Frequency of precursor cells against the enzyme β‐galactosidase An estimate of the BALB/c strain antibody repertoire

A method has been developed for detecting anti‐β‐galactosidase antibodies after isoelectric focusing in thin layers of polyacrylamide gel. By “staining” with wild‐type enzyme, all antibodies against β‐galactosidase are detected, while a subset of antibodies able to activate a mutant enzyme is detected by staining with that enzyme.

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