Soybean leaf nitrogen, chlorophyll content, and chlorophyll a/b ratio

The objective of this study was to assess genotypic variation in soybean chlorophyll (Chl) content and composition, and to test if these data could be used as a rapid screening method to predict genotypic variation in leaf tissue N content. Chl contents and composition were examined among 833 soybean (Glycine max L. Merr.) accessions and related to SPAD meter readings and leaf N content. In the initial year of the study (2002), the relationship between leaf Chl and leaf N contents (r2 = 0.043) was not sufficiently close for Chl to be useful as a predictive tool for leaf N content. Therefore, leaf N content was not determined in 2004 but samples were again collected for determination of Chl content and composition. In 2002, the soybean accessions separated into two distinct groups according to leaf Chl a/b ratios, with the majority of a mean ratio of 3.79. However, approximately 7 % (60) of the genotypes could be readily assigned to a group with a mean Chl a/b ratio of 2.67. Chl a/b analyses in 2004 confirmed the results obtained in 2002 and of 202 genotypes, all but 6 fell into the same group as in 2002.

[1]  J. Wintermans,et al.  Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanol , 1965 .

[2]  W. Fehr,et al.  Stage of Development Descriptions for Soybeans, Glycine Max (L.) Merrill , 1971 .

[3]  S. W. Thorne,et al.  Effect of light intensity during growth of Atriplex patula on the capacity of photosynthetic reactions, chloroplast components and structure , 1972 .

[4]  N. Boardman.  Comparative photosynthesis of sun and shade plants. , 1977 .

[5]  W. K. Robertson,et al.  Effect of Foliar Fertilization on Photosynthesis, Leaf Nutrition, and Yield of Soybeans1 , 1978 .

[6]  D. G. Lugg,et al.  Seasonal changes in photosynthesis of field-grown soybean leaflets. 2. Relation to nitrogen content , 1981 .

[7]  W. Findlay,et al.  RELATIONSHIPS AMONG PHOTOSYNTHETIC RATE, BEAN YIELD AND OTHER CHARACTERS IN FIELD-GROWN CULTIVARS OF SOYBEAN , 1981 .

[8]  D. Egli,et al.  Leaf N and Photosynthesis during Reproductive Growth in Soybeans 1 , 1983 .

[9]  Z. Šesták.  Chlorophylls and Carotenoids during Leaf Ontogeny , 1985 .

[10]  I. Terashima,et al.  Palisade Tissue Chloroplasts and Spongy Tissue Chloroplasts in Spinach: Biochemical and Ultrastructural Differences , 1985 .

[11]  I. Terashima,et al.  Intra-Leaf and Intracellular Gradients in Chloroplast Ultrastructure of Dorsiventral Leaves Illuminated from the Adaxial or Abaxial Side during their Development , 1986 .

[12]  J. Anderson,et al.  Photoregulation of the Composition, Function, and Structure of Thylakoid Membranes , 1986 .

[13]  U. L. Yadawa,et al.  A Rapid and Nondestructive Method to Determine Chlorophyll in Intact Leaves , 1986, HortScience.

[14]  R. Marquard,et al.  Relationship Between Extractable Chlorophyll and an in Situ Method to Estimate Leaf Greenness , 1987, HortScience.

[15]  R. Buzzell,et al.  SOYBEAN LEAF NITROGEN IN RELATION TO PHOTOSYNTHETIC RATE AND YIELD , 1988 .

[16]  William K. Smith,et al.  Chlorophyll and light gradients in sun and shade leaves of Spinacia oleracea , 1991 .

[17]  D. R. Causton,et al.  Use of the chlorophyll a/b ratio as a bioassay for the light environment of a plant , 1992 .

[18]  C. W. Wood,et al.  Determination of wheat nitrogen status with a hand‐held chlorophyll meter: Influence of management practices 1 , 1993 .

[19]  L. Natr,et al.  Chlorophyll a fluorescence signatures of nitrogen deficient barley leaves , 1993 .

[20]  Kenneth G. Cassman,et al.  Adjustment for Specific Leaf Weight Improves Chlorophyll Meter's Estimate of Rice Leaf Nitrogen Concentration , 1993 .

[21]  B. Green,et al.  THE CHLOROPHYLL-CAROTENOID PROTEINS OF OXYGENIC PHOTOSYNTHESIS. , 1996, Annual review of plant physiology and plant molecular biology.

[22]  Larry G. Heatherly,et al.  Soybean Production in the Midsouth , 1998 .

[23]  Wu Feibo,et al.  Chlorophyll meter to predict nitrogen sidedress requirements for short-season cotton (Gossypium hirsutum L.) , 1998 .

[24]  Donald G. Bullock,et al.  Evaluation of the Minolta SPAD-502 chlorophyll meter for nitrogen management in corn , 1998 .

[25]  Ronald W. McNew,et al.  Comparison of plant measurements for estimating nitrogen accumulation and grain yield by flooded rice , 1999 .

[26]  R. Rao,et al.  Rapid Assessment of Specific Leaf Area and Leaf Nitrogen in Peanut (Arachis hypogaea L.) using a Chlorophyll Meter , 2001 .

[27]  R. Rao,et al.  Rapid Assessment of Speci ® c Leaf Area and Leaf Nitrogen in Peanut ( Arachis hypogaea L . ) using a Chlorophyll , 2001 .

[28]  K. Kitajima,et al.  Increases of chlorophyll a/b ratios during acclimation of tropical woody seedlings to nitrogen limitation and high light. , 2003, Plant, cell & environment.

[29]  Scott X. Chang,et al.  Nondestructive and rapid estimation of hardwood foliar nitrogen status using the SPAD-502 chlorophyll meter , 2003 .

[30]  Randall L. Nelson,et al.  Association of specific leaf weight, an estimate of chlorophyll, and chlorophyll concentration with apparent photosynthesis in soybean , 1996, Photosynthesis Research.

[31]  Jan M. Anderson,et al.  Adaptation of the thylakoid membranes of pea chloroplasts to light intensities. I. Study on the distribution of chlorophyll-protein complexes , 1984, Photosynthesis Research.

[32]  D. Meier,et al.  Photosynthetic activity, chloroplast ultrastructure, and leaf characteristics of high-light and low-light plants and of sun and shade leaves , 1981, Photosynthesis Research.

[33]  J. R. Evans.  Photosynthesis and nitrogen relationships in leaves of C3 plants , 2004, Oecologia.

[34]  J. Hesketh,et al.  Correlations among leaf CO2-exchange rates, areas and enzyme activities among soybean cultivars , 1981, Photosynthesis Research.

[35]  G. Mauromicale,et al.  Chlorophyll fluorescence and chlorophyll content in field-grown potato as affected by nitrogen supply, genotype, and plant age , 2006, Photosynthetica.