Robust sensing films for pathogen detection and medical diagnostics

Our team has developed polyethylene glycol (PEG)-modified, self-assembled monolayers (SAMs) for biological detection on either planar or spherical substrates, which resist non-specific binding while facilitating specific ligand attachment. The preparation and characterization of these thin films, their validation against B. anthracis protective antigen (PA) in a sandwich assay format, and the application of these thin films for quantitative analysis of several medically interesting targets (breast cancer, tuberculosis, and influenza) will be shown.

[1]  Jin Ho Kim,et al.  Absolute Surface Density of the Amine Group of the Aminosilylated Thin Layers: Ultraviolet−Visible Spectroscopy, Second Harmonic Generation, and Synchrotron-Radiation Photoelectron Spectroscopy Study , 1997 .

[2]  Tejal A Desai,et al.  Evaluation of the stability of nonfouling ultrathin poly(ethylene glycol) films for silicon-based microdevices. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[3]  M. R. Sweet,et al.  Reagentless optical biosensor , 2004, SPIE Optics East.

[4]  G. Whitesides,et al.  Self-assembled organic monolayers: model systems for studying adsorption of proteins at surfaces , 1991, Science.

[5]  Alexander Papra,et al.  Characterization of ultrathin poly(ethylene glycol) monolayers on silicon substrates , 2001 .

[6]  G. Whitesides,et al.  Formation of self-assembled monolayers by chemisorption of derivatives of oligo(ethylene glycol) of structure HS(CH2)11(OCH2CH2)mOH on gold , 1991 .

[7]  Aaron S. Anderson,et al.  Functional PEG-modified thin films for biological detection. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[8]  R. Raiteri,et al.  Adsorption of trimethoxysilane and of 3-mercaptopropyltrimethoxysilane on silica and on silicon wafers from vapor phase: an IR study. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[9]  Harshini Mukundan,et al.  Detection of intact influenza viruses using biotinylated biantennary S-sialosides. , 2008, Journal of the American Chemical Society.

[10]  C. Mandenius,et al.  Gas phase silylation, a rapid method for preparation of high-performance liquid chromatography supports , 1988 .

[11]  Tejal A Desai,et al.  Nanostructured antifouling poly(ethylene glycol) films for silicon-based microsystems. , 2005, Journal of nanoscience and nanotechnology.

[12]  Harshini Mukundan,et al.  Optimizing a waveguide-based sandwich immunoassay for tumor biomarkers: evaluating fluorescent labels and functional surfaces. , 2009, Bioconjugate chemistry.

[13]  P. Voort,et al.  Characterization and Chemical Modification of the Silica Surface , 1995 .

[14]  V. Basiuk,et al.  Gas-phase synthesis, properties and some applications of acylamide stationary phases for high-performance liquid chromatography , 1990 .

[15]  P. Messersmith,et al.  Protein resistance of titanium oxide surfaces modified by biologically inspired mPEG-DOPA. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[16]  Hongwei Ma,et al.  Surface‐Initiated Atom Transfer Radical Polymerization of Oligo(ethylene glycol) Methyl Methacrylate from a Mixed Self‐Assembled Monolayer on Gold , 2006 .

[17]  G. Whitesides,et al.  Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide): a model system using self-assembled monolayers , 1993 .

[18]  B. Clare,et al.  Covalently modified silicon and diamond surfaces: resistance to nonspecific protein adsorption and optimization for biosensing. , 2004, Journal of the American Chemical Society.

[19]  K. M. Grace,et al.  Integrated optical biosensor for detection of multivalent proteins. , 1999, Optics letters.

[20]  White,et al.  Reaction of (3-Aminopropyl)dimethylethoxysilane with Amine Catalysts on Silica Surfaces. , 2000, Journal of colloid and interface science.

[21]  Yuchun Wang,et al.  Surface modification of micromachined silicon filters , 2000 .

[22]  D. Castner,et al.  Biomedical surface science: Foundations to frontiers , 2002 .

[23]  Aaron S. Anderson,et al.  Functionalized Waveguides for Biological Assays , 2006 .

[24]  T. Bein,et al.  Thin Films of (3-Aminopropyl)triethoxysilane on Aluminum Oxide and Gold Substrates , 1995 .

[25]  M. Textor,et al.  Covalent Attachment of Novel Poly(ethylene glycol)-Poly(DL-lactic acid) Copolymeric Micelles to TiO2 Surfaces , 2002 .

[26]  Tuomo Suntola,et al.  Atomic Layer Epitaxy , 1984 .

[27]  T. Desai,et al.  Controlling Nonspecific Protein Interactions in Silicon Biomicrosystems with Nanostructured Poly(ethylene glycol) Films , 2002 .

[28]  T. Pakkanen,et al.  Atomic layer deposition of a high-density aminopropylsiloxane network on silica through sequential reactions of γ-aminopropyltrialkoxysilanes and water , 2003 .

[29]  D. Kremer,et al.  A Numerical Investigation of the Effects of Gas-Phase Particle Formation on Silicon Film Deposition from Silane , 2003 .

[30]  P. E. Laibinis,et al.  Protein-resistant coatings for glass and metal oxide surfaces derived from oligo(ethylene glycol)-terminated alkytrichlorosilanes. , 1998, Biomaterials.

[31]  T. Bein,et al.  Surface reactions on thin layers of silane coupling agents , 1993 .

[32]  Marcus Textor,et al.  Nitrilotriacetic Acid Functionalized Graft Copolymers: A Polymeric Interface for Selective and Reversible Binding of Histidine‐Tagged Proteins , 2006 .

[33]  R. Glockshuber,et al.  Immobilization of the enzyme beta-lactamase on biotin-derivatized poly(L-lysine)-g-poly(ethylene glycol)-coated sensor chips: a study on oriented attachment and surface activity by enzyme kinetics and in situ optical sensing. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[34]  L. Niinistö,et al.  Gas-Phase Deposition of Aminopropylalkoxysilanes on Porous Silica , 2003 .

[35]  Haiwon Lee,et al.  Ultraviolet-ozone jet cleaning process of organic surface contamination layers , 1999 .

[36]  M. Textor,et al.  Effects of ionic strength and surface charge on protein adsorption at PEGylated surfaces. , 2005, The journal of physical chemistry. B.

[37]  Gil U. Lee,et al.  Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol). , 2007, Langmuir : the ACS journal of surfaces and colloids.

[38]  E. Lakomaa.  Atomic layer epitaxy (ALE) on porous substrates , 1994 .

[39]  J. Youngblood,et al.  Optimization of silica silanization by 3-aminopropyltriethoxysilane. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[40]  R. Meagher,et al.  A very thin coating for capillary zone electrophoresis of proteins based on a tri(ethylene glycol)‐terminated alkyltrichlorosilane , 2004, Electrophoresis.

[41]  M. Ferrari,et al.  Proteins and cells on PEG immobilized silicon surfaces. , 1998, Biomaterials.

[42]  Basil I. Swanson,et al.  Pathogen detection using single mode planar optical waveguides , 2005 .

[43]  Jihoon Shin,et al.  Formation of Uniform Aminosilane Thin Layers: An Imine Formation To Measure Relative Surface Density of the Amine Group , 1996 .

[44]  Jinho Hyun,et al.  “Non‐Fouling” Oligo(ethylene glycol)‐ Functionalized Polymer Brushes Synthesized by Surface‐Initiated Atom Transfer Radical Polymerization , 2004 .