scald wounds healed under the influence of weak direct current. , 1990, The Journal of trauma.

[8]  G. Su,et al.  Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns , 2002, Antimicrobial Agents and Chemotherapy.

[9]  R. Ghiladi,et al.  Highly efficient in vitro photodynamic inactivation of Mycobacterium smegmatis. , 2009, The Journal of antimicrobial chemotherapy.

[10]  J. C. Junqueira,et al.  Photodynamic therapy for the treatment of buccal candidiasis in rats , 2009, Lasers in Medical Science.

[11]  E. Munin,et al.  Comparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicans , 2010, Lasers in Medical Science.

[12]  Cale Street,et al.  In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent , 2009, BMC Microbiology.

[13]  A. Mason,et al.  A standard animal burn. , 1968, The Journal of trauma.

[14]  Michael R Hamblin,et al.  Photodynamic Therapy for Acinetobacter baumannii Burn Infections in Mice , 2009, Antimicrobial Agents and Chemotherapy.

[15]  F. Jacobsen,et al.  Transient cutaneous adenoviral gene therapy with human host defense peptide hCAP-18/LL-37 is effective for the treatment of burn wound infections , 2005, Gene Therapy.

[16]  Z. Malik,et al.  The use of porphyrins for eradication of Staphylococcus aureus in burn wound infections. , 1997, FEMS immunology and medical microbiology.