Characterization of a laser speckle imaging instrument for monitoring skin blood flow dynamics

Noninvasive monitoring of a therapeutic intervention is desired to provide the clinician or scientist with insight into the efficacy of the intervention. Since blood flow is tightly coupled into the health status of biological tissue, several instruments have been developed to monitor blood flow and perfusion dynamics. One such instrument is laser speckle imaging (LSI). The goal of this study was to characterize the relationship between speckle flow index (SFI) and actual flow rate. To achieve this goal, we employed an in vitro flow model. With whole blood as the flow fluid, we found that the actual flow rate and SFI had a linear relationship (R = 0.99) over a physiologically relevant 0 to 5 mm/s range. From our data it appears that a predictive model from the literature underestimates considerably the actual dynamic range of LSI for flow imaging.

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